Glycolytic Activity of the Cornea

deROETTH, Andrew

doi:10.1001/archopht.1951.01700010144002

Cited by 27 publications

(2 citation statements)

References 14 publications

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“…The low level of aerobic glucose metabolism in the corneal epithelium (19,61,62), operating primarily via the hexosemonophosphate shunt, correlates well with the scarcity and internal simplicity of the mitochondria of the rabbit corneal epithelium described in the present study.…”

Section: Figure 15supporting

confidence: 86%

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Studies on the Cornea

Kaye

Pappas

1962

The Journal of Cell Biology

165

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Physiological studies have demonstrated that ions, as well as large molecules such as hemoglobin or fluorescein, can diffuse across and within the cornea. Most of the substrates for corneal metabolism are obtained from aqueous humor filling the anterior chamber. In order to receive its nutrients and in order to maintain its normal conditions of hydration, the avascular cornea must transport relatively large amounts of solute and solvent across the cellular layers which cover this structure. It has been suggested in the past that there may be a morphological basis for the transport of large amounts of solvents and solutes by cells by the mechanism of pinocytosis. The use of electron-opaque markers to study fluid movements at the electron microscope magnification level was described by Wissig (29). The present study describes the fine structure of the normal rabbit cornea and the pathways of transport of colloidal particles by the cornea in vivo. Rabbit corneas were exposed in vivo to suspensions of saccharated iron oxide, thorium dioxide, or ferritin by injection of the material into the anterior chamber. In other experiments thorium dioxide or saccharated iron oxide was injected into the corneal stroma, producing a small bleb. Particles presented at the aqueous humor surface of the rabbit corneal endothelium are first attached to the cell surface and then pinocytosed. It appears that the particles are carried around the terminal bar by an intracellular pathway involving the pinocytosis of the particles and their subsequent transport in vesicles to the lateral cell margin basal to the terminal bar. Particles introduced at the basal surface of the endothelium (via blebs in the corneal stroma) are apparently carried through the endothelial cells in membrane-bounded vesicles without appearing in the intercellular space. There appears to be free diffusion of these particles through Descemet's membrane and the corneal stroma. The stromal cells take up large quantities of the particles when blebs are injected into the stroma.

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Section: Figure 15supporting

confidence: 86%

“…De Roetth (61) reported that the bovine corneal epithelium did not appear to have aerobic glycolysis when lactate and CO2 production in the whole cornea was compared with that in the cornea denuded of its epithelium. These results were essentially confirmed by Langham (19).…”

Section: Figure 15mentioning

confidence: 99%