Glycogen Concentration in Freeze-Dried Tissues of Eastern Oyster (Crassostrea virginica) Using Near Infrared Reflectance Spectroscopy to Determine the Relationship Between Concentrations of the Tissues Excised for Histological Sampling and the Remaining Tissues

Abstract

To improve the accuracy and reproducibility of the previous near infrared reflectance spectroscopy (NIRS) model for glycogen in the oyster species Crassostrea virginica, a new model using freeze-dried samples was developed. The NIRS glycogen calibration model was developed using 380 individual oyster samples collected between 2014 and 2016 from several locations in the Chesapeake Bay. Homogenized freeze-dried samples were scanned in the near infrared region between 1,000 and 2,500 nm. In parallel, glycogen concentration (GC), measured as percent dry weight, was determined using laboratory-based methods. The two sets of data allowed us to build a NIRS model based on freeze-dried oyster meats, and the model gave a strong prediction of GC [coefficient of determination for validation (R 2 val) ¼ 0.96 and residual predictive deviation (RPD) ¼ 5.2]. The second part of the study applied the model to determine GC among 39 diploid and 40 triploid C. virginica and determined the strength of the relationship between the GC of tissues excised for histological sampling to the remaining tissue (corpus) to verify assumptions made throughout the literature. There was an estimated R 2 ¼ 0.99 between the GC in the corpus and the tissues of whole oyster meat. Among the samples, two factors, ploidy and size (shell height), had a significant effect on GC.

Comparison of the Biochemical Composition and Nutritional Quality Between Diploid and Triploid Hong Kong Oysters, Crassostrea hongkongensis

“…According to our study, in the non-reproductive phase, there were statistically significant differences in gill and gonad glycogen content between diploids and triploids, as the glycogen content of triploid Hong Kong oysters increased in response to increasing translatable mRNA of Ch GS, especially in the labial palps and gonad. The same phenomenon has been observed in C. gigas and C. virginica (Bacca et al, 2005 ; Zeng et al, 2013 ; Guévélou et al, 2017 ). Pogoda et al ( 2013 ) confirmed that O. edulis and C. gigas juveniles and adults primarily utilized glycogen to store energy, and that all tissues were capable of glycogen hydrolysis and glucose formation to provide ATP for growth.…”