Glycoconjugate composition of ovine parotid glands elucidated by lectins

Çınar, Kenan; Öztop, Mustafa; Özkarasu, Bora

doi:10.4322/jms.085815

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…After fixation, tissues were passed through series of ascending ethanol, followed by clearing in xylene and embedding in paraffin. Paraffin sections cut at 5µm-thickness were placed onto the slides that had been pre-coated [18].…”

Section: Tissue Processingmentioning

confidence: 99%

“…Horseradish Peroxidase (HRP)-conjugated lectins were employed to explore specific sugar moieties of glycoconjugates. As described in previous study [18], the slides were dewaxed in xylene and rehydrated in descending grades of ethanol, followed by washing in distilled water. The slides were pre-incubated with 0.3% H 2 O 2 (v/v) in absolute methanol at room temperature for 10 minutes so as to eliminate endogenous peroxidase activity.…”

Section: Lectin Histochemistry (Lhc)mentioning

confidence: 99%

“…Intensity of staining was graded as negative -0, weak -1, moderate -2 and strong -3, as described in previous study [18]. [20].…”

Section: Semi-quantitative Assessmentmentioning

confidence: 99%

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Oskar Balığının (Astronotus ocellatus) Solungaçlarında Glikokonjugatların Dağılımı

Öztop

Çınar²,

Demirbağ³

2021

Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi

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Glycoconjugates involved in many functions are produced by many cell types. This study aimed to investigate the glycoconjugate expression in the Oscar fish (Astronotus ocellatus) gills using five horseradish peroxidase-conjugated lectins. This study revealed that gill epithelial cells react with Canavalia ensiformis agglutinin (Con A) and Arachis hypogaea agglutinin (PNA). Strong reaction to Con A was seen in the epithelial cells of the gill primary lamellae. Epithelial cells of primary and secondary lamellae and eosinophilic granular cells had varying intensity of PNA binding. Ulex europaeus agglutinin-I(UEA-I) positive eosinophilic granular cells were observed in the connective tissue of primary lamellae. Epithelial cells of the gill primary filament had Con A reaction in varying intensities. Superficial epithelial cells reacted with PNA in various degrees. Epithelial cells of the secondary lamellae had Con A and PNA positive reaction. These results indicate that glycoconjugate distribution may show interspecific variations, suggesting that the high heterogeneity of naturally occurring glycoconjugates in the epithelial cells of Oscar fish gills might be attributed to the various functional roles of glycoconjugates.

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Section: Tissue Processingmentioning

confidence: 99%

Section: Lectin Histochemistry (Lhc)mentioning

confidence: 99%

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Oskar Balığının (Astronotus ocellatus) Solungaçlarında Glikokonjugatların Dağılımı

Öztop

Çınar²,

Demirbağ³

2021

Süleyman Demirel Üniversitesi Fen Edebiyat Fakültesi Fen Dergisi

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Morphology of Larger Salivary Glands in Peccaries (Pecari tajacu Linnaeus, 1758)

Júnior,

Tertulino

et al. 2024

Animals

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This work aims to study the major salivary gland morphology of peccaries during their growth. The glands were analyzed using macroscopic description, light microscopy, electron microscopy, histochemistry, and immunohistochemistry. Topographically, the salivary glands resemble other animals, including domestic animals and pigs. During growth, the parotid enlarges and mandibular gland loses weight. Histologically, the parotid has serous production, and sublingual has mucous production, resembles most species, however, mandibular gland produces mucous, unlike other animals, including pigs, which produce seromucous secretion. Histochemically, parotid produces more acidic mucins than pigs and it undergoes maturation during development; mandibular, and especially the sublingual gland, produce more acidic and basic mucopolysaccharides than pigs. The results found with transmission and scanning electron microscopy techniques corroborate the histological and histochemistry findings. The major salivary glands were positive to different lecithins (Com-A, BSA-I-B4, WGA and PNA), which were also more positive than in pigs and sheep. We conclude that collared peccaries have a salivary secretion that facilitates the digestion of carbohydrates, and biometric characteristics and positivity to lecithins that facilitate adaptation to foods with antinutritional factors.

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Lectin Histochemistry of the Glycoconjugates in Partridge and Quail Conjunctival Epithelia

Öztop

Çınar

Demirbağ

2021

Van Veterinary Journal

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The aim of this study was to examine the distribution of the glycoconjugates in conjunctival epithelium of partridge (Alectoris chukar) and quail (Coturnix coturnix). Eyelid samples harvested from both species were subjected to routine tissue processing. Lectin histochemistry was applied to sections in order to demonstrate the expression of glycoconjugates. Six different HRP-conjugated lectins (Con A, UEA-I, PNA, HPA, MAA and BSA I-B4) were used for this purpose. The conjunctival epithelium in both partridge and quail consisted of goblet cells and nongoblet cells. Both PNA and HPA bound, to varying degrees, to the goblet cells and nongoblet cells in conjunctival epithelium of partridge and quail. Con A reacted with nongoblet cells in both species. While partridge conjunctival goblet cells showed no reaction to Con A, quail conjunctival goblet cells had a very weak reaction to Con A. UEA-I did not bind to any cells in partridge, however, quail conjunctival goblet cells and nongoblet cells could react to UEA-I. In both species, conjunctival epithelial surfaces reacted with Con A, UEA-I, PNA and HPA. However, MAA and BSA I-B4 did not bind any cell and/or part in both partridge and quail conjunctiva. The present data suggest that composition of glycoconjugates could be different between goblet cells and nongoblet cells of these species, but it could be very similar at conjunctival epithelial surfaces on which the content of the goblet cells and nongoblet cells is released.

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Glycoconjugate composition of ovine parotid glands elucidated by lectins

Cited by 3 publications

References 17 publications

Oskar Balığının (Astronotus ocellatus) Solungaçlarında Glikokonjugatların Dağılımı

Oskar Balığının (Astronotus ocellatus) Solungaçlarında Glikokonjugatların Dağılımı

Morphology of Larger Salivary Glands in Peccaries (Pecari tajacu Linnaeus, 1758)

Lectin Histochemistry of the Glycoconjugates in Partridge and Quail Conjunctival Epithelia

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