Glycolate oxidase-dependent H2O2 production regulates IAA biosynthesis in rice

Li, Xiangyang; Liao, Mengmeng; Huang, Jiayu; Xu, Zhongyu; Lin, Zhanqiao; Ye, Nenghui; Zhang, Zhisheng; Peng, Xinxiang

doi:10.1186/s12870-021-03112-4

Cited by 21 publications

(20 citation statements)

References 62 publications

(92 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This conclusion is also veri ed by the fact that the trends of methionine, threonine, and isoleucine are the same regardless of whether GB or cycloleucine is sprayed. As for tryptophan, a synthetic precursor of IAA 70 , the trends of tryptophan and IAA contents in the plants were consistent after GB application. However, the IAA and tryptophan contents of plants sprayed with cycloleucine decreased.…”

Section: Discussionmentioning

confidence: 66%

Effects of Exogenous Glycine Betaine and Cycloleucine on Photosynthetic Capacity, Amino Acid Composition, and Hormone Metabolism in Solanum melongena L

Niu

Zhang

et al. 2022

Preprint

View full text Add to dashboard Cite

Although exogenous glycine betaine and cycloleucine have been reported to affect animal cell metabolism, their effects on plant growth and development have not been studied extensively. Different concentrations of exogenous glycine betaine (20, 40, and 60 mmol L− 1) and cycloleucine (10, 20, and 40 mmol L− 1), with 0 mmol L− 1 as control, were used to investigate the effects of foliar spraying of betaine and cycloleucine on growth, photosynthesis, chlorophyll fluorescence, Calvin cycle pathway, abaxial leaf burr morphology, endogenous hormones, and amino acid content in eggplant. We found that 40 mmol L− 1 glycine betaine had the best effect on plant growth and development; it increased the fresh and dry weight of plants, increased the density of abaxial leaf hairs, increased the net photosynthetic rate and Calvin cycle key enzyme activity of leaves, had an elevating effect on chlorophyll fluorescence parameters, increased endogenous IAA content and decreased ABA content, and increased glutamate, serine, aspartate, and phenylalanine contents. However, cycloleucine significantly inhibited plant growth; plant apical dominance disappeared, plant height and dry and fresh weights decreased significantly, the development of abaxial leaf hairs was hindered, the net photosynthetic rate and Calvin cycle key enzyme activities were inhibited, the endogenous hormones IAA and ABA content decreased, and the conversion and utilization of glutamate, arginine, threonine, and glycine were affected. Combined with the experimental results and plant growth phenotypes, 20 mmol L− 1 cycloleucine significantly inhibited plant growth. In conclusion, 40 mmol L− 1 glycine betaine and 20 mmol L− 1 cycloleucine had different regulatory effects on plant growth and development.

show abstract

Section: Discussionmentioning

confidence: 66%

Effects of Exogenous Glycine Betaine and Cycloleucine on Photosynthetic Capacity, Amino Acid Composition, and Hormone Metabolism in Solanum melongena L

Niu

Zhang

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…1 ) which could be explained by the key role of GSH in scavenging of ROS. Change in the ratio of GSH/GSSG during the degradation of H 2 O 2 plays an important role in some redox signaling pathways 49 . GSH content decreased at later testing periods in 150 and 200 mg kg −1 Cd treatment, while GSSG content continued to increase.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical analysis reveals an antioxidant defense response in Lonicera japonica to cadmium-induced oxidative stress

Tang

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Cadmium (Cd), though potentially beneficial at lower levels to some plant species, at higher levels is a toxic metal that is detrimental to plant growth and development. Cd is also a carcinogen to humans and other contaminated plant consumers, affecting the kidneys and reducing bone strength. In this study we investigated responses of growth, chlorophyll content, reactive oxygen species levels, and antioxidant responses to Cd in honeysuckle leaves (Lonicera japonica Thunb.), a potential Cd hyperaccumulator. Results indicated that plant height, dry weight, leaf area, and chlorophyll content increased when honeysuckle was exposed to 10 mg kg−1 or 30 mg kg−1 Cd (low concentration). However, in response to 150 mg kg−1 or 200 mg kg−1 Cd (high concentration) these growth parameters and chlorophyll content significantly decreased relative to untreated control plant groups. Higher levels of superoxide radical (O2·−) and hydrogen peroxide (H2O2) were observed in high concentration Cd groups. The activities of ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), and glutathione reductase were enhanced with exposure to increasing levels of Cd. Additionally, the Ascorbate–Glutathione (AsA–GSH) cycle was activated for the removal of H2O2 in honeysuckle in response to elevated Cd. The Pearson correlation analysis, a redundancy analysis, and a permutation test indicated that proline and APX were dominant antioxidants for removing O2·− and H2O2. The antioxidants GSH and non-protein thiols (NPTs) also increased as the concentration of Cd increased.

