Glyco-gold nanoparticle shapes enhance carbohydrate–protein interactions in mammalian cells

Sangabathuni, Sivakoti; Murthy, Raghavendra Vasudeva; Chaudhary, Preeti; Surve, Manalee Vishnu; Banerjee, Anirban; Kikkeri, Raghavendra

doi:10.1039/c6nr03008d

Cited by 34 publications

(37 citation statements)

References 44 publications

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“…Although it is well stated in the literature that in addition to a multivalent presentation, the ligand architecture [50], carbohydrate valency, density as well as carbohydrate type [51,52,53,54] and the spatial arrangement of carbohydrate ligands [55] can have an influence on carbohydrate lectin interactions, few studies have been performed on the effect of these factors on glyco-AuNP lectin binding. Kikkeri and coworkers found that the nanoparticle shape influences cellular uptake in different cancer cell lines [56] and studied the effect of heterogeneity of glycodendrons on bacterial binding [57]. Moreover, most studies showed an effect of the carbohydrate density and the spacer type and length in regard to undesirable aggregation in buffer medium [42,58,59] but only few studies investigated the effect of these factors on lectin binding [38,45].…”

Section: Introductionmentioning

confidence: 99%

Presenting Precision Glycomacromolecules on Gold Nanoparticles for Increased Lectin Binding

et al. 2017

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Glyco-functionalized gold nanoparticles have great potential as biosensors and as inhibitors due to their increased binding to carbohydrate-recognizing receptors such as the lectins. Here we apply previously developed solid phase polymer synthesis to obtain a series of precision glycomacromolecules that allows for straightforward variation of their chemical structure as well as functionalization of gold nanoparticles by ligand exchange. A novel building block is introduced allowing for the change of spacer building blocks within the macromolecular scaffold going from an ethylene glycol unit to an aliphatic spacer. Furthermore, the valency and overall length of the glycomacromolecule is varied. All glyco-functionalized gold nanoparticles show high degree of functionalization along with high stability in buffer solution. Therefore, a series of measurements applying UV-Vis spectroscopy, dynamic light scattering (DLS) and surface plasmon resonance (SPR) were performed studying the aggregation behavior of the glyco-functionalized gold nanoparticles in presence of model lectin Concanavalin A. While the multivalent presentation of glycomacromolecules on gold nanoparticles (AuNPs) showed a strong increase in binding compared to the free ligands, we also observed an influence of the chemical structure of the ligand such as its valency or hydrophobicity on the resulting lectin interactions. The straightforward variation of the chemical structure of the precision glycomacromolecule thus gives access to tailor-made glyco-gold nanoparticles (glyco-AuNPs) and fine-tuning of their lectin binding properties.

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Section: Introductionmentioning

confidence: 99%

Presenting Precision Glycomacromolecules on Gold Nanoparticles for Increased Lectin Binding

et al. 2017

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“…This was associated with variability in the relative number of mannose molecules involved in the NPs-bacteria interactions. The same group obtained similar results (higher responses of rod-modified NPs than in star and spherical particles) in in vitro experiments with mammalian cells [ 84 ].…”

Section: Glycan-functionalized Nanoparticles (Nps)mentioning

confidence: 69%

Glycan Nanobiosensors

et al. 2020

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This review paper comprehensively summarizes advances made in the design of glycan nanobiosensors using diverse forms of nanomaterials. In particular, the paper covers the application of gold nanoparticles, quantum dots, magnetic nanoparticles, carbon nanoparticles, hybrid types of nanoparticles, proteins as nanoscaffolds and various nanoscale-based approaches to designing such nanoscale probes. The article covers innovative immobilization strategies for the conjugation of glycans on nanoparticles. Summaries of the detection schemes applied, the analytes detected and the key operational characteristics of such nanobiosensors are provided in the form of tables for each particular type of nanomaterial.

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“…Label-free detection of carbohydrate-protein binding using optical scattering or surface plasmon resonance on GNPs Reynolds et al, 2012;Tsutsumi et al, 2012;Richards et al, 2016;Sangabathuni et al, 2016 Using carbohydrate-GNPs as a fluorescence quencher to investigate lectin binding Budhadev et al, 2020 Monitoring microscopic localization of GNPs in the cell through electron micrograph…”

Section: Gold Nanoparticlesmentioning

confidence: 99%

“…These particles are a very efficient light scatterer that changes their localized surface plasmon resonance frequency depending on the environment such as protein recognition of carbohydrate-coated GNPs (Tsutsumi et al, 2012 ; Richards et al, 2016 ). Therefore, GNPs have been utilized for label-free imaging and quantification of carbohydrate-protein interactions (Sangabathuni et al, 2016 ). In addition, GNPs have a strong fluorescent quenching property.…”

Section: Solid Nanoparticle Probes For Protein-carbohydrate Interactionsmentioning

confidence: 99%

Elucidating Carbohydrate-Protein Interactions Using Nanoparticle-Based Approaches

2021

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Carbohydrates are present on every living cell and coordinate important processes such as self/non-self discrimination. They are amongst the first molecular determinants to be encountered when cellular interactions are initiated. In particular, they resemble essential molecular fingerprints such as pathogen-, danger-, and self-associated molecular patterns guiding key decision-making in cellular immunology. Therefore, a deeper understanding of how cellular receptors of the immune system recognize incoming particles, based on their carbohydrate signature and how this information is translated into a biological response, will enable us to surgically manipulate them and holds promise for novel therapies. One approach to elucidate these early recognition events of carbohydrate interactions at cellular surfaces is the use of nanoparticles coated with defined carbohydrate structures. These particles are captured by carbohydrate receptors and initiate a cellular cytokine response. In the case of endocytic receptors, the capturing enables the engulfment of exogenous particles. Thereafter, the particles are sorted and degraded during their passage in the endolysosomal pathway. Overall, these processes are dependent on the nature of the endocytic carbohydrate receptors and consequently reflect upon the carbohydrate patterns on the exogenous particle surface. This interplay is still an under-studied subject. In this review, we summarize the application of nanoparticles as a promising tool to monitor complex carbohydrate-protein interactions in a cellular context and their application in areas of biomedicine.

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Glyco-gold nanoparticle shapes enhance carbohydrate–protein interactions in mammalian cells

Cited by 34 publications

References 44 publications

Presenting Precision Glycomacromolecules on Gold Nanoparticles for Increased Lectin Binding

Presenting Precision Glycomacromolecules on Gold Nanoparticles for Increased Lectin Binding

Glycan Nanobiosensors

Elucidating Carbohydrate-Protein Interactions Using Nanoparticle-Based Approaches

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