Glycogen synthase kinase 3β (GSK3β) regulates the expression of MyHC2a in goat skeletal muscle satellite cells (SMSCs)

Wang, Linjie; Zhu, Yiwen; Liu, Xin; Chao, Zhe; Wang, Yan; Zhong, Tao; Guo, Jiazhong; Zhan, Siyuan; Li, Li; Zhang, Hongping

doi:10.1111/asj.13253

Cited by 6 publications

(5 citation statements)

References 29 publications

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“…The attenuated increase of MyHC IIb mRNA in HS+A group may be caused by increased NFAT transcriptional activity, as it was shown that NFATc1 can repress MyHC IIb transcription (McCullagh et al, 2004). Partial prevention of MyHC IIa mRNA transcription decrease in HS+A group may be caused by NO-dependent GSK-3β inactivation, as it was shown that GSK-3β inhibition upregulates MyHC IIa in goat satellite cells (Wang et al, 2019).…”

Section: Discussionmentioning

confidence: 97%

NO-Dependent Mechanisms of Myosin Heavy Chain Transcription Regulation in Rat Soleus Muscle After 7-Days Hindlimb Unloading

et al. 2020

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It is known that nitric oxide (NO) may affect myosin heavy chain (MyHC) isoform mRNA transcription in skeletal muscles. The content of NO in soleus muscles decreases during rat hindlimb unloading as well as slow MyHC mRNA transcription. We aimed to detect which signaling pathways are involved in NO-dependent prevention of hindlimbsuspension (HS)-induced changes in MyHCs' expression pattern. Male Wistar rats were divided into four groups: cage control group (C), hindlimb suspended for 7 days (7HS), hindlimb suspended for 7 days with L-arginine administration (7HS+A) (500 mg/kg body mass), and hindlimb suspended for 7 days with both L-arginine (500 mg/kg) and NO-synthase inhibitor L-NAME administration (50 mg/kg) (7HS+A+N). L-arginine treatment during 7 days of rat HS prevented HS-induced NO content decrease and slow MyHC mRNA transcription decrease and attenuated fast MyHC IIb mRNA transcription increase; it also prevented NFATc1 nuclear content decrease, calsarcin-2 expression increase, and GSK-3β Ser 9 phosphorylation decrease. Moreover, L-arginine administration prevented the HS-induced myh7b and PGC1α mRNAs content decreases and slow-type genes repressor SOX6 mRNA transcription increase. All these slow fiber-type protective effects of L-arginine were blocked in HS+A+N group, indicating that these effects were NO-dependent. Thus, NO decrease prevention during HS restores calcineurin/NFATc1 and myh7b/SOX6 signaling.

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Section: Discussionmentioning

confidence: 97%

NO-Dependent Mechanisms of Myosin Heavy Chain Transcription Regulation in Rat Soleus Muscle After 7-Days Hindlimb Unloading

et al. 2020

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“…Both L-arginine administration and PMS during 7-day HS partially prevented downregulation of MyHC IIa mRNA transcription [ 66 , 114 ]. Previously it was shown that GSK-3β inhibition is able to upregulate the expression of slow myosin in chicken skeletal muscle [ 115 ] and MyHC IIa in goat satellite cells [ 116 ], however it is unclear why 3 days of PMS during unloading led to GSK-3β inhibition but did not activate MyHC IIa transcription in rat soleus muscle.…”

Section: Impact Of Gsk-3β Activity On Fiber-type Transitions and Oxidative Capacity In Skeletal Muscles Under Mechanical Unloading And Sumentioning

confidence: 99%

The Role of GSK-3β in the Regulation of Protein Turnover, Myosin Phenotype, and Oxidative Capacity in Skeletal Muscle under Disuse Conditions