show abstract

“…Pathogen‐induced SA accumulation dampened indole acetic acid (IAA) biosynthesis via increased photorespiratory H 2 O 2 in Arabidopsis , due to the inactivation of tryptophan synthetase (TSB) activity and decreased tryptophan (precursor for IAA biosynthesis) level (Yuan et al., 2017). Consistently, downregulation of photorespiratory H 2 O 2 increased IAA accompanied with increased TSB activity and tryptophan (Li et al., 2021). In this study, SA‐driven dissociation between GLO and CAT triggered a transient elevation in the photorespiratory H 2 O 2 (Figures 5 and 6), which may have mediated the inhibition of SA on IAA biosynthesis.…”

Section: Discussionmentioning

confidence: 72%

“…To construct transgenic rice, pOx‐ GFP‐GLO1 overexpression vectors were introduced into Cas9 ‐edited glo1 mutants (Li et al., 2021). Agrobacterium (EHA105)‐mediated transformation was used to generate hygromycin‐resistant T 0 lines.…”

Section: Methodsmentioning

confidence: 99%

“…The GLO activity assay was performed with 50 m m PBS (pH 7.8), 0.1 m m flavin mononucleotide (FMN), 2 m m phenol, 1 m m 4‐amino‐antipyrine, 5 units of horseradish peroxidase and 5 m m glycolate at 30°C. The reaction was initiated by adding the enzyme, and the absorbance at 520 nm was recorded for 1 min (Li et al., 2021). Protein content was determined using CBB G‐250.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Dynamic and fluctuating generation of hydrogen peroxide via photorespiratory metabolic channeling in plants

Liao

Huang

et al. 2022

The Plant Journal

Self Cite

View full text Add to dashboard Cite

SUMMARY The homeostasis of hydrogen peroxide (H2O2), a key regulator of basic biological processes, is a result of the cooperation between its generation and scavenging. However, the mechanistic basis of this balance is not fully understood. We previously proposed that the interaction between glycolate oxidase (GLO) and catalase (CAT) may serve as a molecular switch that modulates H2O2 levels in plants. In this study, we demonstrate that the GLO–CAT complex in plants exists in different states, which are dynamically interchangeable in response to various stimuli, typically salicylic acid (SA), at the whole‐plant level. More crucially, changes in the state of the complex were found to be closely linked to peroxisomal H2O2 fluctuations, which were independent of the membrane‐associated NADPH oxidase. Furthermore, evidence suggested that H2O2 channeling occurred even in vitro when GLO and CAT worked cooperatively. These results demonstrate that dynamic changes in H2O2 levels are physically created via photorespiratory metabolic channeling in plants, and that such H2O2 fluctuations may serve as signals that are mechanistically involved in the known functions of photorespiratory H2O2. In addition, our study also revealed a new way for SA to communicate with H2O2 in plants.

show abstract

Glycolate oxidase-dependent H2O2 production regulates IAA biosynthesis in rice

Cited by 21 publications

References 62 publications

Effects of Exogenous Glycine Betaine and Cycloleucine on Photosynthetic Capacity, Amino Acid Composition, and Hormone Metabolism in Solanum melongena L

Effects of Exogenous Glycine Betaine and Cycloleucine on Photosynthetic Capacity, Amino Acid Composition, and Hormone Metabolism in Solanum melongena L

Phytochemical analysis reveals an antioxidant defense response in Lonicera japonica to cadmium-induced oxidative stress

Dynamic and fluctuating generation of hydrogen peroxide via photorespiratory metabolic channeling in plants

Contact Info

Product

Resources

About