Glycine-modified polyamidoamine dendrimers: synthesis and structural characterization using nuclear magnetic resonance, ion-mobility mass spectrometry and capillary electrophoresis

Leriche, Emma-Dune; Afonso, Carlos; Lange, Catherine; Grossel, Martin C.; Truong, Lina; Coadou, Gaël; Oulyadi, Hassan; Loutelier‐Bourhis, Corinne

doi:10.1039/c3ra43939a

Cited by 8 publications

(11 citation statements)

References 30 publications

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“…221–225 Several recent polymer studies have focused on reporting CCS values for dendrimers. 226–228 , and polymeric supermolecular assemblies utilized in molecular sensing, catalysis, and advanced materials applications. 229–237 Overall, synthetic polymers comprise about 1% of the total number of CCS values reported to date, virtually all of which are measured in helium drift gas or are calibrated to helium-equivalent values.…”

Section: Ccs Coverage Over Timementioning

confidence: 99%

Ion Mobility Collision Cross Section Compendium

2016

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In this review, we focus on an important aspect of ion mobility (IM) research, namely the reporting of quantitative ion mobility measurements in the form of the gas-phase collision cross section (CCS), which has provided a common basis for comparison across different instrument platforms and offers a unique form of structural information, namely size and shape preferences of analytes in the absence of bulk solvent. This review surveys the over 24,000 CCS values reported from IM methods spanning the era between 1975 to 2015, which provides both a historical and analytical context for the contributions made thus far, as well as insight into the future directions that quantitative ion mobility measurements will have in the analytical sciences. The analysis was conducted in 2016, so CCS values reported in that year are purposely omitted. In another few years, a review of this scope will be intractable, as the number of CCS values which will be reported in the next three to five years is expected to exceed the total amount currently published in the literature.

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Section: Ccs Coverage Over Timementioning

confidence: 99%

Ion Mobility Collision Cross Section Compendium

2016

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“…Not surprisingly, DTIMS-MS and CE also share common applications and are recognized as orthogonal to LC (103). Despite these commonalities, only a few cases of CE and DTIMS-MS as complementary or comparative techniques exist (104, 105). The first attempt to couple CE and DTIMS was performed by the Hill group in 1989 (106) with their newly developed ESI source for the IMS instrument (107).…”

Section: Capillary Electrophoresis–drift Tube Ion Mobility Spectrometmentioning

confidence: 99%

Coupling Front-End Separations, Ion Mobility Spectrometry, and Mass Spectrometry For Enhanced Multidimensional Biological and Environmental Analyses

Zheng

Wojcik

Zhang

et al. 2017

Annual Rev. Anal. Chem.

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Ion mobility spectrometry (IMS) is a widely used analytical technique for rapid molecular separations in the gas phase. Though IMS alone is useful, its coupling with mass spectrometry (MS) and front-end separations is extremely beneficial for increasing measurement sensitivity, peak capacity of complex mixtures, and the scope of molecular information available from biological and environmental sample analyses. In fact, multiple disease screening and environmental evaluations have illustrated that the IMS-based multidimensional separations extract information that cannot be acquired with each technique individually. This review highlights three-dimensional separations using IMS-MS in conjunction with a range of front-end techniques, such as gas chromatography, supercritical fluid chromatography, liquid chromatography, solid-phase extractions, capillary electrophoresis, field asymmetric ion mobility spectrometry, and microfluidic devices. The origination, current state, various applications, and future capabilities of these multidimensional approaches are described in detail to provide insight into their uses and benefits.

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“…Ideal nanocarriers are required to retain and selectively release the drug specifically in tumor cells and need to be compatible with the immune system and exhibit a long lifespan in the circulation [7,16]. A comprehensive range of nanocarriers with different nanostructures have been fabricated to enhance the therapeutic efficiency of gemcitabine, including polymersomes [17], liposomes [18], micelles [14,19], and polymeric nanoparticles, including dendrimers [15,20,21].…”

Section: Introductionmentioning

confidence: 99%

“…The uniformity and hyper-branched characteristics of PAMAM enable the dendrimer to cross the cell membrane of cancer cells and carry a high cargo content [27]. The low pKa value of the PAMAM dendrimer reduces nonspecific binding of blood proteins and enables sustained release of the drug through the protonation of dendrimer [21]. Several reports have demonstrated the core and void space of PAMAM dendrimer can encapsulate chemotherapeutic drugs and mediate excellent cellular drug delivery [20].…”

Section: Introductionmentioning

confidence: 99%

Biotin-Decorated PAMAM G4.5 Dendrimer Nanoparticles to Enhance the Delivery, Anti-Proliferative, and Apoptotic Effects of Chemotherapeutic Drug in Cancer Cells

et al. 2020

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Biotin receptors are overexpressed by various types of solid cancer cells and play a significant role in tumor metabolism, growth, and metastasis. Thus, targeting the biotin receptors on tumor cells may enhance the efficiency and reduce the side-effects of chemotherapy. The aim of this study was to develop a biotin-coupled poly(amido)amine (PAMAM) (PG4.5) dendrimer nanoparticle to enhance the tumor-specific delivery and intracellular uptake of anticancer drugs via receptor-mediated endocytosis. We modified PG4.5 with diethylenetriamine (DETA) followed by biotin via an amide bond and characterized the resulting PG4.5-DETA-biotin nanoparticles by 1H NMR, FTIR, and Raman spectroscopy. Loading and releasing of gemcitabine (GEM) from PG4.5-DETA-biotin were evaluated by UV–Visible spectrophotometry. Cell viability and cellular uptake were examined by MTT assay and flow cytometry to assess the biocompatibility, cellular internalization efficiency and antiproliferative activity of PG4.5-DETA-biotin/GEM. Gemcitabine-loaded PG4.5-DETA-biotin nanoparticles were spherical with a particle size of 81.6 ± 6.08 nm and zeta potential of 0.47 ± 1.25 mV. Maximum drug-loading content and encapsulation efficiency were 10.84 ± 0.16% and 47.01 ± 0.71%, respectively. Nearly 60.54 ± 1.99% and 73.96 ± 1.14% of gemcitabine was released from PG4.5-DETA-biotin/GEM nanoparticles after 48 h at the acidic pH values of 6.5 and 5, respectively. Flow cytometry and fluorescence microscopy of cellular uptake results revealed PG4.5-DETA-biotin/GEM nanoparticles selectively targeted cancer cells in vitro. Cytotoxicity assays demonstrated gemcitabine-loaded PG4.5-DETA-biotin significantly reduced cell viability and induced apoptosis in HeLa cells. Thus, biotin-coupled PG4.5-DETA nanocarrier could provide an effective, targeted drug delivery system and selectively convey gemcitabine into tumor cells.

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Glycine-modified polyamidoamine dendrimers: synthesis and structural characterization using nuclear magnetic resonance, ion-mobility mass spectrometry and capillary electrophoresis

Cited by 8 publications

References 30 publications

Ion Mobility Collision Cross Section Compendium

Ion Mobility Collision Cross Section Compendium

Coupling Front-End Separations, Ion Mobility Spectrometry, and Mass Spectrometry For Enhanced Multidimensional Biological and Environmental Analyses

Biotin-Decorated PAMAM G4.5 Dendrimer Nanoparticles to Enhance the Delivery, Anti-Proliferative, and Apoptotic Effects of Chemotherapeutic Drug in Cancer Cells

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