Glycosaminoglycans and glycoconjugates in the adult anuran integument (Lithobates catesbeianus)

Pelli, Adriana Alves; Cinelli, Leonardo Paes; Mourão, Paulo Antônio de Souza; Brito-Gitirana, Lycia de

doi:10.1016/j.micron.2010.03.001

Cited by 15 publications

(17 citation statements)

References 40 publications

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“…Although some studies of the anuran integument are available, such as those of bufonids (de Brito-Gitirana and Azevedo 2005, Almeida et al 2007, Felsemburgh et al 2009), ranids (Azevedo et al 2006, Pelli et al 2010, and leptodactylids (Goniakowska-Witalinska and Kubiczek 1998, Warburg et al 2000, Nosiet al 2002, Barbeau and Lillywhite 2005, Felsemburgh et al 2007, Gonçalves and de Brito-Gitirana 2008, Rigolo et al 2008), information about the integument structure of hylids is still insufficient.…”

Section: Discussionmentioning

confidence: 99%

“…In bufonids (de Brito-Gitirana and Azevedo 2005, Almeida et al 2007, Felsemburgh et al 2009), ranids (Azevedo et al 2006, Pelli et al 2010, and leptodactylids (Felsemburgh et al 2007, Gonçalves andde Brito-Gitirana 2008), apocrine granular glands occur scattered throughout the dorsal and ventral integument, exhibiting acidophilic granular secretion. In the integument of S. similis, the apocrine granular gland resembles those Ib type glands described for Engystomops pustulosus (Cope, 1864) (Delfino et al 2015).…”

Section: O Albicansmentioning

confidence: 99%

“…Moreover, the EK-layer was usually continuous in the dorsal integument, showing its typical basophilic and alcianophilic stainings, which were due to the glycoconjugate content. In R. icterica and L. catesbeianus, the EK-layer contained both dermatan sulfate and calcium, and occurred as scattered aggregates throughout the spongious dermis (Pelli et al , 2010. These mineral consists of calcium phosphate deposits (Katchburian et al 2001).…”

Section: O Albicansmentioning

confidence: 99%

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Comparative analysis of the integument of different tree frog species from Ololygon and Scinax genera (Anura: Hylidae)

Silva¹,

Silva-Soares²,

Brito-Gitirana³

2017

Zoologia

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ABSTRACT. The integuments of ten treefrog species of two genera from Scinaxnae -O. angrensis (Lutz, 1973), O. flavoguttata (Lutz & Lutz, 1939), O. humilis (Lutz & Lutz, 1954), O. perpusilla (Lutz & Lutz, 1939), O. v-signata (Lutz, 1968, Scinax hayii (Barbour, 1909), S. similis (Cochran, 1952), O. trapicheroi (Lutz & Lutz, 1954) and S. x-signatus (Spix, 1824) -were investigated using conventional and histochemical techniques of light microscopy, and polarized light microscopy. All integuments showed the basic structure of the anuran integument. Moreover, the secretory portions of exocrine glands, such as serous merocrine and apocrine glands, were found to be restricted to the spongious dermis. Lipid content occurred together with the heterogeneous secretory material of the glands with an apocrine secretion mechanism. In addition, clusters of these apocrine glands were present in the ventrolateral integument of some species. Melanophores were also visualized in all examined hylids. However, the occurrence of iridophores, detected through polarized light microscopy, varied according to the species. The Eberth-Katschenko layer occurred in the dorsal integument from both genera, but it was only present in the ventral integument of O. albicans, O. angrensis, O. flavoguttata, O. perpusilla and O. v-signata. Although the integument of all treefrogs showed the same basic structure, some characteristics were genus-specific; however, these features alone may not be used to distinguish both genera.

