Glycerolipid and Fatty Acid Changes in Eastern White Pine Chloroplast Lamellae during the Onset of Winter

DeYoe, David R.; Brown, Gene M.

doi:10.1104/pp.64.6.924

Cited by 38 publications

(15 citation statements)

References 19 publications

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“…After the thaw, both mCa and freezing tolerance returned to prethaw levels. Sequential changes in mCa and frost tolerance levels, coupled with the well documented changes in membrane structure in leaf tissue of north temperate conifers during cold acclimation (e.g., DeYoe and Brown, 1979;Beck, 1982, 1984), strongly support the contention that mCa is important to cold tolerance and that acid mist alterations of mCa cause membrane destabilization and a concomitant loss in freezing tolerance.…”

Section: Evidence Of Acid-induced Calcium and Cold Tolerance Perturbamentioning

confidence: 70%

Physiological and Environmental Causes of Freezing Injury in Red Spruce

Schaberg¹,

DeHayes²

2000

Ecological Studies

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Section: Evidence Of Acid-induced Calcium and Cold Tolerance Perturbamentioning

confidence: 70%

Physiological and Environmental Causes of Freezing Injury in Red Spruce

Schaberg¹,

DeHayes²

2000

Ecological Studies

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“…Bervaes et al (2) have also observed that hardened pine has a lower MGDG/DGDG ratio than dehardened pine. Such a change would increase the capacity of the polar heads of the galactolipids to bind water, which might be an acclimation of the thylakoids to increase their resistance to freeze dehydration when water freezes extracellularly during the winter (2,4).…”

Section: Discussionmentioning

confidence: 99%

Seasonally Induced Changes in Acyl Lipids and Fatty Acids of Chloroplast Thylakoids of Pinus silvestris

Öquist¹

1982

Plant Physiol.

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Current-year needles were sampled regularly from an approximately 20-year-old natural stand of Pinus silvestris. Chloroplast thylakoids were isolated. The electron transport capacities of photosystem II + photosystem I, as well as of the partial photoreactions, were measured. The amounts and the fatty acid compositions of monogalactosyldiglyceride, digalactosyldiglyceride, and sulfolipid of the thylakoids were analyzed. The fatty acid composition of the phospholipids (total) was also determined.There was a 2-fold increase in the content of the galactolipids and the sulfolipid in the thylakoid preparations during the autumn. Except for monogalactosyldiglyceride that decreased in content during early winter, the content of the other two lipids remained high during the winter, unti spring when their content fell toward a low summer level. The ratio of monogalactosyldiglyceride to digalactosyldiglyceride was lower in thylakoids isolated from frosthardened (autumn, winter) than in those isolated from unhardened (summer) needles.Only monogalactosyldiglyceride showed pronounced and significant seasonal variations in the molar ratio of unsaturated to saturated fatty acids. It was low in the winter, and it increased strongly during the spring. The highest ratio was observed in September, prior to the first night frosts, after which a steady decrease toward the low winter value occurred. This decrease is thought to be a reflection of membrane damages (photooxidation of membrane components, including polyunsaturated fatty acids), as earlier seen reflected in chlorophyll destruction, electron transport inhibition, and structural changes.There was very good correlation between the seasonal variations in the capacity for electron transport from water to NADP and the level of unsaturation of monogalactosyldiglyceride (r = 0.93). It has been shown earlier that the winter inhibition of electron transport preferentially occurs at the lipophilic electron transport carrier plastoquinone and that the free miscibility of plastoquinone and monogalactosyldiglyceride is prevented in saturated monogalactosyldiglyceride. Therefore, it is hypothesized that the decreased level of unsaturation of monogalactosyldiglyceride reduces the mobility of plastoquinone in the thylakoids, resulting in an inhibition of the electron transport by way of plastoquinone.We have published a series of papers on climatically induced changes of the function and organization of the chloroplast thylakoids of Pinus silvestris. The studies recently have been reviewed (17). Prolonged exposure of conifers to near-and sub-zero temperatures causes a well-known depression of the rate of net ' Supported by the Swedish Natural Science Research Council. photosynthesis (22). Studies of pine seedlings exposed to artificial frosthardening (photoperiod, 8 h; 3°C) followed by winter stress in light at sub-zero temperature (photoperiod, 8 h; -5°C) showed that the decay and final winter inhibition of net photosynthesis was paralleled with an inhibition of the photosynthet...

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“…The resistance to cold in general greatly depends on the presence of sufficient amounts of unsaturated lipids. A corresponding increase of a-linolenic acid and galactolipids during autumn or winter has been observed in needles of pine (SENSER 1982), Norway spruce (OEQUIST 1982) and Eastern white pine (DEYOE and BROWN 1979). The chances in the fatty acid pattern are accompanied by increased amounts of protein in both the younger and older needles and, in the younger needles, by slightly reduced amounts of chlorophyll b and increased amounts of carotenoids.…”

Section: Discussionmentioning

confidence: 89%