Glycogen Synthase Kinase Is a Regulator of the Circadian Clock of Neurospora crassa

Tataroglu, Ozgur; Lauinger, Linda; Sancar, Gencer; Jakob, Katharina; Brunner, Michael; Diernfellner, Axel

doi:10.1074/jbc.m112.396622

Cited by 23 publications

(21 citation statements)

References 42 publications

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“…We next characterized phosphorylation of FRQ by recombinant CK1a in a cellfree system 26 . Light-grown Neurospora expresses high levels of heterogeneously phosphorylated FRQ.…”

Section: Resultsmentioning

confidence: 99%

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The RNA helicase FRH is an ATP-dependent regulator of CK1a in the circadian clock of Neurospora crassa

et al. 2014

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The Neurospora clock protein FRQ forms a complex with casein kinase 1a (CK1a) and FRH, a DEAD box-containing RNA helicase with a clock-independent essential function in RNA metabolism. In the course of a circadian period, FRQ is progressively hyperphosphorylated and eventually degraded. Timed hyperphosphorylation of FRQ is crucial for timekeeping of the clock. Here we show that the ATPase activity of FRH attenuates the kinetics of CK1a-mediated hyperphosphorylation of FRQ. Hyperphosphorylation of FRQ is strictly dependent on site-specific recruitment of a CK1a molecule that is activated upon binding. The FRH ATPase cycle regulates the access of CK1a to phosphorylation sites in FRQ in cis, suggesting that FRH is an ATP-dependent remodelling factor acting on the protein complex. We show that the affinity of CK1a for FRQ decreases with increasing FRQ phosphorylation, suggesting functional inactivation of FRQ in the negative feedback loop of the circadian clock before and independent of its degradation.

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“…We next characterized phosphorylation of FRQ by recombinant CK1a in a cellfree system 26 . Light-grown Neurospora expresses high levels of heterogeneously phosphorylated FRQ.…”

Section: Resultsmentioning

confidence: 99%

“…The samples were immediately re-buffered into Neurospora protein extraction buffer (without glycerol) using PD-10 columns (GE Healthcare). Glycerol was added after re-buffering 26 ). FRH (residues 1-1,106) and FRHDN (residues 116-1,106) were expressed with an N-terminal His 6 -GST-tag followed by a TEV cleavage site in E. coli BL21(DE3) cells.…”

Section: Methodsmentioning

confidence: 99%

The RNA helicase FRH is an ATP-dependent regulator of CK1a in the circadian clock of Neurospora crassa

et al. 2014

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“…CK-1a and CK-II phosphorylate and inactivate WCC in a FRQ-dependent (piggy-back) manner (He et al , 2006), whereas PP2A and PP4 dephosphorylate and activate WCC (Schafmeier et al , 2005; Cha et al , 2007). Another kinase, GSK3, regulates WCC abundance by promoting its degradation (Tataroğlu et al , 2012). It is difficult to elucidate the specific roles these kinases and phosphatases play in the N. crassa light response given that they have multiple targets and the final molecular phenotype might be a combination of several light response-independent molecular changes.…”

Section: N Crassa: a Model Organism For Studying Photobiologymentioning

confidence: 99%

Seeing the world differently: variability in the photosensory mechanisms of two model fungi

Dasgupta

Fuller

Dunlap

et al. 2015

Environmental Microbiology

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Summary Light plays an important role for most organisms on this planet, serving either as a source of energy or information for the adaptation of biological processes to specific times of day. The fungal kingdom is estimated to contain well over a million species, possibly ten-fold more, and it is estimated that a majority of the fungi respond to light, eliciting changes in several physiological characteristics including pathogenesis, development and secondary metabolism. Two model organisms for photobiological studies have taken center-stage over the last few decades – Neurospora crassa and Aspergillus nidulans. In this review, we will first discuss our understanding of the light response in N. crassa, about which the most is known, and will then juxtapose N. crassa with A. nidulans which, as will be described below, provides an excellent template for understanding photosensory cross-talk. Finally, we will end with a commentary on the variability of the light response among other relevant fungi, and how our molecular understanding in the aforementioned model organisms still provides a strong base for dissecting light responses in such species.

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“…The N. crassa glycogen synthase kinase GSK-3 (encoded by NCU04185) impacts the circadian clock by regulation of the abundance of the photoreceptor complex WCC on a posttranscriptional level. Thereby, GSK-3 binds and phosphorylates WC-1 and WC-2, hence influencing accumulation of the complex (741).…”

Section: Protein Kinasesmentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

166

195

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SUMMARYThe genusTrichodermacontains fungi with high relevance for humans, with applications in enzyme production for plant cell wall degradation and use in biocontrol. Here, we provide a broad, comprehensive overview of the genomic content of these species for “hot topic” research aspects, including CAZymes, transport, transcription factors, and development, along with a detailed analysis and annotation of less-studied topics, such as signal transduction, genome integrity, chromatin, photobiology, or lipid, sulfur, and nitrogen metabolism inT. reesei,T. atroviride, andT. virens, and we open up new perspectives to those topics discussed previously. In total, we covered more than 2,000 of the predicted 9,000 to 11,000 genes of eachTrichodermaspecies discussed, which is >20% of the respective gene content. Additionally, we considered available transcriptome data for the annotated genes. Highlights of our analyses include overall carbohydrate cleavage preferences due to the different genomic contents and regulation of the respective genes. We found light regulation of many sulfur metabolic genes. Additionally, a new Golgi 1,2-mannosidase likely involved inN-linked glycosylation was detected, as were indications for the ability ofTrichodermaspp. to generate hybrid galactose-containingN-linked glycans. The genomic inventory of effector proteins revealed numerous compounds unique toTrichoderma, and these warrant further investigation. We found interesting expansions in theTrichodermagenus in several signaling pathways, such as G-protein-coupled receptors, RAS GTPases, and casein kinases. A particularly interesting feature absolutely unique toT. atrovirideis the duplication of the alternative sulfur amino acid synthesis pathway.

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Glycogen Synthase Kinase Is a Regulator of the Circadian Clock of Neurospora crassa

Cited by 23 publications

References 42 publications

The RNA helicase FRH is an ATP-dependent regulator of CK1a in the circadian clock of Neurospora crassa

The RNA helicase FRH is an ATP-dependent regulator of CK1a in the circadian clock of Neurospora crassa

Seeing the world differently: variability in the photosensory mechanisms of two model fungi

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

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