Glycerophospholipid synthesis and functions in Pseudomonas

Kondakova, Tatiana; D’Heygere, F; Feuilloley, Marc; Orange, Nicole; Heipieper, Hermann J.; Poc, Cécile Duclairoir

doi:10.1016/j.chemphyslip.2015.06.006

Cited by 44 publications

(30 citation statements)

References 186 publications

(247 reference statements)

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“…The fact that 13-HPOT does not penetrate deeply into the bilayer should render it an inducer of positive spontaneous curvature and, in turn, favours its packing with a negative spontaneous curvature lipid such as PE. It would probably improve packing and limit disordering compared to a more positive-curvature lipid, which is in accordance with the absence of leakage observed in our permeabilization assays [72,73]. This specific and strong lipid interaction can lead to significant changes in the organization of the membrane, and in the distribution and functioning of some membrane proteins [74].…”

Section: General Discussion and Conclusionsupporting

confidence: 85%

Linolenic fatty acid hydroperoxide acts as biocide on plant pathogenic bacteria: Biophysical investigation of the mode of action

Deboever

Lins

Ongena

et al. 2020

Bioorganic Chemistry

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Fatty acid hydroperoxides (HPO) are free phyto-oxylipins known for their crucial role as signalling molecules during plant defense mechanisms. They were also demonstrated to have direct biocidal activities against plant pathogens including gram negative bacteria. In the present work, the biocidal effect of one linolenic fatty acid hydroperoxide, namely 13-HPOT has been investigated on three plant pathogen gram negative bacteria: Pectobacterium carotovorum, Pseudomonas syringae and Xanthomonas translucens. We showed that 13-HPOT has a strong dose response effect on those phytopathogens. In a second part, the molecular mechanism behind the antibacterial effect of 13-HPOT was investigated at a molecular level using an integrative biophysical approach combining in vitro and in silico methods. Since other antimicrobial amphiphilic molecules have been shown to target the lipid membrane of the organisms they act on, we focused our study on the interaction of 13-HPOT with biomimetic membranes. In a first step, we hypothesized that the inner membrane of the bacteria was the main site of action of 13-HPOT and hence we used lipids representative of this membrane to form our models. Our results indicated that 13-HPOT can interact with the lipid representative of the inner bacterial plasma membrane. A strong membrane insertion is suggested but no major permeabilization of the membrane is observed. Phosphatidylethanolamine (PE) and cardiolipin (CL), present in the bacterial plasma membrane, appear to play important roles in this interaction. We suggest that the mode of action of 13-HPOT should involve either subtle changes in membrane properties, such as its lateral organization and distribution, and/or interactions with membrane proteins.

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Section: General Discussion and Conclusionsupporting

confidence: 85%

Linolenic fatty acid hydroperoxide acts as biocide on plant pathogenic bacteria: Biophysical investigation of the mode of action

Deboever

Lins

Ongena

et al. 2020

Bioorganic Chemistry

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“…PCC6803 DesC (39% identity) and an additional one is related to P. aeruginosa DesA (47% identity). Both are acyl-lipid desaturases that incorporate double bonds in membrane phospholipids ( Murata and Wada, 1995 ; Kondakova et al, 2015 ). P. salmonis lacks the FabA-FabB system for the synthesis of unsaturated fatty acids, making desaturases a requirement for their production.…”

Section: Resultsmentioning

confidence: 99%

Analysis of Piscirickettsia salmonis Metabolism Using Genome-Scale Reconstruction, Modeling, and Testing

et al. 2017

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Piscirickettsia salmonis is an intracellular bacterial fish pathogen that causes piscirickettsiosis, a disease with highly adverse impact in the Chilean salmon farming industry. The development of effective treatment and control methods for piscireckttsiosis is still a challenge. To meet it the number of studies on P. salmonis has grown in the last couple of years but many aspects of the pathogen’s biology are still poorly understood. Studies on its metabolism are scarce and only recently a metabolic model for reference strain LF-89 was developed. We present a new genome-scale model for P. salmonis LF-89 with more than twice as many genes as in the previous model and incorporating specific elements of the fish pathogen metabolism. Comparative analysis with models of different bacterial pathogens revealed a lower flexibility in P. salmonis metabolic network. Through constraint-based analysis, we determined essential metabolites required for its growth and showed that it can benefit from different carbon sources tested experimentally in new defined media. We also built an additional model for strain A1-15972, and together with an analysis of P. salmonis pangenome, we identified metabolic features that differentiate two main species clades. Both models constitute a knowledge-base for P. salmonis metabolism and can be used to guide the efficient culture of the pathogen and the identification of specific drug targets.

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“…Secondly, lipids are important inflammatory mediators during infection, and changes in lipid metabolites are observed during conditions such as sepsis, bacteremia, and viral infection (Cui, et al, 2013;Langley, et al, 2013;. Thirdly, lipids are important in the structure and function of bacteria, and lipid metabolites are produced by bacteria (Kondakova, et al, 2015;. Therefore, in future studies for biomarker discovery, it would be worthwhile to optimize the extraction and detection steps for finding lipid metabolites.…”

Section: Discussionmentioning

confidence: 99%

Lipid metabolites as potential diagnostic and prognostic biomarkers for acute community acquired pneumonia

Lee

Wong

et al. 2016

Diagnostic Microbiology and Infectious Disease

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Early diagnosis of acute community-acquired pneumonia (CAP) is important in patient triage and treatment decisions. To identify biomarkers that distinguish patients with CAP from non-CAP controls, we conducted an untargeted global metabolome analysis for plasma samples from 142 patients with CAP (CAP cases) and 97 without CAP (non-CAP controls). Thirteen lipid metabolites could discriminate between CAP cases and non-CAP controls with area-under-the-receiver-operating-characteristic curve of >0.8 (P ≤ 10(-9)). The levels of glycosphingolipids, sphingomyelins, lysophosphatidylcholines and L-palmitoylcarnitine were higher, while the levels of lysophosphatidylethanolamines were lower in the CAP cases than those in non-CAP controls. All 13 metabolites could distinguish CAP cases from the non-infection, extrapulmonary infection and non-CAP respiratory tract infection subgroups. The levels of trihexosylceramide (d18:1/16:0) were higher, while the levels of lysophosphatidylethanolamines were lower, in the fatal than those of non-fatal CAP cases. Our findings suggest that lipid metabolites are potential diagnostic and prognostic biomarkers for CAP.

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Glycerophospholipid synthesis and functions in Pseudomonas

Cited by 44 publications

References 186 publications

Linolenic fatty acid hydroperoxide acts as biocide on plant pathogenic bacteria: Biophysical investigation of the mode of action

Linolenic fatty acid hydroperoxide acts as biocide on plant pathogenic bacteria: Biophysical investigation of the mode of action

Analysis of Piscirickettsia salmonis Metabolism Using Genome-Scale Reconstruction, Modeling, and Testing

Lipid metabolites as potential diagnostic and prognostic biomarkers for acute community acquired pneumonia

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