Glycosaminoglycan content of a mineralized collagen scaffold promotes mesenchymal stem cell secretion of factors to modulate angiogenesis and monocyte differentiation

Dewey, Maynard M.; Kolliopoulos, Vasiliki; Ngo, Mai T.; Harley, Brendan A.C.

doi:10.1101/2021.03.23.436487

Cited by 2 publications

(2 citation statements)

References 80 publications

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“…It is also essential to consider various versatile molecules within the native ECM, such as GAGs (Dewey et al, 2021) and VEGF (Liang et al, 2001), for promoting vasculogenesis. GAG chains are attached to proteoglycans which, based on the physicochemical characteristics of the glycosaminoglycan component, provide hydration and swelling capacities that allow tissues to withstand compressional forces (Yanagishita, 1993).…”

Section: Future Directions For Decelluarizing Vesselsmentioning

confidence: 99%

Decellularized blood vessel development: Current state-of-the-art and future directions

Wang

Chan

Corridon

2022

Front. Bioeng. Biotechnol.

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Vascular diseases contribute to intensive and irreversible damage, and current treatments include medications, rehabilitation, and surgical interventions. Often, these diseases require some form of vascular replacement therapy (VRT) to help patients overcome life-threatening conditions and traumatic injuries annually. Current VRTs rely on harvesting blood vessels from various regions of the body like the arms, legs, chest, and abdomen. However, these procedures also produce further complications like donor site morbidity. Such common comorbidities may lead to substantial pain, infections, decreased function, and additional reconstructive or cosmetic surgeries. Vascular tissue engineering technology promises to reduce or eliminate these issues, and the existing state-of-the-art approach is based on synthetic or natural polymer tubes aiming to mimic various types of blood vessel. Burgeoning decellularization techniques are considered as the most viable tissue engineering strategy to fill these gaps. This review discusses various approaches and the mechanisms behind decellularization techniques and outlines a simplified model for a replacement vascular unit. The current state-of-the-art method used to create decellularized vessel segments is identified. Also, perspectives on future directions to engineer small- (inner diameter >1 mm and <6 mm) to large-caliber (inner diameter >6 mm) vessel substitutes are presented.

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Section: Future Directions For Decelluarizing Vesselsmentioning

confidence: 99%

Decellularized blood vessel development: Current state-of-the-art and future directions

Wang

Chan

Corridon

2022

Front. Bioeng. Biotechnol.

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“…After sterilization, all subsequent steps proceeded using aseptic techniques. Sterile scaffolds were then hydrated and crosslinked using previously described EDC-NHS chemistry [11,[34][35][36][37]. Briefly, scaffolds were soaked in 100% ethanol, then washed multiple times in phosphate buffered saline (PBS), followed by EDC-NHS crosslinking.…”

Section: Sterilization Hydration Crosslinking and Creation Of Amnion/...mentioning

confidence: 99%

Soluble extracts from amnion and chorion membranes improve hMSC osteogenic response in a mineralized collagen scaffold

Kolliopoulos

Dewey

Polanek

et al. 2022

Preprint

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Craniomaxillofacial (CMF) bone injuries present a major surgical challenge and cannot heal naturally due to their large size and complex topography. Approximately 26% of injured Iraq war veterans sustained CMF injuries in the form of blast wounds, and 0.1% of births involve CMF defects like cleft palate. We previously developed a class of mineralized collagen scaffolds designed to mimic native extracellular matrix (ECM) features of bone. These scaffolds induce in vitro human mesenchymal stem cell (hMSC) osteogenic differentiation and in vivo bone formation without the need for exogenous osteogenic supplements. Here, we seek to enhance cellular bioactivity and osteogenic activity via inclusion of placental-derived products in the scaffold architecture. The amnion and chorion membranes are distinct components of the placenta that individually have displayed anti-inflammatory, immunogenic, and osteogenic properties. They represent a potentially powerful compositional modification to the mineralized collagen scaffolds to improve bioactivity. Here we examine introduction of the placental-derived amnion and chorion membranes or soluble extracts derived from these membranes into the collagen scaffolds, comparing the potential for these modifications to improve hMSC osteogenic activity. We report structural analysis of the scaffolds via mechanical compression testing, imaging via scanning electron microscopy (SEM), and assessments of various metrics for osteogenesis including gene expression (Nanostring), protein elution (ELISA), alkaline phosphatase (ALP) activity, inductively coupled plasma mass spectrometry (ICP) for mineralization, and cell viability (AlamarBlue). Notably, a post fabrication step to incorporate soluble extracts from the amnion membrane induces the highest levels of metabolic activity and performs similarly to the conventional mineralized collagen scaffolds in regard to mineral deposition and elution of the osteoclast inhibitor osteoprotegerin (OPG). Together, these findings suggest that mineralized collagen scaffolds modified using elements derived from amnion and chorion membranes, particularly their soluble extracts, represent a promising environment conducive to craniomaxillofacial bone repair.

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Glycosaminoglycan content of a mineralized collagen scaffold promotes mesenchymal stem cell secretion of factors to modulate angiogenesis and monocyte differentiation

Cited by 2 publications

References 80 publications

Decellularized blood vessel development: Current state-of-the-art and future directions

Decellularized blood vessel development: Current state-of-the-art and future directions

Soluble extracts from amnion and chorion membranes improve hMSC osteogenic response in a mineralized collagen scaffold

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