Glycogen Synthase Kinase-3 Is Required for Efficient<i>Dictyostelium</i>Chemotaxis

Teo, Regina; Lewis, Kimberley; Forde, Josephine E.; Ryves, W. J.; Reddy, Jonathan V.; Rogers, Benjamin J.; Harwood, Adrian J.

doi:10.1091/mbc.e09-10-0891

Cited by 26 publications

(57 citation statements)

References 47 publications

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“…In chapter 3, two novel PKBR1 negative regulators, the Glycogen Synthase Kinase 3 (GSK3) and the PP2A regulatory subunit, B56, will be discussed. The role of GSK3 in PKBR1 regulation has been previously reported by Teo, R. and his colleagues; cells lacking GSK3 (gsk3 -cells) exhibited undetected levels of PKBR1 activation and defective chemotaxis (Teo, R. et al 2010; see chapter 3 below). In chapter 3, evidence suggesting GSK3 also plays a negative role in PKBR1 activation will be presented.…”

Section: The Family Of Small Gtpases Ras Regulates Leading Edge Formamentioning

confidence: 66%

“…Previous reports show conflicting evidence regarding GSK3 role in PKBR1 regulation (Teo, R. et al 2010;Chapter 3). Upon reduction in the levels of .…”

Section: Mpl1 Plays An Essential Role In Erk2 Adaptationmentioning

confidence: 99%

“…Cells lacking B56, psrA -cells, exhibited aberrantly high basal level of active PKBR1, reduced levels of PP2A activity and reduced levels of active Ras proteins; these defects resulted in higher levels of phosphorylated PKBR1 substrates and defective random and directional migration. Additionally, psrA -cells exhibited higher levels of active Glycogen synthase kinase 3 (GSK3) , which has been shown in the past to be an important PKBR1 regulator (Teo, R. et al 2010;Kölsch, V. et al 2012 FlagRasC(G13T), FlagRasD(G12T) or GSK3DN, 20µg/ml of G418 (GIBCO) was used as a selective agent for the media.…”

Section: An Important Family Of Kinases That Modulates Cytoskeletal Rmentioning

confidence: 99%

“…Recent findings show that in addition to regulating development, GSK3 plays a role in chemotaxis; gsk3 -cells exhibited defective Ras and PKBR1 regulation resulting in aberrant directional motility (Teo, R. et al 2010;Kölsch, V. et al 2012;). PKBR1 activation is impaired in gsk3 could be mediated by GSK3, we decided to reduce the levels of active GSK3 in psrA -cells by overexpressing a dominant negative form of GSK3 (GSK3DN) as previously described by Lee, N. and others (Lee, N. et al 2008); as a control, GSK3DN was also overexpressed in Wt cells.…”

Section: Gsk3 Regulates Pkbr1 Activationmentioning

confidence: 99%

“…One possible role being that GSK3 may be necessary for the transcription of cell differentiation during development . GSK3 have been shown to regulate PKBR1 activation (Teo, R. et al 2010;Kölsch, V. et al 2012). Thus, the aberrantly high levels of active PKBR1 present in psrA -cells could be due to the GSK3 hyperactivation.…”

Section: Gsk3 Regulates Pkbr1 Activationmentioning

confidence: 99%

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Two Adaptation Mechanisms Regulate Cellular Migration in Dictyostelium discouideum

Rodriguez¹

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Section: The Family Of Small Gtpases Ras Regulates Leading Edge Formamentioning

confidence: 66%

“…Previous reports show conflicting evidence regarding GSK3 role in PKBR1 regulation (Teo, R. et al 2010;Chapter 3). Upon reduction in the levels of .…”

Section: Mpl1 Plays An Essential Role In Erk2 Adaptationmentioning

confidence: 99%

Section: An Important Family Of Kinases That Modulates Cytoskeletal Rmentioning

confidence: 99%

Section: Gsk3 Regulates Pkbr1 Activationmentioning

confidence: 99%

Section: Gsk3 Regulates Pkbr1 Activationmentioning

confidence: 99%

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Two Adaptation Mechanisms Regulate Cellular Migration in Dictyostelium discouideum

Rodriguez¹

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Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes

Artemenko

Lampert

Devreotes

2014

Cell. Mol. Life Sci.

181

221

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Chemotaxis, or directed migration of cells along a chemical gradient, is a highly coordinated process that involves gradient sensing, motility, and polarity. Most of our understanding of chemotaxis comes from studies of cells undergoing amoeboid-type migration, in particular the social amoeba Dictyostelium discoideum and leukocytes. In these amoeboid cells the molecular events leading to directed migration can be conceptually divided into four interacting networks: receptor/G protein, signal transduction, cytoskeleton, and polarity. The signal transduction network occupies a central position in this scheme as it receives direct input from the receptor/G protein network, as well as feedback from the cytoskeletal and polarity networks. Multiple overlapping modules within the signal transduction network transmit the signals to the actin cytoskeleton network leading to biased pseudopod protrusion in the direction of the gradient. The overall architecture of the networks, as well as the individual signaling modules are remarkably conserved between Dictyostelium and mammalian leukocytes, and the similarities and differences between the two systems are the subject of this review.

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Aberrant spindle dynamics and cytokinesis in Dictyostelium discoideum cells that lack glycogen synthase kinase 3

Harwood

Forde-Thomas

Williams

et al. 2013

European Journal of Cell Biology

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Eukaryotic cell division requires the co-ordinated assembly and disassembly of the mitotic spindle, accurate chromosome segregation and temporal control of cytokinesis to generate two daughter cells. While the absolute details of these processes differ between organisms, there are evolutionarily conserved core components common to all eukaryotic cells, whose identification will reveal the key processes that control cell division. Glycogen synthase kinase 3 (GSK-3) is a major protein kinase found throughout the eukaryotes and regulates many processes, including cell differentiation, growth, motility and apoptosis. In animals, GSK-3 associates with mitotic spindles and its inhibition causes mis-regulation of chromosome segregation. Two suppressor screens in yeast point to a more general effect of GSK-3 on cell division, however the direct role of GSK-3 in control of mitosis has not been explored outside the animal kingdom. Here we report that the Dictyostelium discoideum GSK-3 orthologue, GskA, associates with the mitotic spindle during cell division, as seen for its mammalian counterparts. Dictyostelium possesses only a single GSK-3 gene that can be deleted to eliminate all GSK-3 activity. We found that gskA-null mutants failed to elongate their mitotic spindle and were unable to divide in shaking culture, but have no chromosome segregation defect. These results suggest further conservation for the role of GSK-3 in the regulation of spindle dynamics during mitosis, but also reveal differences in the mechanisms ensuring accurate chromosome segregation.

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Glycogen Synthase Kinase-3 Is Required for EfficientDictyosteliumChemotaxis

Cited by 26 publications

References 47 publications

Two Adaptation Mechanisms Regulate Cellular Migration in Dictyostelium discouideum

Two Adaptation Mechanisms Regulate Cellular Migration in Dictyostelium discouideum

Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes

Aberrant spindle dynamics and cytokinesis in Dictyostelium discoideum cells that lack glycogen synthase kinase 3

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