Glycomyces sediminimaris sp. nov., a new species of actinobacteria isolated from marine sediment

Abstract

A novel Glycomyces strain, designated as MH2460, was isolated from marine sediment collected from 12 m depth in Rostami seaport, Bushehr Province in Iran. On International Streptomyces Project 2 medium it produced branching substrate hyphae that developed into a large number of irregularly shaped spores in 8 days. It showed optimal growth at 25-35 °C, pH 6.0-8.0 and in salinity between 2.5-5 % (w/v) NaCl. Chemotaxonomic and molecular characteristics of the isolate matched descriptions for members of the genus Glycomyces. Whole-cell hydrolysates of strain MH2460 contained meso-diaminopimelic acids along with glucose, ribose and small traces of xylose and galactose. The phospholipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides as well as two unidentified phosphoglycolipids, one unidentified phospholipid and an unidentified aminolipid. The predominant menaquinones were MK-11(H4) and MK-10(H4). The fatty-acid pattern was mainly composed of anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C16 : 0. The strain belongs to the genus Glycomyces based on 16S rRNA gene sequence with the highest pairwise sequence identity (98.3 %) with Glycomyces phytohabitans KLBMP 1483. The DNA-DNA hybridization value showed 53.9±2.7 % identity when MH2460 was compared to this reference strain. The G+C content of the DNA was 70.2 mol%. Based on phenotypic, biochemical, chemotaxonomic and genotypic features, strain MH2460 (DSM 103727=UTMC 2460=NCCB 100631) is considered to represent a novel species of the genus Glycomyces, for which the name Glycomyces sediminimaris is proposed.

Anti-microfouling Activity of Glycomyces sediminimaris UTMC 2460 on Dominant Fouling Bacteria of Iran Marine Habitats

“…The sequence of its 16S rRNA was deposited in the GenBank database under accession number KU1741966. The strain has been identified as a new species in the genus under the epithet of Glycomyces sediminimaris UTMC 2460, using a polyphasic assessment (Mohammadipanah et al, 2018).…”

Natronoglycomyces albus gen. nov., sp. nov, a haloalkaliphilic actinobacterium from a soda solonchak soil

“…§ §Data for G. halotolerans [23], G. tarimensis [22] and G. sediminimaris [26] determined in the culture media with pH buffering systems. || ||Data for S. cavernae determined in the culture medium with pH buffering systems [27].…”

“… ‡‡Data for G . lacisalsi determined in the culture medium with pH buffering systems [24]; (note: indicated growth maximum pH seems questionable as this value is higher than the confirmed prokaryotic maximum [60]). §§Data for G. halotolerans [23], G. tarimensis [22] and G. sediminimaris [26] determined in the culture media with pH buffering systems. || ||Data for S. cavernae determined in the culture medium with pH buffering systems [27]. *These values are unrealistic, since the media used were not properly buffered.…”

“… §§Data for G. halotolerans [ 23 ], G. tarimensis [ 22 ] and G. sediminimaris [ 26 ] determined in the culture media with pH buffering systems. …”

“…This paper presents the results of taxonomic study of strain ACPA22 T assigned to a new genus and species of the family Glycomycetaceae (order Glycomycetales) [11][12][13][14][15], which currently includes five genera, namely Glycomyces, Stackebrandtia, Haloglycomyces, Salininema and Salilacibacter [15][16][17][18][19][20][21]. Several species of the above genera originate from high-salt habitats and/or show resistance to saline and alkaline conditions [19][20][21][22][23][24][25][26][27].…”