Glycerol conversion to 1,3-propanediol by Clostridium pasteurianum: cloning and expression of the gene encoding 1,3-propanediol dehydrogenase

Luers, Frauke; Seyfried, Markus; Daniel, Rolf; Gottschalk, Gerhard

doi:10.1111/j.1574-6968.1997.tb12665.x

Cited by 67 publications

(19 citation statements)

References 32 publications

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“…Biological synthesis of 1,3-PD has been demonstrated in several anaerobic and microaerophilic bacteria including microorganisms such as Klebsiella, Citrobacter, and Clostridium (6,11,23) and also microorganisms belonging to the genus Lactobacillus (Lactobacillus brevis, L. reuterii, L. sakei, L. plantarum, and L. buchneri) (40)(41)(42) as a product of glycerol fermentation not found in anaerobic conversion of other substrates. In these organisms, glycerol is metabolized by two different parallel pathways.…”

mentioning

confidence: 99%

1,3-Propanediol Dehydrogenase fromKlebsiella pneumoniae: Decameric Quaternary Structure and Possible Subunit Cooperativity

et al. 2009

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Klebsiella pneumoniae is a nosocomial pathogen frequently isolated from opportunistic infections, especially in clinical environments. In spite of its potential pathogenicity, this microorganism has several metabolic potentials that could be used in biotechnology applications. K. pneumoniae is able to metabolize glycerol as a sole source of carbon and energy. 1,3-Propanediol dehydrogenase is the core of the metabolic pathway for the use of glycerol. We have determined the crystallographic structure of 1,3-propanediol dehydrogenase, a type III Fe-NAD-dependent alcohol dehydrogenase, at 2.7-Å resolution. The structure of the enzyme monomer is closely related to that of other alcohol dehydrogenases. The overall arrangement of the enzyme showed a decameric structure, formed by a pentamer of dimers, which is the catalytic form of the enzyme. Dimers are associated by strong ionic interactions that are responsible for the highly stable in vivo packing of the enzyme. Kinetic properties of the enzyme as determined in the article would suggest that this decameric arrangement is related to the cooperativity between monomers.

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confidence: 99%

1,3-Propanediol Dehydrogenase fromKlebsiella pneumoniae: Decameric Quaternary Structure and Possible Subunit Cooperativity

et al. 2009

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“…It was assumed that the first step in an aerobic pathway for degradation of 1,3-propanediol involved an oxidoreductase because (i) this group of enzymes catalyzing oxidation/reduction reactions are often involved in the early stages of degradation of many organic compounds and (ii) a dehydrogenase specific for 1,3-propanediol (albeit having activity in the reverse direction) has been identified in the pathway for production of 1,3-propanediol from glucose and glycerol (Daniel et al, 1995;Luers et al, 1997;Veiga-da-Cunha and Foster, 1992). By utilizing a feedback loop linked to BOD, it was anticipated that an increase in the feed flow (i.e., dilution rate) would result in selection of microbes that had high 1,3-propanediol dehydrogenase activity [i.e., microorganisms with a higher enzyme activity would be expected to proliferate at higher dilution rates (high feed flows)].…”

Section: Use Of the Oxygen Uptake Rate To Facilitate Enzyme Selectionmentioning

confidence: 99%

The use of oxygen uptake rate to monitor discovery of microbial and enzymatic biocatalysts

Dumsday

Ocal

Bridger

et al. 2008

Biotech & Bioengineering

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Arising from the requirement for discovery of novel biocatalysts with unusual properties, a process was developed which uniquely combines aspects of continuous culture with the measurement of oxygen uptake. This adaptation of the chemostat can be used to facilitate the isolation of a number of microorganisms with desirable properties, particularly those with useful metabolic capabilities and/or enzymes. The technique was also used to provide feedback on the metabolic status of a microbial population and increase the feed flow rate (i.e., dilution rate) thereby enabling the isolation of microorganisms with enhanced 1,3-propanediol dehydrogenase activity. The use of oxygen uptake as an indicator of cellular activity enables indirect measurement of substrate utilization and provides a real-time online assessment of the status of microbial enrichment or evolutionary processes and provides an opportunity, through the use of feedback systems, to control these processes. To demonstrate the utility of the technique, oxygen uptake rate (OUR) was compared with a range of conventional analytical techniques that are typically used to monitor enrichment/evolutionary processes and showed good correlation. Further validation was demonstrated by monitoring a characterizable microbial population shift using OUR. The population change was confirmed using off-line analytical techniques that are traditionally used to determine microbial activity. OUR was then used to monitor the enrichment of microorganisms capable of using a solvent (1-methyl-2-pyrrolidinone) as the sole source of carbon for energy and biomass formation from a heterogeneous microbial population. After purification the microorganisms taken from the enrichment process were able to completely utilize 1 g L(-1) 1-methyl-2-pyrrolidinone within 24 h demonstrating that the technique had correctly indicated the enriched population was capable of growth on 1-methyl-2-pyrrolidinone. The technique improves on conventional microbial enrichment that utilizes continuous culture by providing a real-time assessment of the enrichment process and the opportunity to use the OUR output for automated control and variation of one or more growth parameters.

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“…The next step is phosphorylation of the latter product, which is catalyzed by the glycolytic enzyme dihydroxyacetone kinase. Then, the phosphorylated product is subjected to glycolysis (Daniel et al, 1995;Luers et al, 1997;Macis et al, 1998;Zhu et al, 2002;da Silva et al, 2009). In the reducing pathway, glycerol is converted to 3-hydroxypropionaldehyde in reactions catalyzed by coenzyme B 12 -dependent glycerol dehydratase and related diol dehydratases.…”

Section: Metabolic Pathwaysmentioning

confidence: 99%

RESEARCH PAPER Biotechnological production of 1,3-propanediol from crude glycerol

Drożdżyńska¹,

Leja²,

Czaczyk³

2011

bta

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1,3-Propanediol (1,3-PD) is one of the important products used in chemical industry, in particular for polyesters production (e.g. polyethers and polyurethanes). Using crude glycerol for producing 1,3-PD is a good solution from the economical as well as ecological point of view. Glycerol produced by cleavage of natural fats can be microbially converted to 1.3-propanediol by, among others, Citrobacter, Klebsiella, Lactobacillus, Enterobacter, and Clostridium strains. Biotechnological production of 1,3-PD from waste biomass is a promising and attractive alternative to the traditional chemical synthesis. The production of 1,3-PD by glycerol fermentation was already reported in 1881. The microbiological bioconversion pathway of glycerol to 1,3-PD has been known for long but the microorganisms taking part in this fermentation are not efficient. In addition, they are pathogenic. Consequently, natural producers of 1,3-PD are still being sought. In this review we present a historical outline of 1,3-PD production, as well as the microorganisms and their metabolic pathways that are involved in glycerol fermentation to 1,3-PD.

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Glycerol conversion to 1,3-propanediol by Clostridium pasteurianum: cloning and expression of the gene encoding 1,3-propanediol dehydrogenase

Cited by 67 publications

References 32 publications

1,3-Propanediol Dehydrogenase fromKlebsiella pneumoniae: Decameric Quaternary Structure and Possible Subunit Cooperativity

1,3-Propanediol Dehydrogenase fromKlebsiella pneumoniae: Decameric Quaternary Structure and Possible Subunit Cooperativity

The use of oxygen uptake rate to monitor discovery of microbial and enzymatic biocatalysts

RESEARCH PAPER Biotechnological production of 1,3-propanediol from crude glycerol

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