Glycogenolytic action of glucagon-family peptides and epinephrine on catfish hepatocytes

Ottolenghi, C; Puviani, A.C.; Gavioli, Marthe; Fabbri, Elena; Brighenti, L; Plisetskaya, Erika M.

doi:10.1007/bf01875608

Cited by 19 publications

(11 citation statements)

References 34 publications

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“…As expected (Brighenti et al 1987;Ottolenghi et al 1988Ottolenghi et al , 1989, these two hormones elevated CAMP levels and phosphorylase a activity in both pools of liver cells. They also decreased the glycogen content in hepatocytes and stimulated glucose release into the incubation medium.…”

Section: Discussionsupporting

confidence: 61%

Separation of two populations of fish hepatocytes by digitonin infusion: some metabolic patterns and hormonal responsiveness

Ottolenghi¹,

Ricci²,

Gavioli³

et al. 1991

Can. J. Zool.

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1991. Separation of two populations of fish hepatocytes by digitonin infusion: some metabolic patterns and hormonal responsiveness. Can. J. Zool .69: 427-435. Two pools of liver cells, enriched either in periportal or in perivenous hepatocytes, were obtained from the catfish Ictalurus melas by the selective destruction of the liver by digitonin infusion. Following digitonin pulses directed either orthograde via the portal vein or retrograde via the hepatic vein, hepatocytes from undamaged perivenous and periportal zones, respectively, were isolated by the conventional collagenase perfusion procedure. Eluates from either periportal or perivenous zones were collected for a total of 9 min before, during, and immediately following the digitonin pulse. The eluates were assayed for protein, glycogen, glucose, and activities of lactate dehydrogenase and alanine aminotransferase, and their yield ("recovery") was estimated as a percentage of the total hepatic content. The aforementioned metabolic compounds and enzyme activities were measured also in periportalend perivenous liver cells. No significant differences were obtained between these cells. Both pools of hepatocytes were more than 90% viable and remained hormone responsive. Epinephrine (3 X lop8 M) or salmon glucagon (3 x M) elevated CAMP content, and stimulated glycogen phosphorylase a activity, glycogen depletion , and glucose release in periportal and perivenous liver cells. No differences were observed between periportal and perivenous cells in their metabolic responsiveness to either hormone. OTTOLENGHI, C., RICCI, D., GAVIOLI, M. E., PUVIANI, A. C., FABBRI, E., CAPUZZO, A., BRIGHENTI, L., et PLISETSKAYA, E. M. 1991. Separation of two populations of fish hepatocytes by digitonin infusion: some metabolic patterns and hormonal responsiveness.Can. J. Zool. 69 : 427-435. Deux populations de cellules hkpatiques, enrichies d'hkpatocytes pkriportaux ou pkriveineux, ont kt6 obtenues chez Ictalurus melas par destruction selective du foie par infusion de digitonine. Aprks une courte infusion de digitonine en direction orthograde, via la veine portale, ou rktrograde, via la veine hkpatique, les hkpatocytes intacts des zones Nriveineuse et @riportale ont kt6 isolks par un traitement classique a la collagknase. Des klutriats de la zone @riportale ou de la zone griveineuse ont kt6 recueillis durant 9 min avant, pendant ou immkdiatement aprks le traitement a la digitonine. Les fractions protkine, glycogkne et glucose de mCme que I'activitk des enzymes lactate dCshydrogknase et alanine-aminotransfkrase, ont kt6 analyskes et leur production (fraction rkcupkrke), estimke en pourcentage de leur concentration totale dans le foie. Les produits de mktabolisme et activitks enzymatiques mentionnks ci-haut ont kgalement kt6 mesurks dans les cellules hkpatiques firiportales et pkriveineuses. I1 n'y avait pas de diffkrences significatives entre ces cellules. Les deux populations d'hkpatocytes se sont avkrkes viables a 90% et sont restkes sensibles a I'action des hormones. De l'kpinkphri...

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Section: Discussionsupporting

confidence: 61%

Separation of two populations of fish hepatocytes by digitonin infusion: some metabolic patterns and hormonal responsiveness

Ottolenghi¹,

Ricci²,

Gavioli³

et al. 1991

Can. J. Zool.

Self Cite

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“…Our initial report on the absence of GLP-dependent cAMP increases in rainbow trout (Oncorhynchus mykiss) hepatocytes (Mommsen et al 1987), although confirmed for this species, had to be revised for other teleosts . Nevertheless, even in species where GLP-independent increases in cAMP were detected, a clear correlation between cAMP levels and biochemical output of the targetted cells was found wanting (Ottolenghi et al 1989;Mommsen and Moon 1990). Statistically significant increases in glycogenolytic or gluconeogenic rates, for instance, are observed at high picomolar concentrations of pGLPs, while increases in cAMP are not noted until hormone level exceed the low nanomolar level (Ottolenghi et al 1989;Mommsen and Moon 1990).…”

