Glycine attenuates cerebral ischemia/reperfusion injury by inhibiting neuronal apoptosis in mice

Lü, Yan; Zhang, Jing; Ma, Bingqing; Li, Kexue; Li, Xiaoyü; Bai, Hui; Yang, Qing; Zhu, Xudong; Ben, Jingjing; Chen, Qi

doi:10.1016/j.neuint.2012.07.005

Cited by 38 publications

(24 citation statements)

References 51 publications

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“…Although no direct link has been established between osmotic stress and oocyte apoptosis, cell death through apoptosis were also happened after oocyte vitrification495051. Glycine prevents the cells against apoptosis has been reported in rat endothelial cells52, mice neuronal cells53 and chick embryos54. For mouse oocytes, we demonstrated for the first time that the glycine supplementation during vitrification/thawing could inhibit early apoptosis (detected by Annexin V binding) in vitrified GV oocytes.…”

Section: Discussionmentioning

confidence: 76%

Glycine increases preimplantation development of mouse oocytes following vitrification at the germinal vesicle stage

Cao

Rose

Wang

et al. 2016

Sci Rep

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Ice-free cryopreservation, referred to as vitrification, is receiving increased attention in the human and animal assisted reproduction. However, it introduces the detrimental osmotic stress by adding and removing high contents of cryoprotectants. In this study, we evaluated the effects of normalizing cell volume regulation by adding glycine, an organic osmolyte, during vitrification of mouse germinal vesicle stage oocyte and/or subsequent maturation on its development. The data showed that glycine supplementation in either vitrification/thawing or maturation medium significantly improved the cytoplasmic maturation of MII oocytes manifested by spindle assembly, chromosomal alignment, mitochondrial distribution, euploidy rate, and blastocyst development following fertilization in vitro, compared to the control without glycine treatment. Furthermore, glycine addition during both vitrification/thawing and maturation further enhanced the oocyte quality demonstrated by various markers, including ATP contents and embryo development. Lastly, the effect of anti-apoptosis was also observed when glycine was added during vitrification. Our result suggests that reducing osmotic stress induced by vitrification could improve the development of vitrified mouse oocyte.

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Section: Discussionmentioning

confidence: 76%

Glycine increases preimplantation development of mouse oocytes following vitrification at the germinal vesicle stage

Cao

Rose

Wang

et al. 2016

Sci Rep

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“…The OGD/R injury model using SH-SY5Y cell to mimic the process of neuronal cell death in cerebral hypoxia and ischemia is widely applied to explore the probable mechanism in neuroprotection, especially in the field of cerebral infarction (Chan et al, 2015;Lu et al, 2012;Shi et al, 2012). At present, MTT and CCK8 assay are two main methods to measure the cell viability Zhou et al, 2016), which is a good parameter to reflect the extent of cell injury.…”

Section: Discussionmentioning

confidence: 99%

Panax notoginseng saponins provide neuroprotection by regulating NgR1/RhoA/ROCK2 pathway expression, in vitro and in vivo

Shi

Yang

et al. 2016

Journal of Ethnopharmacology

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“…Since there are multiple experimental models reviewed in the preceding sections where ERK1/2 and AKT activation cannot occur and/or cannot explain the timing of glycine’s effects relative to injury, those pathways cannot be an essential or generalized feature of glycine cytoprotection. These authors have also reported strong glycine cytoprotection of primary neuron cultures subjected to oxygen and glucose deprivation (OGD) that is convincingly blocked by anti-GlyR and antibodies or GlyRα 1 knockdown [157]. Injury in that system was also strongly decreased by the NMDA receptor antagonist, MK-801, although its effect was independent of GlyR.…”

Section: Relationship Of Cytoprotection To Neuronal Glycine Receptorsmentioning

confidence: 99%

The role of glycine in regulated cell death

Weinberg

Bienholz

Venkatachalam

2016

Cell. Mol. Life Sci.

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The cytoprotective effects of glycine against cell death have been recognized for over 28 years. They are expressed in multiple cell types and injury settings that lead to necrosis, but are still not widely appreciated or considered in the conceptualization of cell death pathways. In this paper we review the available data on the expression of this phenomenon, its relationship to major pathophysiologic pathways that lead to cell death and immunomodulatory effects, the hypothesis that it involves suppression by glycine of the development of a hydrophilic death channel of molecular dimensions in the plasma membrane, and evidence for its impact on disease processes in vivo.

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Glycine attenuates cerebral ischemia/reperfusion injury by inhibiting neuronal apoptosis in mice

Cited by 38 publications

References 51 publications

Glycine increases preimplantation development of mouse oocytes following vitrification at the germinal vesicle stage

Glycine increases preimplantation development of mouse oocytes following vitrification at the germinal vesicle stage

Panax notoginseng saponins provide neuroprotection by regulating NgR1/RhoA/ROCK2 pathway expression, in vitro and in vivo

The role of glycine in regulated cell death

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