Glycolytic and gluconeogenic enzyme activities in parenchymal and non-parenchymal cells from mouse liver

Abstract

Parenchymal cells contain both glucose 6-phosphatase and fructose 1,6-diphosphatase. The non-parenchymal fraction appears to contain fructose 1,6-diphosphatase, but is devoid of glucose 6-phosphatase. 8. No aldolase A was detectable in the whole liver. Aldolase B occurs in both parenchymal and non-parenchymal tissue. 9. Parenchymal cells prepared by mechanical disruption ofmouse liver with 20 % polyvinyl alcohol exhibit a similar enzyme profile to those prepared enzymically. 10. The methodology involved in the preparation of isolated liver cells is discussed. The importance of the measurement of several parameters as criteria for establishing the viability of parenchymal cells is stressed. 11. The metabolic implications of the results in the present study are discussed.

Undifferentiated Patterns of Key Carbohydrate-Metabolizing Enzymes in Injured Livers. I. Acute Carbon-Tetrachloride Intoxication of Rat

“…Before drawing this conclusion, the possibility that the altered enzyme pattern is merely a reflexion of the change in cell population resulting from necrosis and regeneration of hepatocytes and cell infiltration is to be con sidered, since the differential distribution of some enzymes in hepatic tissue has been reported to exist [43][44][45][46]. However, this is not likely to be the cause.…”

Laminin and fibronectin in cell adhesion: enhanced adhesion of cells from regenerating liver to laminin.

“…The liver mass that consists of nonparenchymal material only contributes 10-20% of the liver at any given stage ofregeneration (31, 32) whereas the cell suspensions we prepared represented 50-60% of the original liver mass. Moreover, the collagenase perfusion method gives cell preparations containing 90-99% hepatocytes (25,(33)(34)(35). Finally, 90% of the cells we obtained showed immunofluorescence for albumin, a known marker for hepatocytes in normal and regenerating liver (36,37).…”

The Role of Futile Cycles in the Regulation of Carbohydrate Metabolism in the Liver

“…This conclusion had been previously reached by several groups of workers (Sapag-Hagar et al, 1969;Crisp and Pogson, 1972), although other investigators (Morrison, 1967;Werner et al, 1972;Bonney et al, 1973) have obtained preparations of hepatocytes that still contained hexokinase.…”

“…Type Lpyruvate kinase is restricted to hepatocytes, whereas type A is confined to nonparenchymal cells (Crisp and Pogson, 1972;Van Berkel et al, 1972). The major isoenzyme of chicken liver is the A (or K) type (Eigenbrodt and Schoner, 1977a).…”