Glycopolymer monoliths for affinity bioseparation of proteins in a continuous-flow system: glycomonoliths

Seto, Hirokazu; Shibuya, Makoto; Matsumoto, Hikaru; Hoshino, Yu; Miura, Yoshiko

doi:10.1039/c6tb02930b

Cited by 11 publications

(4 citation statements)

References 59 publications

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“…Pressure losses at the steady state proportionally increased with increasing flow rates (Figure S7b). The k D of MPGs, porous glass membrane, and silica particles were estimated to be around 10 –14 –10 –13 m 2 (Figure ), which were comparable to those of commercially available membrane filters with pore sizes ranging from 1000 to 10 000 nm . After loading of Pd(0), the k D was in almost of the same order as that of the original supports (Figures and S7c).…”

Section: Resultssupporting

confidence: 55%

“…The k D of MPGs, porous glass membrane, and silica particles were estimated to be around 10 −14 −10 −13 m 2 (Figure 3), which were comparable to those of commercially available membrane filters with pore sizes ranging from 1000 to 10 000 nm. 39 After loading of Pd(0), the k D was in almost of the same order as that of the original supports (Figures 3 and S7c). Pd/C also showed almost the same permeability (2.4 × 10 −14 m 2 ) as that of porous glass membrane and silica particles.…”

Section: Acs Omegamentioning

confidence: 68%

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Macroporous Gel with a Permeable Reaction Platform for Catalytic Flow Synthesis

et al. 2017

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We mimic a living system wherein target molecules permeate through capillary and cells for chemical transformation. A monolithic porous gel (MPG) was easily prepared by copolymerization of gel matrix, tertiary amine, and cross-linking monomer in one-step synthesis. Interconnected capillaries existed in the MPG, enabling flow application with high permeability. Because the capillaries were constituted of polymer gel, Pd(0)-loaded MPG provided another permeable pathway to substrates in a gel network, contributing to its much high turnover number after 30 days of use, compared with that of Pd(0)-loaded inorganic supports. Interestingly, the gel network size of the MPG influenced the catalytic frequency. Diffusivities of the substrates and product in the gel networks increased with increasing network sizes in relation to catalytic activities. The MPG strategy provides a universal reactor design in conjunction with a practical process and precisely controlled reaction platform.

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Section: Resultssupporting

confidence: 55%

Section: Acs Omegamentioning

confidence: 68%

Macroporous Gel with a Permeable Reaction Platform for Catalytic Flow Synthesis

et al. 2017

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“…According to Darcy's law, the bed permeability (K) of the BOPM is 2.56 × 10 −12 m 2 , which is 1−2 orders of magnitude higher than that of reported macroporous monoliths. 15,20,39 The low backpressure and high permeability of the BOPM can be ascribed to the presence of interconnected flow-through pores in the BOPM, promising high mass transfer rate during chromatographic separation. Con A is a well-known saccharide-binding protein that can be specifically adsorbed on glycopolymers.…”

Section: Chemical Composition Analysis Ofmentioning

confidence: 99%

“…Con A is a well-known saccharide-binding protein that can be specifically adsorbed on glycopolymers. 39 Figure 8b shows the chromatograms of the BSA and Con A mixture on the BOPM in the HILIC mode. BSA did not adsorb to the column but appears as a breakthrough peak owing to the hydrophilic surface of the BOPM.…”

Section: Chemical Composition Analysis Ofmentioning

confidence: 99%

Synthesis of Bimodal Open-Porous Polystyrene Monoliths with Glycopolymer Surfaces for High-Speed Protein Chromatography

Lin

Liu

et al. 2021

ACS Appl. Polym. Mater.

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Conventional polystyrene (PS) monoliths are rarely used for separation of biomacromolecules due to their heterogeneous skeletons and hydrophobic surfaces. In this work, highpermeable and bimodal open-porous polystyrene monoliths (BOPMs) with homogeneous skeletons and glycopolymer surfaces were prepared by reversible addition−fragmentation chain transfer (RAFT) polymerization via an emulsion-templating method. The stable and bicontinuous medium-internal phase emulsion (MIPE) was stabilized by the cooperative assembly of two surfactants, that is, a self-made amphiphilic diblock glycopolymer (ADG) in the oil phase and Pluronic F127 (PF127) in the water phase. It was found that the higher hydrophilic−lipophilic balance (HLB) value of ADGs needs to match the higher concentration of PF127 to promise the homogeneous structure of the bicontinuous MIPE. A possible mechanism was proposed based on the hydrogen bonding interaction. Using the MIPE as a template, BOPMs were successfully synthesized by RAFT emulsion polymerization. The effects of the HLB value and adding amount of ADGs on the morphology of the BOPM were also studied. There are two sets of pores in the BOPM determined by mercury porosimetry, that is, gigapores (200−7000 nm) and mesopores (20−100 nm). Taking advantage of glycopolymer surfaces of the BOPM, glycoprotein can be directly separated at a mobile-phase velocity of up to 1806 cm/h in the hydrophilic interaction liquid chromatography mode. The BOPM as prepared shows good permeability, mechanical stability, and biocompatibility, which is an ideal support in high-speed protein chromatography, cell culture, and enzyme immobilization.

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Monolith columns for liquid chromatographic separations of intact proteins: A review of recent advances and applications

Lynch

Ren

Beckner

et al. 2019

Analytica Chimica Acta

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Glycopolymer monoliths for affinity bioseparation of proteins in a continuous-flow system: glycomonoliths

Abstract: Macroporous materials, called glycomonoliths, were prepared from saccharide-containing monomers, and applied for affinity bioseparation of proteins in a continuous-flow system.

Cited by 11 publications

References 59 publications

Macroporous Gel with a Permeable Reaction Platform for Catalytic Flow Synthesis

Macroporous Gel with a Permeable Reaction Platform for Catalytic Flow Synthesis

Synthesis of Bimodal Open-Porous Polystyrene Monoliths with Glycopolymer Surfaces for High-Speed Protein Chromatography

Monolith columns for liquid chromatographic separations of intact proteins: A review of recent advances and applications

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