Mirzoev

Sharlo

Шенкман

2021

IJMS

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Skeletal muscles, being one of the most abundant tissues in the body, are involved in many vital processes, such as locomotion, posture maintenance, respiration, glucose homeostasis, etc. Hence, the maintenance of skeletal muscle mass is crucial for overall health, prevention of various diseases, and contributes to an individual’s quality of life. Prolonged muscle inactivity/disuse (due to limb immobilization, mechanical ventilation, bedrest, spaceflight) represents one of the typical causes, leading to the loss of muscle mass and function. This disuse-induced muscle loss primarily results from repressed protein synthesis and increased proteolysis. Further, prolonged disuse results in slow-to-fast fiber-type transition, mitochondrial dysfunction and reduced oxidative capacity. Glycogen synthase kinase 3β (GSK-3β) is a key enzyme standing at the crossroads of various signaling pathways regulating a wide range of cellular processes. This review discusses various important roles of GSK-3β in the regulation of protein turnover, myosin phenotype, and oxidative capacity in skeletal muscles under disuse/unloading conditions and subsequent recovery. According to its vital functions, GSK-3β may represent a perspective therapeutic target in the treatment of muscle wasting induced by chronic disuse, aging, and a number of diseases.

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“…Studies have shown that the MSTN gene is a negative regulator of skeletal muscle growth and development and functions by controlling the number, size, and type of muscle fibers ( Bi et al, 2021 ). GSK3β regulates the expression of the MYHC-2A gene via NFATC2 to promote SMSC differentiation in goats ( Wang et al, 2019a ). The mTOR signaling pathway regulates the phosphorylation of myocyte enhancer factor 2 ( MEF2 ) through ILK , thereby affecting SMSC proliferation and differentiation ( Wu et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

A 1.1 Mb duplication CNV on chromosome 17 contributes to skeletal muscle development in Boer goats

Yuan¹,

Zhang²,

Yang³

et al. 2023

Zoological Research

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The Boer goat is one of the top meat breeds in modern animal husbandry and has attracted widespread attention for its unique growth performance. However, the genetic basis of muscle development in the Boer goat remains obscure. In this study, we identified specific structural variants in the Boer goat based on genome-wide selection signals and analyzed the basis of the molecular heredity of related candidate genes in muscle development. A total of 9 959 autosomal copy number variations (CNVs) were identified through selection signal analysis in 127 goat genomes. Specifically, we confirmed that the highest signal CNV (HSV) was a chromosomal arrangement containing an approximately 1.11 Mb (CHIR17: 60062304–61171840 bp) duplicated fragment inserted in reverse orientation and a 5 362 bp deleted region (CHIR17:60145940–60151302 bp) with overlapping genes (e.g., ARHGAP10 , NR3C2 , EDNRA , PRMT9 , and TMEM184C ). The homozygous duplicated HSV genotype (+/+) was found in 96% of Boer goats but was not detected in Eurasian goats and was only detected in 4% of indigenous African goats. The expression network of three candidate genes ( ARHGAP10 , NR3C2 , and EDNRA ) regulating dose transcription was constructed by RNA sequencing. Results indicated that these genes were involved in the proliferation and differentiation of skeletal muscle satellite cells (SMSCs) and their overexpression significantly increased the expression of SAA3 . The HSV of the Boer goat contributed to superior skeletal muscle growth via the dose effects of overlapping genes.

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Glycogen synthase kinase 3β (GSK3β) regulates the expression of MyHC2a in goat skeletal muscle satellite cells (SMSCs)

Cited by 6 publications

References 29 publications

NO-Dependent Mechanisms of Myosin Heavy Chain Transcription Regulation in Rat Soleus Muscle After 7-Days Hindlimb Unloading

NO-Dependent Mechanisms of Myosin Heavy Chain Transcription Regulation in Rat Soleus Muscle After 7-Days Hindlimb Unloading

The Role of GSK-3β in the Regulation of Protein Turnover, Myosin Phenotype, and Oxidative Capacity in Skeletal Muscle under Disuse Conditions

A 1.1 Mb duplication CNV on chromosome 17 contributes to skeletal muscle development in Boer goats

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