Glycopeptide antibiotic discovery in the genomic era

Synthetic Biology Facilitates Semisynthetic Development of Type V Glycopeptide Antibiotics Targeting Vancomycin-Resistant Enterococcus

“…The seed culture was then inoculated into 650 mL SAM medium (5% inoculum) in a 2.8 L Thomson ULTR YIELD flask (×20), growing for 8 days at 30 °C. Rimomycin A was then purified from the harvested mycelium using an isolation procedure designed for Type V GPAs described in our previous report . Yield: 600 mg/L (24-well plate format).…”

“…Rimomycin A was then purified from the harvested mycelium using an isolation procedure designed for Type V GPAs described in our previous report. 40 Yield: 600 mg/L (24-well plate format).…”

糖肽抗生素天然产物研究进展

“…不同于Ⅰ-Ⅳ型 GPA, 在Ⅴ型 GPA 基因簇中并未发现 vanRSHAX 或 vanRSCXYT 抗性基因簇 [7] . [110] . 以 Complestatin 基因簇为例, comU 和 comV 分别为 vanS 和 vanR 同源基因, 在 comU 上游存在由 comQRST 四个未知基因形成的转录本.…”

“…FtsEX 对于细菌细胞的分裂至关重要, 它有助于分裂体的建立并在肽聚糖合成和水解过程 中起激活和调节功能 [111] . ComQRST 可能参与调控 Complestatin 对链霉菌细胞壁肽聚糖降解途 径的抑制从而为菌体提供抗性, 但相关机制还在研究中 [110] .…”