2008
DOI: 10.1074/jbc.m801756200
|View full text |Cite
|
Sign up to set email alerts
|

Glycogen Synthase Kinase 3β Is a Novel Regulator of High Glucose- and High Insulin-induced Extracellular Matrix Protein Synthesis in Renal Proximal Tubular Epithelial Cells

Abstract: High glucose (30 mM) and high insulin (1 nM), pathogenic factors of type 2 diabetes, increased mRNA expression and synthesis of laminin ␤1 and fibronectin after 24 h of incubation in kidney proximal tubular epithelial (MCT) cells. We tested the hypothesis that inactivation of glycogen synthase kinase 3␤ (GSK3␤) by high glucose and high insulin induces increase in synthesis of laminin ␤1 via activation of eIF2B⑀. Both high glucose and high insulin induced Ser-9 phosphorylation and inactivation of GSK3␤ at 2 h t… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

5
66
2

Year Published

2012
2012
2022
2022

Publication Types

Select...
6
2
1

Relationship

4
5

Authors

Journals

citations
Cited by 64 publications
(73 citation statements)
references
References 60 publications
5
66
2
Order By: Relevance
“…Previous studies have revealed that using small interfering (si)RNA against GSK-3β can cause transdifferentiation of mammary gland tissue and skin epithelial cells (59). In addition, the activity of GSK-3β may maintain the structure of epithelial cells and their surface role in the normal podocyte phenotype (60,61). In contrast with these data, the present study revealed that normal kidney tissue expressed low levels of β-catenin and Snail.…”
Section: Discussioncontrasting
confidence: 55%
“…Previous studies have revealed that using small interfering (si)RNA against GSK-3β can cause transdifferentiation of mammary gland tissue and skin epithelial cells (59). In addition, the activity of GSK-3β may maintain the structure of epithelial cells and their surface role in the normal podocyte phenotype (60,61). In contrast with these data, the present study revealed that normal kidney tissue expressed low levels of β-catenin and Snail.…”
Section: Discussioncontrasting
confidence: 55%
“…Immunoblotting-Immunoblotting was performed as described previously (13)(14)(15). Equal amounts of cell lysate protein (2-20 g) and tissue homogenates (30 g) were separated by SDS-PAGE and transferred to a nitrocellulose membrane.…”
Section: Methodsmentioning
confidence: 99%
“…Protein Detection-Immunoblotting was performed as described (11,12). All primary antibodies were from Cell Signaling Technology (Danvers, MA) except for those against fibronectin, CSE, laminin ␥1 (Santa Cruz Biotechnology), and cystathionine ␤-synthase (Abgent).…”
Section: Methodsmentioning
confidence: 99%
“…In addition, recent work has shown that high glucose suppresses kinases that normally inhibit protein synthesis, e.g. AMP-activated protein kinase (AMPK) (7)(8)(9)(10) and glycogen synthase kinase 3␤ (11). These observations have suggested that the control of pathologically increased protein synthesis could be achieved by the activation of inhibitory kinases.…”
mentioning
confidence: 99%