Glycin-rich antimicrobial peptide YD1 from B. amyloliquefaciens, induced morphological alteration in and showed affinity for plasmid DNA of E. coli

Abstract

Antimicrobial peptides (AMPs), low-molecular-weight proteins with broad-spectrum antimicrobial activity, are the most promising candidates for the development of novel antimicrobials. A powerful cationic glycine-rich AMP YD1 (MW ~ 1.0 kDa) was purified from Bacillus amyloliquefaciens CBSYD1 isolated from traditional Korean fermented food kimchi, for the treatment of multidrug-resistant (MDR) bacteria. Strain CBSYD1 was identified 99.79% similar to Bacillus amyloliquefaciens subsp. plantarum FZB42(T) by 16S rRNA sequence analysis. The amino acid sequence residues of YD1 were determined to be Ala-Pro-Lys-Gly-Val-Gln-Gly-Pro-Asn-Gly by Edman degradation method. After the analysis and comparison of YD1 peptide sequence using several bioinformatic servers, peptide sequence has been considered to be unique. YD1 displayed antimicrobial activity against gram-positive and gram-negative bacteria. The minimal inhibitory concentrations (MIC) of YD1 for Escherichia coli KCTC1923 (E. coli), methicillin-resistant Staphylococcus aureus B15 (MRSA), and vancomycin-resistant enterococci (VRE) ranged from 8 to 64 µg/mL, representing greater potency than commercial reference antibiotics. The antimicrobial mechanism of YD1 was determined to involve cell-penetrating translocation inside the cell and interaction with the DNA leading ultimately to bacterial cell death. Analogously, Gly-Pro-Asn-Gly is the likely expected cell-penetrating motif for YD1. YD1 could be a promising antimicrobial agent for the clinical application.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-016-0315-8) contains supplementary material, which is available to authorized users.

Reduction of Oxidative Stress through Activating the Nrf2 mediated HO-1 Antioxidant Efficacy Signaling Pathway by MS15, an Antimicrobial Peptide from Bacillus velezensis

“…Peptide MS15 imposed cyclic structure so that the amino acid sequence could not be detected by using the Edman degradation method, although purification was detected with MALDI TOF/MS. The peptide was purified by two-step column chromatography with the 24.49-fold and recovered activity of 5.92%, that of total bacteriocin activity was found at 16000 AU, which was consistent with our previous report [ 18 , 22 ]. Primarily, we investigated the antimicrobial spectrum of peptide MS15 against different pathogens that were significantly higher than our previous report [ 37 ].…”

“…Antimicrobial activity was established by the method of disc diffusion. Briefly, paper disc filter (8 mm, Roshi Kaisha, Tokyo, Japan) soaked on the face of the petri dish with AMP (40 µL) was positioned, including the MH agar plate, overlay with Mycobacterium smegmatis [ 18 ]. Optimization of production culture media has enormous implications in the enhanced culture of AMP.…”

Identification and characterization of proteins in the Amblyomma americanum tick cement cone

“…The observation that some of the transcripts were highly expressed throughout the 5 day tick feeding period tested here, could indicate that candidate GRP genes were associated with other functions of tick feeding physiology. Whereas in tick research GRPs are mostly known for their potential roles in tick cement formation (Kemp et al., 1982), indirect evidence in other organisms suggests that these proteins could be involved in a myriad of other functions including anti-microbial activity (Alekseev et al, 1995; Carlier et al, 2015; Rahman et al, 2017), innate immunity in mammals (Tam et al, 2012), wound healing, plant defense and stress responses (Mangeon et al, 2010; Lin and Chen, 2014).…”

The Interfacial Interactions of Glycine and Short Glycine Peptides in Model Membrane Systems

“…Having only a hydrogen atom as its substituent, it is the only amino acid that is achiral, and as such, it is compatible with hydrophilic environments, and although it is not directly soluble in for example isooctane ( Figure S1 ) it can partition toward hydrophobic regions in inhomogenous environments. In addition, it has many biological functions, one of the most notable of which as a simple inhibitory and excitatory neurotransmitter, and as such, it is a logical representative amino acid for investigation of simple peptide and amino acid interactions with a membrane, and in addition, there have been numerous reports of glycine-rich AMPs [ 25 , 26 , 27 , 28 ].…”