Glycerol metabolism and its regulation in lactic acid bacteria

Doi, Yuki

doi:10.1007/s00253-019-09830-y

Cited by 74 publications

(32 citation statements)

References 96 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This reductive process, i.e., glycerol degradation III, is a dismutation reaction involving glycerol dehydration to 3-hydroxypropanal (3-HPA) by glycerol dehydratase (EC 4.2.1.30). 3-HPA is further fermented to 1,3-propanediol (PDO) by an NADH-associated 1,3-propanediol dehydrogenase (EC 1.1.1.202) [ 42 ]. The four enzymes involved in both pathways, i.e., glycerol dehydrogenase (EC 1.1.1.6), DHA kinase (EC 2.7.1.29), glycerol dehydratase (EC 4.2.1.30), and 1,3-propanediol dehydrogenase (EC 1.1.1.202) are encoded by dha regulon [ 43 ].…”

Section: Resultsmentioning

confidence: 99%

“…An additional dehydrogenation pathway for glycerol utilization (i.e., glycerol degradation V) involves the conversion of glycerol to DHA by glycerol dehydrogenase, followed by phosphorylation of DHA to DHAP by a PEP-dependent DHA kinase (EC 2.7.1.121; encoded by dhaKL ) [ 42 , 43 ]. The genes for PEP-dependent DHA kinase (EC 2.7.1.121) were exclusively identified in the APG4 genome.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Genome analysis to decipher syntrophy in the bacterial consortium ‘SCP’ for azo dye degradation

2021

View full text Add to dashboard Cite

Background A bacterial consortium SCP comprising three bacterial members, viz. Stenotrophomonas acidaminiphila APG1, Pseudomonas stutzeri APG2 and Cellulomonas sp. APG4 was developed for degradation of the mono-azo dye, Reactive Blue 28. The genomic analysis of each member of the SCP consortium was done to elucidate the catabolic potential and role of the individual organism in dye degradation. Results The genes for glycerol utilization were detected in the genomes of APG2 and APG4, which corroborated with their ability to grow on a minimal medium containing glycerol as the sole co-substrate. The genes for azoreductase were identified in the genomes of APG2 and APG4, while no such trait could be determined in APG1. In addition to co-substrate oxidation and dye reduction, several other cellular functions like chemotaxis, signal transduction, stress-tolerance, repair mechanisms, aromatic degradation, and copper tolerance associated with dye degradation were also annotated. A model for azo dye degradation is postulated, representing the predominant role of APG4 and APG2 in dye metabolism while suggesting an accessory role of APG1. Conclusions This exploratory study is the first-ever attempt to divulge the genetic basis of azo-dye co-metabolism by cross-genome comparisons and can be harnessed as an example for demonstrating microbial syntrophy.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Genome analysis to decipher syntrophy in the bacterial consortium ‘SCP’ for azo dye degradation

2021

View full text Add to dashboard Cite

show abstract

“…Glycerol was quickly consumed under all reactor conditions, except for the R5 T-pH-LowSRT experiment (Figure 2). Glycerol metabolism has been demonstrated, or its metabolic potential has been suggested, in microorganisms related to the abundant taxa observed in the bioreactors, including members of the Clostridia class and Lactobacillaceae family of Firmicutes (Veiga da Cunha and Foster, 1992;Alvarez et al, 2004;Cotta and Forster, 2006;Rainey, 2011;Gänzle, 2015;Doi, 2019;Palevich et al, 2019;Veras et al, 2019;Scarborough et al, 2020). In addition to being a coproduct of the fuel ethanol industry, glycerol is a coproduct from the biodiesel industry, which is of growing interest to researchers as a substrate for microbial fermentation of SA and LA (Litsanov et al, 2013;Gao et al, 2016;Murakami et al, 2016;Kuenz et al, 2020;Zheng et al, 2021).…”

