Glycolysis and Its Metabolic Engineering Applications

Abstract

IntroductionMicroorganisms play a pivotal role in the degradation of complex carbon sources in nature and could survive in different conditions due to the genetically coined cellular metabolism. Cellular metabolism of microorg anisms is defined as the sum of biochemical processes that involves the conversion of environmental nutrients into simple biosynthetic building blocks and energy in the cells. Within these metabolic processes, catabo lism is responsible for breakdown of complex molecules into simple molecules, producing energy, reducing power, and precursor intermedi ates for other metabolic processes like anabolism. The central catabolic pathways include Embden-Meyerhof-Parnas (EMP) pathway, pentose phosphate (PP) pathway, Entner-Doudoroff (ED) pathway, and the tricar boxylic acid (TCA) cycle. The EMP pathway, also known as glycolysis

Time-resolved, deuterium-based fluxomics uncovers the hierarchy and dynamics of sugar processing byPseudomonas putida

“…Yet, understanding glucose catabolism in non-canonical bacterial hosts [5] is of paramount interest to engineer efficient sugar consumption pathways [6]-an aspect that has largely relied on manipulating the EMP route thus far. Indeed, the Entner-Doudoroff (ED) and the pentose phosphate (PP) pathways (or variations thereof) prevail as the main sugar catabolic route in microorganisms that live in diverse, often-changing environmental niches [7][8][9][10][11].…”