Glycogen depletion in the newborn rat liver an electron microscopic and electron histochemical study

Phillips, M. J.; Unakar, Nalin J.; Doornewaard, Gerald; Steiner, Jan W.

doi:10.1016/s0022-5320(67)80236-3

Cited by 77 publications

(27 citation statements)

References 56 publications

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“…This is supported by similar obser vations in other species [22,[24][25][26] and by our previous finding that in the dog the in vitro activities of several microsomal en zymes increase in postnatal life [33]. The increase in SER could also be related to that in bile secretory activity, since stimulation of microsomal function is known to be associ ated with an increase in bile secretion [34,35].…”

Section: Discussionsupporting

confidence: 77%

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Bile Secretory Apparatus in the Newborn Dog: Relationship between Structural and Functional Immaturities

Tavoloni

Schaffner²

1989

Neonatology

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In the dog, bile secretion is not fully mature at birth and develops during postnatal life. To try to establish morphologic correlates to the physiologic deficiencies, we examined the ultrastructure of hepatic parenchyma and biliary epithelium in a newborn puppy and in 3 puppies of 1, 3, and 7 days of age. At birth, the hepatocytes contain much glycogen and fat droplets, a small smooth endoplasmic reticulum and Golgi apparatus, rare autophagic vacuoles, and numerous lysosomes. The sinusoidal microvilli are short, and sub-membrane vesicles are few and small. The bile canaliculus is not dilated, but few and short microvilli and no pericanalicular vesicles are seen. The biliary epithelial cells are normal in size, but the luminal surface of the bile ductule has no microvilli and numerous blebs. These morphologic features change with maturation and, by the first week of life, the fine structure of the hepatocytes, bile ductular cells, and biliary passages resemble that observed in the adult liver. These findings provide morphologic support for the concept that, in the dog, the bile secretory apparatus is immature at birth and develops during postnatal life.

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Section: Discussionsupporting

confidence: 77%

“…The finding that the newborn dog liver contains large amounts of glycogen is not surprising since it has been documented in a variety of species [22][23][24][25][26]. While the mecha nism of perinatal glycogen accumulation is not known, the significance of this event is becoming increasingly clear.…”

Section: Discussionmentioning

confidence: 99%

Bile Secretory Apparatus in the Newborn Dog: Relationship between Structural and Functional Immaturities

Tavoloni

Schaffner²

1989

Neonatology

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“…Glycogen is also an important energy source for tissues during the neonatal period. Glycogen granules are found abundantly within autophagosomes of various neonatal tissues (Phillips et al 1967, Schiaffino & Hanzlikova 1972, and glucose produced by autophagy can be utilized in the working muscles of neonates (Schiaffino et al 2008). OVX uteri deprived of steroid hormones for weeks have low glycogen content compared with cycling or hormone-treated mice, whereas E 2 -injected uteri accumulate glycogen at levels several times higher than OVX uteri (Ahmed-Sorour & Bailey 1981).…”

Section: Autophagic Response In Isolated Mouse Uterine Cellsmentioning

confidence: 99%

Suppression of autophagic activation in the mouse uterus by estrogen and progesterone

Choi¹,

Shin²,

Song

et al. 2014

Journal of Endocrinology

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Autophagy is a major cellular catabolic pathway tightly associated with cell survival. The involvement of autophagy in the prolonged survival of blastocysts in the uterus is well established, and it was assumed that ovarian steroid hormones -progesterone (P 4 ) and estrogens -have important roles in the regulation of autophagy. However, information is scarce regarding whether these hormones regulate autophagy in certain hormoneresponsive cellular systems. In this study, we investigated the effects of estrogen and P 4 on autophagic response in the uteri of pregnant mice and in ovariectomized (OVX) mice treated with hormones. During pregnancy, autophagic response is high on days 1 and 2 when the uterus shows an inflammatory response to mating, but it subsides around the time of implantation. Dexamethasone treatment to day 1 pregnant mice reduced autophagy in the uterus. In OVX mouse uteri, estrogen or P 4 reduces autophagic response within 6 h. Glycogen content in OVX uteri was increased by 3-methyladenine treatment, suggesting that autophagy is involved in glycogen breakdown in the hormone-deprived uterus. The classical nuclear receptor antagonists, ICI 182 780 or mifepristone, lead to the recovery of the autophagic response in OVX uteri. The suppression of autophagy by 17b-estradiol is inversely correlated with the accumulation of phospho-mouse target of rapamycin, and rapamycin treatment is moderately effective in the upregulation of autophagic response in OVX mouse uteri. Collectively, this study establishes that the uterine autophagy is induced in hormone-derived environment and is suppressed by hormone treatment. Uterine autophagy may have multiple functions as a responsive mechanism to acute inflammation and as an energy provider by breaking down glycogen under hormone deprivation.

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“…In th e 14-day-old ra ts, glvcogen was occasionally seen enclosed in m em brane-hound vesicles ( fig.4) b u t these were sm all and not very num erous. T hey resem bled the glycogen-containing autophagic vacuoles described in th e muscles [Schiaffino and H anzlikova, 19721 and the liver [Phillips et al, 1967] of neonatal anim als. T he cells also contained num erous round or elongated m ito chondria ( fig.…”

Section: Resultsmentioning

confidence: 99%

Studies on the postnatal development of the collecting ducts of the rat kidney, with particular reference to their glycogen content

Wake¹,

Moffat²,

Creasey³

et al. 1974

Cells Tissues Organs

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Glycogen depletion in the newborn rat liver an electron microscopic and electron histochemical study

Cited by 77 publications

References 56 publications

Bile Secretory Apparatus in the Newborn Dog: Relationship between Structural and Functional Immaturities

Bile Secretory Apparatus in the Newborn Dog: Relationship between Structural and Functional Immaturities

Suppression of autophagic activation in the mouse uterus by estrogen and progesterone

Studies on the postnatal development of the collecting ducts of the rat kidney, with particular reference to their glycogen content

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