Glycohistochemical study of the toadfish <em>Halobatrachus didactylus</em> (Scheider, 1801) stomach

Abstract

SuMMarY: toadfish Halobatrachus didactylus gastric mucosa was studied using conventional and lectin histochemistry. conventional histochemistry revealed neutral glycoconjugates predominating over acidic ones in the apical zone of both surface epithelial cells and pit cells. the neck cells contained a few neutral glycoconjugates, whereas gastric glands were negative to PaS and aB staining. lectin histochemistry showed different oligosaccharide expression along the columnar cells. the sub-nuclear cytoplasm was stained with rca 120 , SBa, hPa, GSa i-B 4 , GSa ii, uea i, lta, con a, Kohsialidase-WGa. the Golgi zone reacted with rca 120 , DBa, SBa, hPa, Koh-sialidase-WGa, GSa i-B 4 , GSa ii, uea i, lta, Mal ii, Sna, and showed an increase in DBa staining after Koh-sialidase treatment. the granules of the apical zone stained with Pna, uea i, lta and showed increased Pna reactivity after Koh-sialidase treatment. the luminal cell coat reacted with Pna, hPa, con a, Koh-sialidase-WGa, uea i, lta, Mal ii, Sna and Koh-sialidase-Pna. Pit cells showed a minor expression of lectin-binding sites with respect to columnar cells. neck cells linked uea i and lta and gastric glands reacted with Pna, DBa, SBa, hPa, con a, GSa i-B 4 , Koh-sialidase-WGa and Koh-sialidase-DBa. the results suggest that the stomach of the toadfish H. didactylus is characterised by a species-specific glycoconjugate pattern.Keywords: glycoconjugates, lectin histochemistry, stomach, toadfish.reSuMen: Expresión glucohistoquímica en el estómago del pez sapo, HalobatracHus didactylus (Schneider, 1801). -Se ha estudiado la expresión de residuos glucídicos de glucoconjugados en la mucosa gástrica del pez sapo, Halobatrachus didactylus usando histoquímica convencional y de lectinas. la histoquímica clásica reveló la presencia de glicoconjugados neutros predominando sobre los ácidos en la zona apical de la superficie de las células epiteliales y de la criptas gástricas. las células del cuello contienen algunas glicoproteínas neutras, mientras las glándulas gástricas han sido negativas al PaS y al azul alcián (aB). la histoquímica de lectinas mostró diferente expresión de oligosacáridos a lo largo de las células columnares. el citoplasma sub-nuclear reaccionó con las lectinas rca, SBa, hPa, GSa, i-B4, GSa ii, uea i, lta, con a, Koh-sialidasa-WGa. la expresión de rca 120 , DBa, SBa, hPa, Koh-sialidasa-WGa, GSa i-B 4 , GSa ii, uea i, lta, Mal ii y Sna se localizó en la zona del Golgi, donde se observó un incremento de la reactividad de la lectina DBa después del tratamiento con Koh. los gránulos de la zona apical reaccionaron con Pna, uea i, lta, mostrando un incremento de la reactividad hacia la lectina Pna después del tratamiento con Koh. el borde de las células del lumen reaccionó con Pna, hPa, cona, Koh-sialidasa-WGa, uea i, lta, Mal ii, Sna, Koh-sialidasa-Pna. los sitios de unión de la expresión de las lectinas-glucoconjugados fue menor en las células del cuello de las criptas gastricas que en las células columnares epiteliales. las células del cuello reaccionaro...

Goblet Cell Types in Intestine of Tiger Barb and Black Tetra (Cyprinidae, Characidae: Teleostei)

“…The composition of mucus in the digestive tract of teleosts appears specific for each part of the tract and varies significantly from species to species, suggesting various roles such as lubrication and mechanical protection, defence against parasites and pathogenic bacteria, protection against the gastric juices, ionic and osmotic regulation, digestion and absorption (Tibbetts, ; Baglole et al., ; Roberton and Wright, ; Arellano et al., , ; Morrison and Wright, ; Sarasquete et al., ; Pedini et al., ; Díaz et al., , , ,b; Domeneghini et al., , ; Carrassón et al., ; Cinar and Senol, ; Jaroszewska et al., ; Desantis et al., ; Xiong et al., ). Thus, in the common carp ( Cyprinus carpio ) intestine, some types of bacterial infection may destroy the mucin, whereas others may stimulate the mucus cells to change its glycoprotein types or to increase the production rate of mucin (Schroers et al., ).…”

Mucin in epithelial cells in oesophagus and stomach of black tetra, Gymnocorymbus ternetzi (Characidae, Teleostei)

“…However, in some species from the order Perciformes, the mucin in the stomach surface epithelial layer seems to be mainly neutral (Hernandez et al 2001;Carrassón et al 2006). Also, in toadfish, (Halobatrachus didactylus), order Batrachoidiformes, the mucin in the stomach surface cells consists predominately of neutral glycoproteins (Desantis et al 2009). In addition, an entirely neutral stomach mucin has recently been reported in a species from the characid genus Hyphessobrycon (Leknes 2011).…”

Mucus Cells in the Digestive Tract in Prenatal Platyfish Larvae (Poeciliidae, Teleostei)

“…The composition of mucus in the digestive tract of teleosts appears specific for each part of the tract and varies significantly from species to species, suggesting various roles of this substance, e.g. lubrication and mechanical protection, defence against parasites and pathogenic bacteria, ionic and osmotic regulation, digestion and absorption (Baglole et al., 1997; Tibbetts, 1997; Domeneghini et al., 1998, 2005; Arellano et al., 1999, 2001; Morrison and Wright, 1999; Pedini et al., 2001; Sarasquete et al., 2001; Diaz et al., 2003, 2006, 2008a,b; Carrasson et al., 2006; Cinar and Senol, 2006; Desantis et al., 2009).…”