Glycine receptor distribution in mouse CNS: autoradiographic localization of [3H]strychnine binding sites

Frostholm, Adrienne; Rotter, Andrej

doi:10.1016/0361-9230(85)90038-3

Cited by 76 publications

(32 citation statements)

References 44 publications

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“…Previous reports have documented 3H-SN binding in these and other regions (Zarbin et al, 198 1;Frostholm and Rotter, 1985). Furthermore, in at least 3 nuclei, there is a striking nonuniform distribution of 'H-SN binding in the transverse plane of section.…”

Section: Discussionmentioning

confidence: 79%

“…At least in the LSO, then, the distribution of postsynaptic receptors appears to be correlated with both the quantity of postsynaptic membrane and the density of terminal arbors from the salient afferent populations. One report of 3H-SN binding con- tains an autoradiographic image of ICC that has a similar, nonuniform distribution, although there appears to be no receptor gradient in the LSO (Frostholm and Rotter, 1985).…”

Section: Anatomical Correlates To 'H-sn Bindingmentioning

confidence: 99%

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Quantitative distribution of the glycine receptor in the auditory brain stem of the gerbil

et al. 1987

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The concentration and relative distribution of glycine receptors were determined for gerbil brain stem auditory nuclei using 3H-strychnine and quantitative autoradiographic techniques. Significant binding was observed in the anteroventral cochlear nucleus, the dorsal cochlear nucleus, the lateral superior olivary nucleus, and the inferior colliculus. A non-uniform distribution of binding was seen in 3 of these nuclei, such that the greatest concentration of glycine receptors was located in the high-frequency regions. An analysis of neuron soma density suggested that the amount of post-synaptic membrane could partially explain the distribution of receptor.

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Section: Discussionmentioning

confidence: 79%

Section: Anatomical Correlates To 'H-sn Bindingmentioning

confidence: 99%

Quantitative distribution of the glycine receptor in the auditory brain stem of the gerbil

et al. 1987

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“…These bouton endings also show an affinity for >H-glycine (Schwartz, 1985). Glycine receptors can be identified on LSO principal cells using either a monoclonal antibody or jH-strychnine as a ligand (Zarbin et al, 1981;Frostholm and Rotter, 1985;Altschuler et al, 1986;Baker et al, 1986;.…”

Section: Overview Of Resultsmentioning

confidence: 99%

Synaptic pharmacology of the superior olivary complex studied in mouse brain slice

Kelly

1992

J. Neurosci.

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The synaptic pharmacology of the lateral superior olive (LSO) and medial nucleus of the trapezoid body (MNTB) was examined in a brain slice preparation of the mouse superior olivary complex (SOC) , 1967;Moore et al., 1974;Casseday and Neff, 1975;Jenkins and Masterton, 1982;Kavanagh and Kelly, 1986). Neurons in the SOC receive binaural input and are sensitive to small interaural time and intensity differences that serve as cues for sound source position (Masterton and Imig, 1984;Phillips and Brugge, 1985). In the mouse brainstem, the SOC is comprised of two main nuclei, the lateral superior olive (LSO) and the medial nucleus of the trapezoid body (MNTB). The MNTB receives its projection directly from the contralateral ventral cochlear nucleus and sends a projection to the LSO on the same side of the brain. The LSO receives a direct projection from the ipsilateral ventral cochlear nucleus. Thus, the cells in LSO are binaurally innervated through a monosynaptic ipsilateral and disynaptic contralateral pathway from the cochlear nucleus (Sto-

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“…These transmembrane proteins belong to the superfamily of pentameric Cys-loop ligand-gated channels, which also includes cation-selective nicotinic acetylcholine receptor and serotonin type3 receptor (Betz, 1990; Miller and Smart, 2010). GlyRs are predominantly expressed in the spinal cord (Young and Snyder, 1973), in the brain stem (Frostholm and Rotter, 1985; Probst et al, 1986), cerebellum (Garcia-Alcocer et al, 2008), other higher brain regions (Bristow et al, 1986), and in retina (Haverkamp et al, 2003). Functionally GlyRs participate in the movement control, perception of visual, acoustic and sensory signals and pain sensation (Harvey et al, 2004; Betz and Laube, 2006).…”

Section: Introductionmentioning

confidence: 99%

Voltage-Dependent Inhibition of Glycine Receptor Channels by Niflumic Acid

Maleeva

Peiretti

Zhorov

et al. 2017

Front. Mol. Neurosci.

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Niflumic acid (NFA) is a member of the fenamate class of nonsteroidal anti-inflammatory drugs. This compound and its derivatives are used worldwide clinically for the relief of chronic and acute pain. NFA is also a commonly used blocker of voltage-gated chloride channels. Here we present evidence that NFA is an efficient blocker of chloride-permeable glycine receptors (GlyRs) with subunit heterogeneity of action. Using the whole-cell configuration of patch-clamp recordings and molecular modeling, we analyzed the action of NFA on homomeric α1ΔIns, α2B, α3L, and heteromeric α1β and α2β GlyRs expressed in CHO cells. NFA inhibited glycine-induced currents in a voltage-dependent manner and its blocking potency in α2 and α3 GlyRs was higher than that in α1 GlyR. The Woodhull analysis suggests that NFA blocks α1 and α2 GlyRs at the fractional electrical distances of 0.16 and 0.65 from the external membrane surface, respectively. Thus, NFA binding site in α1 GlyR is closer to the external part of the membrane, while in α2 GlyR it is significantly deeper in the pore. Mutation G254A at the cytoplasmic part of the α1 GlyR pore-lining TM2 helix (level 2′) increased the NFA blocking potency, while incorporation of the β subunit did not have a significant effect. The Hill plot analysis suggests that α1 and α2 GlyRs are preferably blocked by two and one NFA molecules, respectively. Molecular modeling using Monte Carlo energy minimizations provides the structural rationale for the experimental data and proposes more than one interaction site along the pore where NFA can suppress the ion permeation.

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Glycine receptor distribution in mouse CNS: autoradiographic localization of [3H]strychnine binding sites

Cited by 76 publications

References 44 publications

Quantitative distribution of the glycine receptor in the auditory brain stem of the gerbil

Quantitative distribution of the glycine receptor in the auditory brain stem of the gerbil

Synaptic pharmacology of the superior olivary complex studied in mouse brain slice

Voltage-Dependent Inhibition of Glycine Receptor Channels by Niflumic Acid

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