Glycomic profiling of carcinoembryonic antigen isolated from human tumor tissue

Huang, Chuncui; Zhan, Tiancheng; Liu, Yaming; Li, Qianqian; Wu, Hongmei; Ji, Dengbo; Li, Yan

doi:10.1186/s12014-015-9088-3

Cited by 26 publications

(18 citation statements)

References 28 publications

(22 reference statements)

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“…A continuous increase in serum CEA is typically correlated with disease progression ( 75 ). Also, analytical glycoprofiling of circulating cancer-associated CEA has revealed significantly increased expression of type I and type II Lewis antigens compared to CEA from healthy individuals ( 76 ). This may possibly explain the current focus on the glycosylation status of various cancer-related protein biomarkers and on the implementation of more fucosylated epitopes (e.g., type II Lewis antigens) in the field of cancer biomarker research.…”

Section: Lewis Antigens As Cancer Biomarkersmentioning

confidence: 99%

Fucosylated Antigens in Cancer: An Alliance toward Tumor Progression, Metastasis, and Resistance to Chemotherapy

et al. 2018

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Aberrant glycosylation of tumor cells is recognized as a universal hallmark of cancer pathogenesis. Overexpression of fucosylated epitopes, such as type I (H1, Lewisa, Lewisb, and sialyl Lewisa) and type II (H2, Lewisx, Lewisy, and sialyl Lewisx) Lewis antigens, frequently occurs on the cancer cell surface and is mainly attributed to upregulated expression of pertinent fucosyltransferases (FUTs). Nevertheless, the impact of fucose-containing moieties on tumor cell biology is not fully elucidated yet. Here, we review the relevance of tumor-overexpressed FUTs and their respective synthesized Lewis determinants in critical aspects associated with cancer progression, such as increased cell survival and proliferation, tissue invasion and metastasis, epithelial to mesenchymal transition, epithelial and immune cell interaction, angiogenesis, multidrug resistance, and cancer stemness. Furthermore, we discuss the potential use of enhanced levels of fucosylation as glycan biomarkers for early prognosis, diagnosis, and disease monitoring in cancer patients.

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Section: Lewis Antigens As Cancer Biomarkersmentioning

confidence: 99%

Fucosylated Antigens in Cancer: An Alliance toward Tumor Progression, Metastasis, and Resistance to Chemotherapy

et al. 2018

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“…All tissue homogenates were incubated and then centrifuged at 15,000 g for 15 min, the supernatant was kept at – 80 °C. Subsequently, the concentration of CEA was determined using Human Carcinoembryonic Antigen (CEA) ELISA Kit [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

“…Recently, we examined glycans in colorectal carcinoma tissue samples, and identified 61 N -glycoforms present on the surface of CEA. In one of our recent studies, we showed that the composition of the glycans associated with CEA displays a considerable heterogeneity [ 19 ]. Saeland et al [ 20 ] compared CEA glycosylation patterns of normal and colorectal cancer tissues and found that Lewis X, Lewis Y, mannose and branched N -glycans are increased in tumor-associated CEA.…”

Section: Introductionmentioning

confidence: 99%

Glycan analysis of colorectal cancer samples reveals stage-dependent changes in CEA glycosylation patterns

et al. 2018

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BackgroundCarcinoembryonic antigen (CEA) is a glycoprotein associated with colorectal cancer (CRC). While the functions of its gene and protein have been fully characterized, its post-translational modifications in the context of CRC development remain undefined.MethodsTo show the correlation between the different stages of CRC development and changes in the glycosylation patterns of CEA, we analyzed CEA in tumor tissues (CEA-T) and paired tumor-adjacent normal tissues (CEA-A) from 53 colorectal cancer patients using a high-density lectin microarray containing 56 plant lectins.ResultsWe detected higher expression levels of fucose, mannose and Thomsen–Friedenreich antigen, and lower expression levels of N-acetylgalactosamine, N-acetylglucosamine, galactose, branched and bisecting N-glycans on CEA in the tumor tissues relative to the tumor-adjacent normal tissues. Furthermore, a combinatorial assessment of 9 lectins is sufficient to distinguish CRC tumor tissues from tumor-adjacent normal tissues with 83% sensitivity and ~ 90% specificity. Moreover, the levels of N-acetylgalactosamine, mannose, galactose, N-acetylglucosamine on CEA showed a downward trend after first experiencing an increase at Stage II with the stages of CRC.ConclusionsOur insights into the changing CEA glycosylation patterns and their role in the development of CRC highlight the importance of glycan variants on CEA for early clinical detection and staging of CRC.Electronic supplementary materialThe online version of this article (10.1186/s12014-018-9182-4) contains supplementary material, which is available to authorized users.

