Glycogen content of mouse hemidiaphragm as a biologic indicator of insulin action

Davidson, J. K.; Coddling, Judith A.; Haist, R. E.

doi:10.1139/y68-056

Cited by 9 publications

(3 citation statements)

References 8 publications

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“…8 The "unknowns" were assayed against International Insulin Standard. The standard was diluted with Gey and Gey phosphate buffer containing 10 mg. purified gelatin per ml.…”

Section: Mouse Hemidiaphragm Assays / K Davidson Department Of Physmentioning

confidence: 99%

A Consideration of Methods of Insulin Assay

Ashford¹,

Campbell²,

Davidson³

et al. 1969

Diabetes

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“…8 The "unknowns" were assayed against International Insulin Standard. The standard was diluted with Gey and Gey phosphate buffer containing 10 mg. purified gelatin per ml.…”

Section: Mouse Hemidiaphragm Assays / K Davidson Department Of Physmentioning

confidence: 99%

A Consideration of Methods of Insulin Assay

Ashford¹,

Campbell²,

Davidson³

et al. 1969

Diabetes

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“…The insulin-neutralizing capacity of patient serum was determined in 19 patients by the MHD assay method of Davidson et al 7 The amount of insulin neutralized by 1 ml. of patient serum was calculated to be the amount of insulin extractable from 1 ml.…”

Section: Methodsmentioning

confidence: 99%

Immunologic Insulin Resistance

Davidson

DeBra

1978

Diabetes

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The efficacy of sulfated beef insulin for plasma glucose control in 35 patients with immunologic insulin resistance was studied. Patients were on a mean dose of 550 U./day (range 200--2,000) of U-500 regular beef insulin. Mean maximum 125I-insulin-binding capacity was 191 mU./ml. serum (range 13--1,080). Mean in vivo half-life (T 1/2) of 125I-regular beef insulin was 614 minutes (range 114--1,300), as against a mean T 1/2 of 13.9 minutes (range 11.8--16.5) in normal controls. Treatment was successful in 34 patients and unsuccessful in one with lipoatrophic diabetes. The mean initial dose of sulfated insulin was 89 U./day (range 15--400) and at three months was 66 U./day (range 20--400). Twenty-eight patients who responded and survived have been on sulfated insulin for a mean of 39 months (range 2-66) and are on a mean dose of 25 U./day (range 0--100). The mean maximum binding capacity fell to 9 mU./ml. (range 0--34) during therapy (p less than 0.01). Mean 125I-insulin T 1/2 fell from 614 to 249 minutes after sulfated insulin therapy (p less than 0.001). A comparative study of 15 patients on consecutive days showed a 35 sulfated insulin T 1/2 of 60 minutes (range 15--94) and a mean 125I-regular insulin T 1/2 of 246 minutes (range 62--560, p less than 0.001). These results indicate that sulfated insulin is less antigenic than regular beef insulin and combines less avidly with human antibodies to regular beef insulin. The response to sulfated insulin therapy was significantly better than the response reported by other investigators to pork insulin or to steroid therapy in similar patients.

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“…2 The pancreatic extract was assayed for its insulin activity content by the mouse hemidiaphragm insulin assay. 3 It contained 0.50 U. insulin per gram. Preparation of guinea pig sera containing antibodies to beef insulin, to chicken insulin, and to cod insulin Guinea pig antibodies to beef insulin (Ab x ), to chicken insulin (Ab 2 ), and to cod insulin (Ab 3 ) were prepared by the method of Moloney and Coval 4 and their neutralizing potency determined as previously 3 described.…”

Section: Preparation and Assay Of Capybara Pancreatic Insulin Activitymentioning

confidence: 99%

Failure of Guinea Pig Antibodies to Beef Insulin, Chicken Insulin, and Cod Insulin to Neutralize Capybara Insulin

1969

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Guinea pig and coypu insulins are the only mammalian insulins known to be non-neutralizable by antibody to beef insulin. A third non-neutralizable mammalian insulin, that of the capybara (carpincho, or hydrochoerus hydrochoerus) has been identified. Capybara pancreas yielded 0.5 U. extractable insulin per gram. An amount of guinea pig antibody to beef insulin sufficient to neutralize thirty times as much beef insulin did not significantly alter the increase in glycogen content produced by pancreatic capybara insulin in the mouse hemidiaphragm in vitro. Both 50 mU. capybara insulin and 50 mil capybara insulin plus 150 mU. antibody to beef insulin when injected into mice produced about the same number of convulsions and lowered the blood glucose to about the same mean level as did 50 raU. beef insulin alone. Mice injected with 50 mU. beef insulin plus 150 mU. antibody to beef insulin did not convulse and had a mean blood glucose of 156 mg. per 100 ml. Antibody to chicken insulin and antibody to cod insulin did not significantly alter the increase in glycogen content produced by pancreatic capybara insulin in the mouse hemidiaphragm in vitro. Capybara pancreatic extract cross-reacted with guinea pig antiserum to beef insulin in the passive cutaneous ana-phylaxis test.

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Glycogen content of mouse hemidiaphragm as a biologic indicator of insulin action

Cited by 9 publications

References 8 publications

A Consideration of Methods of Insulin Assay

A Consideration of Methods of Insulin Assay

Immunologic Insulin Resistance

Failure of Guinea Pig Antibodies to Beef Insulin, Chicken Insulin, and Cod Insulin to Neutralize Capybara Insulin

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