2012
DOI: 10.1105/tpc.111.093245
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Glycolate Oxidase Modulates Reactive Oxygen Species–Mediated Signal Transduction during Nonhost Resistance in Nicotiana benthamiana and Arabidopsis  

Abstract: In contrast to gene-for-gene disease resistance, nonhost resistance governs defense responses to a broad range of potential pathogen species. To identify specific genes involved in the signal transduction cascade associated with nonhost disease resistance, we used a virus-induced gene-silencing screen in Nicotiana benthamiana, and identified the peroxisomal enzyme glycolate oxidase (GOX) as an essential component of nonhost resistance. GOX-silenced N. benthamiana and Arabidopsis thaliana GOX T-DNA insertion mu… Show more

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Cited by 220 publications
(209 citation statements)
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“…An oxidative burst is a hallmark in nearly all plant responses to pathogen attack and plays an important signaling role in PCD (Torres, 2010). Different enzymatic sources of ROS have been described (Daudi et al, 2012;Ishiga et al, 2012;Rojas et al, 2012); however, the membrane-bound RBOH complex seems to play a central role in generating apoplastic ROS in PTI and ETI and possibly in amplifying ROS generated in different cellular compartments (Torres, 2010). The role of specific RBOH enzymes in pathogen-elicited PCD and resistance is complex, and they play distinct roles depending on the pathogen and the plant species.…”
Section: Discussionmentioning
confidence: 99%
“…An oxidative burst is a hallmark in nearly all plant responses to pathogen attack and plays an important signaling role in PCD (Torres, 2010). Different enzymatic sources of ROS have been described (Daudi et al, 2012;Ishiga et al, 2012;Rojas et al, 2012); however, the membrane-bound RBOH complex seems to play a central role in generating apoplastic ROS in PTI and ETI and possibly in amplifying ROS generated in different cellular compartments (Torres, 2010). The role of specific RBOH enzymes in pathogen-elicited PCD and resistance is complex, and they play distinct roles depending on the pathogen and the plant species.…”
Section: Discussionmentioning
confidence: 99%
“…DAB staining has been often used to visualise the generation of H 2 O 2 in planta (Asai et al, 2010;Dubreuil-Maurizi et al, 2010;Kobayashi et al, 2012;L'Haridon et al, 2011;Liao et al, 2012;Rojas et al, 2012;Simon et al, 2013;Torres et al, 2005;Yokawa et al, 2011;Zhang et al, 2012). The Amplex Red hydrogen peroxide/peroxidase activity assay also allows quantifying H 2 O 2 concentrations and consists in a non-fluorescent molecule that is oxidised by H 2 O 2 and becomes fluorescent in presence of peroxidase.…”
Section: Detection Of Rosmentioning
confidence: 99%
“…Four discs of 6-mm diameter were collected per sample at the indicated time point. Leaf samples were ground, subjected to serial dilution, plated on King's B agar medium supplemented with appropriate antibiotics, and incubated at 28°C for 2 d for bacterial colony counting, as previously described (Rojas et al, 2012). For Pst DC3000-induced HR cell death assays, the leaves of GAPC-silenced and control N. benthamiana plants were inoculated with Pst DC3000 at 10 7 cfu/mL, and HR was observed at 15 hpi.…”
Section: Bacterial Strain Growthmentioning
confidence: 99%