Glycosaminoglycans from fresh water fish processing discard - Isolation, structural characterization, and osteogenic activity

Gavva, Chandra; Patel, Kalpana; Kudre, Tanaji G.; Sharan, Kunal; Chilkunda, D Nandini

doi:10.1016/j.ijbiomac.2019.12.189

Cited by 18 publications

(6 citation statements)

References 62 publications

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“…Thus, the spectral assignments of 1 H NMR confirmed the presence of CS type GAG in fish gills of T. albacares as it encloses peaks which might be consigned to CS type GAG and these conclusions also agree to the published results [39,40]. Similar work asserted the occurrence of CS type GAG from the processing discards of Labeorohita and Piaractus brachypomus [41] and from chain cap of articular cartilage [42] . The physical and chemical analysis revealed that the GAG isolated from the gills of T. albacares are mainly CS type.…”

Section: Discussionsupporting

confidence: 89%

Fish gills of Thunnus albacares: A novel source of chondroitin sulphate glycosaminoglycans

Thomas¹,

Kp²,

Mathew³

2021

Int. J. Fish. Aquat. Stud.

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Fish gills of Thunnus albacares have been chosen as a unique source of glycosaminoglycans (GAGs) in this study. Chemical and physical characterization were carried out by ATR -FTIR spectroscopy, DEAE -Cellulose anion exchange chromatography, SAX -HPLC, and NMR techniques to determine the nature of GAG isolated. The results showed that the isolated GAGs (8.5mg/g yield) were mostly Chondroitin sulphate (CS) type. SAX HPLC chromatogram of isolated GAG and CS A standard showed comparable peaks A at 4.9, B at 7.8 and C at 9.99 min. FTIR spectra, IR (KBr) υ max in cm -1 presented characteristic peaks of -CONH vibration of amide group, coupling of C-O stretching vibration, S=O stretching vibrations and -C-O-S molecules confirmed the presence of CS type GAG in T. albacaresgills. 1 H NMR spectra in D2Oalso revealed the presence of CS moiety. All these results substantiate T. albacares gills as an excellent source of CS type GAG.

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Section: Discussionsupporting

confidence: 89%

Fish gills of Thunnus albacares: A novel source of chondroitin sulphate glycosaminoglycans

Thomas¹,

Kp²,

Mathew³

2021

Int. J. Fish. Aquat. Stud.

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“…In order to ensure that fish wastes are utilized to their best capacity, as shown in Table 2 , CS was extracted from a variety of fish body parts [ 17 , 19 , 23 , 30 , 31 , 32 , 33 , 34 , 35 ]. These body parts are cartilage, the head, the eyes, the fins, and the fish’s skin.…”

Section: Chondroitin Sulphatementioning

confidence: 99%

“…Alternative and safe ways to manufacture CS are now being researched [ 8 ]. Fish (both marine and freshwater) have been investigated as alternate halal sources of GAGs compared to terrestrial GAGs, including porcine [ 8 , 16 , 17 ]. Therefore, the CS production experiment has increased demand for its use as a versatile chemical in recent years.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the CS production experiment has increased demand for its use as a versatile chemical in recent years. Fishbones, heads, gills, viscera, fins, eyeballs, and scales of marine and freshwater fish species are used to extract CS [ 17 , 18 , 19 ]. It has a different concentration and composition depending on where it comes from.…”

Section: Introductionmentioning

confidence: 99%

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A Concise Review of Extraction and Characterization of Chondroitin Sulphate from Fish and Fish Wastes for Pharmacological Application

Urbi

Azmi

Ming

et al. 2022

CIMB

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Chondroitin sulphate (CS) is one of the most predominant glycosaminoglycans (GAGs) available in the extracellular matrix of tissues. It has many health benefits, including relief from osteoarthritis, antiviral properties, tissue engineering applications, and use in skin care, which have increased its commercial demand in recent years. The quest for CS sources exponentially increased due to several shortcomings of porcine, bovine, and other animal sources. Fish and fish wastes (i.e., fins, scales, skeleton, bone, and cartilage) are suitable sources of CS as they are low cost, easy to handle, and readily available. However, the lack of a standard isolation and characterization technique makes CS production challenging, particularly concerning the yield of pure GAGs. Many studies imply that enzyme-based extraction is more effective than chemical extraction. Critical evaluation of the existing extraction, isolation, and characterization techniques is crucial for establishing an optimized protocol of CS production from fish sources. The current techniques depend on tissue hydrolysis, protein removal, and purification. Therefore, this study critically evaluated and discussed the extraction, isolation, and characterization methods of CS from fish or fish wastes. Biosynthesis and pharmacological applications of CS were also critically reviewed and discussed. Our assessment suggests that CS could be a potential drug candidate; however, clinical studies should be conducted to warrant its effectiveness.

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“…Following previously published protocols, the mouse primary calvarial osteoblast cell culture was carried out (80). Briefly, 6-8 calvaria were harvested from newborn pups.…”

Section: Mcobs Culturementioning

confidence: 99%

Maternal high-cholesterol diet negatively programs offspring bone development and downregulates hedgehog signaling in osteoblasts

Mangu¹,

Patel²,

Sukhdeo³

et al. 2022

Journal of Biological Chemistry

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Glycosaminoglycans from fresh water fish processing discard - Isolation, structural characterization, and osteogenic activity

Cited by 18 publications

References 62 publications

Fish gills of Thunnus albacares: A novel source of chondroitin sulphate glycosaminoglycans

Fish gills of Thunnus albacares: A novel source of chondroitin sulphate glycosaminoglycans

A Concise Review of Extraction and Characterization of Chondroitin Sulphate from Fish and Fish Wastes for Pharmacological Application

Maternal high-cholesterol diet negatively programs offspring bone development and downregulates hedgehog signaling in osteoblasts

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