Glycolysis in Normal and in Leukemic Blood

Falcon-Lesses, Mark

doi:10.1001/archinte.1927.00130030097008

Cited by 12 publications

(4 citation statements)

References 5 publications

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“…of blood from the arm vein under sterile precautions, no tourniquet being used in order to avoid stasis. The blood was discharged into a large test tube, mixed with heparin, and kept in a warm room at a temperature of 370 C. Heparin was used as an anti-coagulant as it was shown by Falcon-Lesses (3) to have no appreciable effect on the rate of glycolysis.…”

Section: Methodsmentioning

confidence: 99%

Glycolysis in the Blood of Patients With Pernicious Anemia

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1933

J. Clin. Invest.

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Following the discovery by Warburg that tumor cells have quantitative and qualitative differences from those of normal tissues with regard to their carbohydrate metabolism, many investigators have demonstrated that the method of glycolysis was most satisfactory for the study of the metabolism of the blood cells. Maclean and Weir (1) pointed out the role played by the different blood elements in glycolysis, and showed that the erythrocytes were a vital factor in the sugar consumption of normal blood. Inasmuch as the red blood cells play an important part in the glycolytic activity of normal blood, and since pernicious anemia is a disease primarily involving the red cells (both number and type), a study of the glycolysis of the blood in this malady has been undertaken for further investigation of the metabolism of the red cells. METHODSBlood sugar determinations were made from the blood of nine patients with pernicious anemia before treatment and at the height of the reticulocyte response, following ventriculin therapy. Red cell, white cell and reticulocyte counts were made on corresponding days.The blood sugar values were calculated according to the micro-method of Folin and Wu (2). All the estimations were made from blood samples obtained before breakfast at 8 A.M. The usual procedure was to withdraw 10 to 15 cc. of blood from the arm vein under sterile precautions, no tourniquet being used in order to avoid stasis. The blood was discharged into a large test tube, mixed with heparin, and kept in a warm room at a temperature of 370 C. Heparin was used as an anti-coagulant as it was shown by Falcon-Lesses (3) to have no appreciable effect on the rate of glycolysis.The blood sugar content was determined immediately after the removal of the specimen from the patient, and then at hourly intervals for 5 hours to estimate the glycolytic activity. The red blood cell and white blood cell counts were made with U. S. Bureau of Standards pipettes and Neubauer-Levy counting chambers. Reticulocyte estimations were made from brilliant cresyl-blue film preparations. 583

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Section: Methodsmentioning

confidence: 99%

Glycolysis in the Blood of Patients With Pernicious Anemia

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1933

J. Clin. Invest.

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“…Mackenzie (1915), Katayama (1926) and Falcon-Lesses (1927) found that slight bacterial contamination did not affect the glycolytic rate.…”

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confidence: 97%

Studies of Blood Glycolysis

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1932

J. Clin. Invest.

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The series of experiments presented in this paper demonstrate certain relationships in the progressive changes of sugar and phosphorus observed in defibrinated blood incubated in vitro at 370 C. through periods of from ten to twenty-four hours. The accompanying figures illustrate a pattern of these changes which may be regarded as characteristic of normal bloods and bloods of normal animals rendered hyperglycemic and hypoglycemic. Similar studies of glycolysis and phosphorus relationships observed in blood samples from children with a variety of diseases will be presented in the succeeding paper.Most of the evidence that phosphorus plays an essential r8le in the intermediary carbohydrate metabolism of the animal body is based upon observations of shifts of phosphorus in the body and fluctuations in the excretion of phosphorus which accompany the storage and burning of sugar. This subject has been recently reviewed by Peters and Van Slyke (1931). It is generally believed that there is formation of carbohydrate-phosphoric acid compounds at certain stages in the processes of storage (as in the synthesis of glycogen) and burning of sugar, but the actual isolation of such compounds from animal tissues has proved difficult and the evidence that such compounds exist in the animal body is for the most part indirect. Goodwin and Robison (1924) reported the isolation of two phosphoric esters from the blood; one of these esters reduced Fehling's solution. The existence of hexose-phosphoric acid compounds in yeasts was demonstrated by Harden and his collaborators (1905-1923), Robison (1922), Levene and Raymond (1928, 1929), and the r6le of phosphorus in alcoholic fermentation of sugar by yeasts was clearly defined by Harden's studies (1923). The manner in which phosphorus enters into the splitting of sugar by yeast enzymes is better understood than are the processes by which sugar is utilized in the animal body, but certain steps of the chemical mechanisms in the respective organisms are probably closely analogous and much that has been learned from the one has been profitably applied by a number of investigators in studying the other.The earliest suggestion that phosphorus might be concerned in blood glycolysis appears to have been made by Rona and Doblin (1911), who seized I An abstract of this paper was read at a meeting of the American Pediatric Society, Montreal, June 18, 1930. 555

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“…The significance of the rate of blood glycolysis as it occurs in health and in disease has been the subject of a great many investigations which are cited in the articles by John (1925), Falcon-Lesses (1927) and Barer (1931). The rate of blood glycolysis is fairly constant in health but varies greatly in certain conditions of disease, the most rapid rates having been observed in leukemia (Falcon-Lesses (1927)).…”

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confidence: 99%

“…The rate of blood glycolysis is fairly constant in health but varies greatly in certain conditions of disease, the most rapid rates having been observed in leukemia (Falcon-Lesses (1927)). Acceleration of the glycolytic rate has been observed also in diabetes, in heart disease, in nephritis, in septicemia, and in other unrelated conditions.…”

mentioning

confidence: 99%

Studies of Blood Glycolysis

Guest¹

1932

J. Clin. Invest.

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Glycolysis in Normal and in Leukemic Blood

Cited by 12 publications

References 5 publications

Glycolysis in the Blood of Patients With Pernicious Anemia

Glycolysis in the Blood of Patients With Pernicious Anemia

Studies of Blood Glycolysis

Studies of Blood Glycolysis

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