Section: Importance Of Glycerol As a Fermentation Carbon Sourcementioning

confidence: 99%

Diverse Profile of Fermentation Byproducts From Thin Stillage

Fortney

Hanson

Rosa

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

The economy of biorefineries is influenced not only by biofuel production from carbohydrates but also by the production of valuable compounds from largely underutilized industrial residues. Currently, the demand for many chemicals that could be made in a biorefinery, such as succinic acid (SA), medium-chain fatty acids (MCFAs), and lactic acid (LA), is fulfilled using petroleum, palm oil, or pure carbohydrates as raw materials, respectively. Thin stillage (TS), the residual liquid material following distillation of ethanol, is an underutilized coproduct from the starch biofuel industry. This carbon-rich material has the potential for chemical upgrading by microorganisms. Here, we explored the formation of different fermentation products by microbial communities grown on TS using different bioreactor conditions. At the baseline operational condition (6-day retention time, pH 5.5, 35°C), we observed a mixture of MCFAs as the principal fermentation products. Operation of a bioreactor with a 1-day retention time induced an increase in SA production, and a temperature increase to 55°C resulted in the accumulation of lactic and propionic acids. In addition, a reactor operated with a 1-day retention time at 55°C conditions resulted in LA accumulation as the main fermentation product. The prominent members of the microbial community in each reactor were assessed by 16S rRNA gene amplicon sequencing and phylogenetic analysis. Under all operating conditions, members of the Lactobacillaceae family within Firmicutes and the Acetobacteraceae family within Proteobacteria were ubiquitous. Members of the Prevotellaceae family within Bacteroidetes and Lachnospiraceae family within the Clostridiales order of Firmicutes were mostly abundant at 35°C and not abundant in the microbial communities of the TS reactors incubated at 55°C. The ability to adjust bioreactor operating conditions to select for microbial communities with different fermentation product profiles offers new strategies to explore and compare potentially valuable fermentation products from TS and allows industries the flexibility to adapt and switch chemical production based on market prices and demands.

show abstract

“…An important pathway for gram-positive and lactic acid bacteria, like some enterococci, is glycerol metabolism to synthetize phospholipids 62,66 . Most enterococci species, including E. faecalis and E. faecium, are able to ferment glycerol to give lactate as the final product under aerobic and microaerophilic conditions 64 .…”

Section: Nutritional Requirementsmentioning

confidence: 99%

“…Figure66. Gene complementation to verify function of the mutants ∆manX::gen and ∆murQ::gen in vitro.…”

mentioning

confidence: 99%

Characterization of Genes and Functions Required by Multidrug-resistant Enterococci to Colonize the Intestine

Duro¹

View full text Add to dashboard Cite

through nutrient depletion, specifically by reducing the levels of fructose. Finally, in vivo RNA-Seq analysis of each bacterial isolate of the consortium in combination with ex vivo and in vivo assays demonstrated that a single bacterium (Olsenella sp.) could recapitulate the protective effect.Altogether, the results obtained have identified the function of specific genes required by VRE to colonize the gut. In addition, we have identified a specific mechanism by which the microbiota confers protection against VRE colonization. These results could lead to novel therapeutic approaches to prevent infections caused by this multidrug-resistant pathogen.ARN-Seq in vivo de cada aislado en combinación con los ensayos ex vivo e in vivo demostraron que una sola bacteria (Olsenella sp.) proporciona protección.En conjunto, los resultados obtenidos han identificado la función de genes específicos requeridos por ERV para colonizar el intestino. Además, hemos identificado un mecanismo mediante el cual la microbiota confiere protección. Estos resultados podrían conducir a nuevos enfoques terapéuticos para prevenir las infecciones causadas por este patógeno multiresistente a los antibióticos.En conjunt, els resultats obtinguts han identificat la funció de gens específics requerits per ERV per a colonitzar l'intestí. A més, hem identificat un mecanisme mitjançant el qual la microbiota confereix protecció. Aquests resultats podrien conduir a nous enfocaments terapèutics per a previndre les infeccions causades per aquest patogen multiresistent als antibiòtics.

show abstract

Glycerol metabolism and its regulation in lactic acid bacteria

Cited by 74 publications

References 96 publications

Genome analysis to decipher syntrophy in the bacterial consortium ‘SCP’ for azo dye degradation

Genome analysis to decipher syntrophy in the bacterial consortium ‘SCP’ for azo dye degradation

Diverse Profile of Fermentation Byproducts From Thin Stillage

Characterization of Genes and Functions Required by Multidrug-resistant Enterococci to Colonize the Intestine

Contact Info

Product

Resources

About