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Section: Discussionmentioning

confidence: 99%

Section: O Albicansmentioning

confidence: 99%

Section: O Albicansmentioning

confidence: 99%

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Comparative analysis of the integument of different tree frog species from Ololygon and Scinax genera (Anura: Hylidae)

Silva¹,

Silva-Soares²,

Brito-Gitirana³

2017

Zoologia

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“…The outer limit of epidermis is lined by keratinized cells and exhibits weak keratinization. In Lithobates catesbeianus (Shaw, 1802) (Pelli et al 2010), the epidermis was constituted by a squamous epithelium lightly keratinized in contrast to the keratinized epidermis described for Rhinella icterica (Spix, 1824) (former Bufo ictericus) (de Brito-Gitirana & Azevedo 2005). The keratinization grade is related to animal adaptation to terrestrial environment, because keratins protect epithelial cells from mechanical stress (Gu & Coulombe 2007;Pelli et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Investigation of dorsal/ventral skin and the parotoid region of Lyciasalamandra billae and Lyciasalamandra luschani basoglui (Urodela: Salamandridae)

2014

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In the present study, the parotoid region, dorsal and ventral integuments of Lyciasalamandra billae and Lyciasalamandra luschani basoglui were investigated in terms of localization of hyaluronic acid (HA) and histochemical characteristics. HA immunoreactivity was carried out using biotinylated hyaluronic acid binding protein (B-HABP) labelled with streptavidin-fluorescein isothiocyanate (FITC). HA was mainly localized in the stratum spongiosum of L. billae and L. luschani basoglui for water homeostasis and skin functionality. Light microscopic observations revealed that the dorsal and ventral integuments of L. billae and L. luschani basoglui exhibited basic morphological characteristics of other amphibians: the epidermis was composed of a stratified squamous epithelium and the dermis subdivided into stratum spongiosum and stratum compactum. Two different types of dermal glands (mucous and granular glands) were identified in the spongious dermis of the dorsal and ventral integuments whereas in the parotoid region, three different types of glands (mucous, granular and parotoid glands) were examined.

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“…Solid NaBH 4 (300 mg) was added slowly with stirring, and the solution was maintained at 50°C for 2 h. After the addition of several drops of acetic acid to destroy the excess of borohydride, the solution was dialyzed against distilled water and lyophilized to obtain the carboxyl-reduced PS (13,40). The extent of reduction of the carboxyl groups was estimated by the decrease in the carbazole reaction and subsequent formation of glucose (41,42).…”

Section: Methodsmentioning

confidence: 99%

Glucuronoxylomannan from Cryptococcus neoformans Down-regulates the Enzyme 6-Phosphofructo-1-kinase of Macrophages

Grechi¹,

Marinho-Carvalho²,

Zancan³

et al. 2011

Journal of Biological Chemistry

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The encapsulated yeast Cryptococcus neoformans is the causative agent of cryptococosis, an opportunistic life-threatening infection. C. neoformans is coated by a polysaccharide capsule mainly composed of glucuronoxylomannan (GXM). GXM is considered a key virulence factor of this pathogen. The present work aimed at evaluating the effects of GXM on the key glycolytic enzyme, 6-phosphofructo-1-kinase (PFK). GXM inhibited PFK activity in cultured murine macrophages in both dose-and time-dependent manners, which occurred in parallel to cell viability decrease. The polysaccharide also inhibited purified PFK, promoting a decrease on the enzyme affinity for its substrates. In macrophages GXM and PFK partially co-localized, suggesting that internalized polysaccharide directly may interact with this enzyme. The mechanism of PFK inhibition involved dissociation of tetramers into weakly active dimers, as revealed by fluorescence spectroscopy. Allosteric modulators of the enzyme able to stabilize its tetrameric conformation attenuated the inhibition promoted by GXM. Altogether, our results suggest that the mechanism of GXM-induced cell death involves the inhibition of the glycolytic flux.

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Glycosaminoglycans and glycoconjugates in the adult anuran integument (Lithobates catesbeianus)

Cited by 15 publications

References 40 publications

Comparative analysis of the integument of different tree frog species from Ololygon and Scinax genera (Anura: Hylidae)

Comparative analysis of the integument of different tree frog species from Ololygon and Scinax genera (Anura: Hylidae)

Investigation of dorsal/ventral skin and the parotoid region of Lyciasalamandra billae and Lyciasalamandra luschani basoglui (Urodela: Salamandridae)

Glucuronoxylomannan from Cryptococcus neoformans Down-regulates the Enzyme 6-Phosphofructo-1-kinase of Macrophages

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