Glycerol‐Releasing Activity of Histamine‐Sensitizing Factor of Bordetella pertussis for Rat Adipocytes In Vitro

Abstract

Histamine-sensitizing factor (HSF) purified from Bordetella pertussis induced specifically the release of glycerol from rat epididymal adipocytes in vitro.

In Vitro Induction of Altered Response of Rat Atria to Acetylcholine with Histamine‐Sensitizing Factor of Bordetella pertussis

“…The rabbits were bled one week after the last booster dose. The serum was capable of neutralizing 64 flg of HSF per ml by the method previously described (2).…”

“…Purified HSF, with a molecular weight of 83,000 estimated by the Sephadex G-150 column method, was prepared from B. pertussis strain 3779B as previously described (2). In ICR mice hypersensitivity to histamine was induced at a dose of I ng of HSF per mouse, and leukocytosis at 40 to 60 ng, by the method of Munoz and Bergman (4).…”

“…Histamine-sensitizing factor (HSF), a protein toxin of Bordetella pertussis which is also called leukocytosis-promoting factor (LPF), pertussis toxin or pertussigen, has been shown to induce in laboratory animals various biological reactions such as increased susceptibility to histamine as well as a number of reagents, lymphocytosis and leukocytosis, increased secretion of insulin, metabolic changes, alteration of immune response, and increased release of glycerol from adipocytes (2)(3)(4)(5). In addition, we recently.…”

Cytopathic Effect of Heat‐Labile Toxin of Bordetella parapertussis on Aortic Smooth Muscle Cells from Pigs or Guinea Pigs

“…Effects on antibody-forming cells were examined by the Jerne plaque assay (8), using sheep red cell antigen (T-dependent) and trinitrophenyl-Brucella abortus antigen (T-independent). Lipolysis was determined by the method described previously (6).…”

“…HLT purified from B. parapertussis, strain 23054, by the method previously described (3,4,6), was used. It showed 0.8 ng of the minimum dermonecrotizing dose (MND) in the shaved back of guinea pigs, similar molecular weight and subunit structure, and vasocontractive activities on peripheral vessels, to those of B. bronchiseptica-HLT described previously (3).…”