Section: Fishesmentioning

confidence: 86%

“…Nevertheless, even in species where GLP-independent increases in cAMP were detected, a clear correlation between cAMP levels and biochemical output of the targetted cells was found wanting (Ottolenghi et al 1989;Mommsen and Moon 1990). Statistically significant increases in glycogenolytic or gluconeogenic rates, for instance, are observed at high picomolar concentrations of pGLPs, while increases in cAMP are not noted until hormone level exceed the low nanomolar level (Ottolenghi et al 1989;Mommsen and Moon 1990). This discrepancy may even be exacerbated by the increased sensitivity of isolated fish cells maintained in perifusion.…”

Section: Fishesmentioning

confidence: 86%

Metabolic and endocrine functions of glucagon-like peptides — evolutionary and biochemical perspectives

Mommsen

Plisetskaya

1993

Fish Physiol Biochem

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In amphibians and mammals, the preproglucagon gene encodes two related GLPs - GLP-1(37 residues) and GLP-2 (34 residues). The physiologically functional GLPs, however, are GLP-17-36amide, or similar, truncated forms of GLP-1. While the liver is devoid of GLP receptors and fails to respond metabolically, brain, gastric cells and pancreatic cells have been identified as potential target sites. These tissues possess specific binding sites for truncated GLP-1, and generally, cells respond to truncated GLPs with increases in CAMP. In mammalian pancreas, truncated GLPs function as powerful insulinotropins, while also increasing insulin gene transcription and inhibiting glucagon release. Full length GLP-1 is a weak insulinotropin only. To date, neither metabolic nor endocrine actions have been assigned to GLP-2.Following processing of preproglucagon gene products, fish pancreas and intestine contain only one GLP (31 residues) displaying considerable sequence homology with GLP-17-36. Applied in low nanomolar concentrations, fish GLPs, together with GLP-17-36, activate hepatic glycogenolysis, gluconeogenesis and lipolysis in fishes. Thousand-fold higher levels of GLP-17-37 are required to elicit comparable metabolic effects. Generally, the metabolic actions of GLPs are similar to those for glucagon, but GLPs tend to be more potent in their specific actions. Fish livers possibly contain GLP receptors distinct from glucagon receptors. Message transduction in some, but not all fish species, may involve adenylyl cyclase and CAMP, but correlation between metabolic activation and cAMP levels is generally poor. Fish GLP seems to be a very weak insulinotropin in Brockmann bodies.

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“…The glycogenolytic action of catecholamines during stress is well established (Janssens and Waterman, 1988;Ottolenghi et al, 1989;Fabri et al, 1998) but there is also evidence that cortisol makes an important contribution to glycogen mobilization (Paxton et. al., 1984;Morales et al, 1990;Soengas et al, 1992bSoengas et al, , 1993Reubush and Heath, 1996).…”

Section: Discussionmentioning

confidence: 99%

Divergence of endocrine and metabolic responses to stress in two rainbow trout lines selected for differing cortisol responsiveness to stress

Trenzado

Carrick

Pottinger

2003

General and Comparative Endocrinology

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Abstract. Rainbow trout (Oncorhynchus mykiss) of two lines selected for low (LR) and high (HR) cortisol stress-responsiveness were subjected to confinement for a period of 336 hours.Endocrine (plasma cortisol, hepatic cortisol binding) and metabolic (plasma glucose, lactate, amino acids; hepatic glycogen and alanine aminotransferase levels) indices of stress were measured at intervals in confined and unconfined fish of both lines. During confinement plasma cortisol concentration reached maximum values earlier in HR fish (2 hours) than in LR fish (6 hours) returning to control values within 336 hours in both lines. Paradoxically, although both HR and LR lines displayed a characteristic metabolic stress response, these changes were more pronounced in LR fish. Plasma glucose and lactate levels increased during confinement in both lines but to a significantly greater extent in LR fish. Confinement significantly elevated plasma amino acids to a greater extent in LR fish than in HR fish. Liver glycogen concentration was depleted most rapidly in LR fish but was significantly higher in confined fish of both lines than controls at the end of the experiment. No significant changes were observed in hepatic alanine aminotransferase activity during confinement. Confined fish of both lines displayed a decrease in hepatic cortisol receptor abundance within 24 h and this was more sustained in HR fish. The more pronounced disturbance of a broad range of indicators of stress in confined LR fish, compared to HR fish, throws doubt on the magnitude of the cortisol response being the primary driver of these differences.

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Glycogenolytic action of glucagon-family peptides and epinephrine on catfish hepatocytes

Cited by 19 publications

References 34 publications

Separation of two populations of fish hepatocytes by digitonin infusion: some metabolic patterns and hormonal responsiveness

Separation of two populations of fish hepatocytes by digitonin infusion: some metabolic patterns and hormonal responsiveness

Metabolic and endocrine functions of glucagon-like peptides — evolutionary and biochemical perspectives

Divergence of endocrine and metabolic responses to stress in two rainbow trout lines selected for differing cortisol responsiveness to stress

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