Glycerolipid transfer for the building of membranes in plant cells

Jouhet, Juliette; Maréchal, Éric; Block, Maryse A.

doi:10.1016/j.plipres.2006.06.002

Cited by 134 publications

(90 citation statements)

References 105 publications

(112 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In plants and algae, MGDG and DGDG are synthesized within plastid envelope membranes (29,31,(54)(55)(56)(57)(58). In plants, MGDG is produced by the galactosylation of DAG, whereas it is generated by a two-step glucosylation and epimerization in cyanobacteria (59).…”

Section: Discussionmentioning

confidence: 99%

Subcellular localization and dynamics of a digalactolipid-like epitope in Toxoplasma gondii

Botté

Saïdani

Mondragón

et al. 2008

Journal of Lipid Research

Self Cite

View full text Add to dashboard Cite

Toxoplasma gondii is a unicellular parasite characterized by unique extracellular and intracellular membrane compartments. The lipid composition of subcellular membranes has not been determined, limiting our understanding of lipid homeostasis, control, and trafficking, a series of processes involved in pathogenesis. In addition to a mitochondrion, Toxoplasma contains a plastid called the apicoplast. The occurrence of a plastid raised the question of the presence of chloroplast galactolipids. Using three independent rabbit and rat antibodies against digalactosyldiacylglycerol (DGDG) from plant chloroplasts, we detected a class of Toxoplasma lipids harboring a digalactolipid-like epitope (DGLE). Immunolabeling characterization supports the notion that the DGLE polar head is similar to that of DGDG. Mass spectrometry analyses indicated that dihexosyl lipids having various hydrophobic moieties (ceramide, diacylglycerol, and acylalkylglycerol) might react with anti-DGDG, but we cannot exclude the possibility that more complex dihexosyl-terminated lipids might also be immunolabeled. DGLE localization was analyzed by immunofluorescence and immunoelectron microscopy and confirmed by subcellular fractionation. No immunolabeling of the apicoplast could be observed. DGLE was scattered in pellicle membrane domains in extracellular tachyzoites and was relocalized to the anterior tip of the cell upon invasion in an actin-dependent manner, providing insights on a possible role in pathogenetic processes. DGLE was detected in other Apicomplexa (i.e

show abstract

Section: Discussionmentioning

confidence: 99%

Subcellular localization and dynamics of a digalactolipid-like epitope in Toxoplasma gondii

Botté

Saïdani

Mondragón

et al. 2008

Journal of Lipid Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…The concept of glycerolipid transfer via interorganelle contact site is not new; indeed, the ER-plastid contact site has been observed with a microscope using optical tweezers (Andersson et al, 2007). This subject has been intensively reviewed by Jouhet et al (2007). The central role of the ER-plastid junction in cellular lipid metabolism has previously been shown in other studies on TAG biosynthesis in C. reinhardtii (Fan et al, 2011;Goodson et al, 2011).…”

Section: Possible Mechanisms For V-3 Fatty Acid Distribution Into Plamentioning

confidence: 95%

The Green Microalga Chlamydomonas reinhardtii Has a Single -3 Fatty Acid Desaturase That Localizes to the Chloroplast and Impacts Both Plastidic and Extraplastidic Membrane Lipids

et al. 2013

View full text Add to dashboard Cite

The v-3 polyunsaturated fatty acids account for more than 50% of total fatty acids in the green microalga Chlamydomonas reinhardtii, where they are present in both plastidic and extraplastidic membranes. In an effort to elucidate the lipid desaturation pathways in this model alga, a mutant with more than 65% reduction in total v-3 fatty acids was isolated by screening an insertional mutant library using gas chromatography-based analysis of total fatty acids of cell pellets. Molecular genetics analyses revealed the insertion of a TOC1 transposon 113 bp upstream of the ATG start codon of a putative v-3 desaturase (CrFAD7; locus Cre01.g038600). Nuclear genetic complementation of crfad7 using genomic DNA containing CrFAD7 restored the wild-type fatty acid profile. Under standard growth conditions, the mutant is indistinguishable from the wild type except for the fatty acid difference, but when exposed to short-term heat stress, its photosynthesis activity is more thermotolerant than the wild type. A comparative lipidomic analysis of the crfad7 mutant and the wild type revealed reductions in all v-3 fatty acid-containing plastidic and extraplastidic glycerolipid molecular species. CrFAD7 was localized to the plastid by immunofluorescence in situ hybridization. Transformation of the crfad7 plastidial genome with a codon-optimized CrFAD7 restored the v-3 fatty acid content of both plastidic and extraplastidic lipids. These results show that CrFAD7 is the only v-3 fatty acid desaturase expressed in C. reinhardtii, and we discuss possible mechanisms of how a plastid-located desaturase may impact the v-3 fatty acid content of extraplastidic lipids.

show abstract

“…The photosynthetic machinery is housed within a membrane that contains monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) [reviewed by Jones (2007) and Jouhet et al (2007)]. While the fatty acid composition of MGDG-and DGDGcontaining lipid fractions has been determined for several dinoflagellates (Leblond & Chapman, 2000;Leblond et al, 2006), our knowledge of dinoflagellate lipid biochemistry is extremely limited in some respects.…”

Section: Introductionmentioning

confidence: 99%

Mono- and digalactosyldiacylglycerol composition of dinoflagellates. I. Peridinin-containing taxa

Gray¹,

Lasiter²,

Leblond³

2009

European Journal of Phycology

108

View full text Add to dashboard Cite

Two glycolipids, monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), are components of chloroplast membranes. This study addresses the lack of information regarding the major intact forms of these glycolipids in photosynthetic dinoflagellates through the use of a full-scan positive-ion electrospray ionization/mass spectrometry (ESI/ MS) survey of MGDG and DGDG in 35 peridinin-containing taxa from the class Dinophyceae. Further analysis using positive-ion electrospray ionization/mass spectrometry/mass spectrometry (ESI/MS/MS) was performed to determine the positional distribution of fatty acids associated with MGDG and DGDG. The dinoflagellates examined were divided into two clusters based on the forms of MGDG and DGDG present. The first possessed 18:5/18:4 MGDG (sn

show abstract

Glycerolipid transfer for the building of membranes in plant cells

Cited by 134 publications

References 105 publications

Subcellular localization and dynamics of a digalactolipid-like epitope in Toxoplasma gondii

Subcellular localization and dynamics of a digalactolipid-like epitope in Toxoplasma gondii

The Green Microalga Chlamydomonas reinhardtii Has a Single -3 Fatty Acid Desaturase That Localizes to the Chloroplast and Impacts Both Plastidic and Extraplastidic Membrane Lipids

Mono- and digalactosyldiacylglycerol composition of dinoflagellates. I. Peridinin-containing taxa

Contact Info

Product

Resources

About