Glycoconjugate markers of joint diseases

Popko, Janusz; Olszewski, Sławomir; Guszczyn, Tomasz; Zwierz, Krzysztof; Pancewicz, Sławomir

doi:10.1042/bst0390331

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…Besides its role in immune system, HEXB is found stably expressed in microglia and has been used as an important marker to study central nervous system diseases 50 . In horses, it has been found significantly higher expressed in chondrocytes of individuals (1–4 years old) with osteochondrosis 51 due to its participation in cartilage turnover 52 . Since HEXB are associated with several diseases, further investigation on the absolute expression in adult and neonatal donkeys could help establish a basic reference for its expression and monitor for diseases in donkeys.…”

Section: Discussionmentioning

confidence: 99%

DIA mass spectrometry characterizes urinary proteomics in neonatal and adult donkeys

Feng

Chen

Liu

et al. 2022

Sci Rep

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Health monitoring is critical for newborn animals due to their vulnerability to diseases. Urine can be not only a useful and non-invasive tool (free-catch samples) to reflect the physiological status of animals but also to help monitor the progression of diseases. Proteomics involves the study of the whole complement of proteins and peptides, including structure, quantities, functions, variations and interactions. In this study, urinary proteomics of neonatal donkeys were characterized and compared to the profiles of adult donkeys to provide a reference database for healthy neonatal donkeys. The urine samples were collected from male neonatal donkeys on their sixth to tenth days of life (group N) and male adult donkeys aging 4–6 years old (group A). Library-free data-independent acquisition (direct DIA) mass spectrometry-based proteomics were applied to analyze the urinary protein profiles. Total 2179 urinary proteins were identified, and 411 proteins were differentially expressed (P < 0.05) between the two groups. 104 proteins were exclusively expressed in group N including alpha fetoprotein (AFP), peptidase-mitochondrial processing data unit (PMPCB), and upper zone of growth plate and cartilage matrix associated (UCMA), which might be used to monitor the health status of neonatal donkeys. In functional analysis, some differentially expressed proteins were identified related to immune system pathways, which might provide more insight in the immature immunity of neonatal donkeys. To the best of our knowledge, this is the first time to report donkey urinary proteome and our results might provide reference for urinary biomarker discovery used to monitor and evaluate health status of neonatal donkeys.

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Section: Discussionmentioning

confidence: 99%

DIA mass spectrometry characterizes urinary proteomics in neonatal and adult donkeys

Feng

Chen

Liu

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…ECM abnormalities can also be caused by lysosomal enzymes. In the OC cells, we detected a high abundance of beta-hexosaminidase subunit alpha and beta (HEXA -HEXB), lysosomal alpha-glucosidase, N( 4)-(beta-Nacetylglucosaminyl)-L-asparaginase and cathepsin D. These enzymes are involved in cartilage turnover [43]. Although initially PG degradation occurs extracellularly, fragments are endocytosed and Fig.…”

Section: Discussionmentioning

confidence: 99%