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“… 9 It was reported that the levels of serum TMs showed better diagnostic values in partial malignant tumors, for example, α-fetoprotein (AFP) and prostate-specific antigen became the specific TMs of liver cancer and prostate cancer, respectively. 10 , 11 It has been demonstrated that squamous cell carcinoma antigen (SCCAg), cytokeratin 19 fragment (Cyfra21-1), and carcinoembryonic antigen (CEA) were significantly higher in cervical squamous cell carcinoma, small cell lung cancer, and colorectal carcinoma respectively; 12 – 14 the levels of serum Cyfra21-1 and SCCAg were significantly higher in partial patients with head and neck squamous cell carcinoma, 15 , 16 suggesting that these serum TMs might have better clinical value in the screening and diagnosis of the tumor. So far, there is still a lack of any simple or effective method to screen and diagnose patients with OSCC/OPSCC.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic values of serum tumor markers Cyfra21-1, SCCAg, ferritin, CEA, CA19-9, and AFP in oral/oropharyngeal squamous cell carcinoma

Yuan

Yang

Tang

et al. 2016

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BackgroundAt present, the research on serum tumor markers in the early diagnosis of malignant tumors has aroused widespread concern. The aim of this study was to investigate the diagnostic values of serum tumor markers cytokeratin 19 fragment (Cyfra21-1), squamous cell carcinoma antigen (SCCAg), ferritin, carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9), and α-fetoprotein (AFP) for patients with oral/oropharyngeal squamous carcinoma (OSCC/OPSCC).MethodsOne hundred and sixty-nine cases of patients with OSCC/OPSCC as the experimental group, 86 cases of oral benign tumor patients as the control group, and 30 cases of healthy people as the normal control group were studied. The levels of serum Cyfra21-1, SCCAg, ferritin, CEA, CA19-9, and AFP were measured using electrochemiluminescence immunoassay.ResultsThe levels of serum Cyfra21-1, SCCAg, ferritin, and CEA in patients with OSCC/OPSCC were significantly higher than those of benign tumor and healthy control group (P<0.05). The levels of CA19-9 and AFP showed no significant difference between patients with OSCC/OPSCC, benign tumor, and healthy group (P>0.05). The level of serum Cyfra21-1 in patients with early OSCC/OPSCC (stage I + II) was significantly higher than that of benign tumor and healthy control group (P<0.05). However, the levels of serum SCCAg, ferritin, CEA, CA19-9, and AFP showed no significant difference between patients with early OSCC/OPSCC, benign tumor, and healthy control group (P>0.05). The levels of serum Cyfra21-1, SCCAg, ferritin, and CEA in the middle-late stage of patients with OSCC/OPSCC (stage III + IV) were significantly higher than those of patients with the early OSCC/OPSCC, benign tumor, and healthy control group (P<0.05). The diagnostic cutoff levels of Cyfra21-1, SCCAg, ferritin, and CEA were 2.17, 0.72, 109.95, and 1.99 ng/mL, respectively. The sensitivities were 60.36%, 73.37%, 81.66%, and 66.27%, respectively. The specificities were 81.03%, 68.10%, 40.52%, and 61.21%, respectively.ConclusionCyfra21-1, SCCAg, ferritin, and CEA had diagnostic values for patients with OSCC/OPSCC. Meanwhile, Cyfra21-1 had better early diagnostic value for patients with OSCC/OPSCC.

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Glycomic profiling of carcinoembryonic antigen isolated from human tumor tissue

Cited by 26 publications

References 28 publications

Fucosylated Antigens in Cancer: An Alliance toward Tumor Progression, Metastasis, and Resistance to Chemotherapy

Fucosylated Antigens in Cancer: An Alliance toward Tumor Progression, Metastasis, and Resistance to Chemotherapy

Glycan analysis of colorectal cancer samples reveals stage-dependent changes in CEA glycosylation patterns

Diagnostic values of serum tumor markers Cyfra21-1, SCCAg, ferritin, CEA, CA19-9, and AFP in oral/oropharyngeal squamous cell carcinoma

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