Glycosaminoglycans in horses with osteoarthritis

Alwan, W. H.; Carter, S. D.; Bennett, David; Edwards, Glenn S.

doi:10.1111/j.2042-3306.1991.tb02712.x

Cited by 50 publications

(46 citation statements)

References 13 publications

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“…Cartilage matrix degeneration causes the release of GAG into synovial fluids. Thus, changes of GAG levels in cartilage or synovial fluids reflect the extent of catabolic activity in the cartilage matrix (Alwan et al, 1991;Poole et al, 1994). In this study, bFGF alone did not reduce GAG content in synovial fluid as expected while the other factors took effects.…”

Section: Discussionmentioning

confidence: 51%

Effects of adenovirus-mediated bFGF, IL-1Ra and IGF-1 gene transfer on human osteoarthritic chondrocytes and osteoarthritis in rabbits

et al. 2010

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Abbreviations: ACLT, anterior cruciate ligament transaction; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; bFGF, basic fibroblast growth factor; Ct, threshold cycle; DMMB, dimethylmethylene blue; ECM, extracellular matrix; GAG, glycosaminoglycan; IGF-1, insulin-like growth factor-1; IL-1Ra, interleukin-1 receptor antagonist protein; MMP, matrix metalloproteinase; OA, osteoarthritis; TIMP, tissue inhibitor of metalloproteinase AbstractThe study investigated the effects of adenovirus-mediated gene transfection of basic fibroblast growth factor (bFGF), bFGF combined with interleukin-1 receptor antagonist protein (IL-Ra) and/or insulin-like growth factor-1 (IGF-1) both in human osteoarthritis (OA) chondrocytes and rabbits OA model. Human OA chondrocytes were delivered by adenovirus-mediated bFGF, IL-Ra and IGF-1 vectors, respectively. Chondrocyte proliferation, glycosaminoglycan (GAG) content, expression of type II collagen, ADAMTS-5, MMP-13, MMP-3 and TIMP-1 were determined. Rabbit OA model was induced by anterior cruciate ligament transaction (ACLT) in knees. Adenoviral vectors encoding human bFGF, IL-Ra and IGF-1 were injected intraarticularly into the knee joints after ACLT. The effects of adenovirus-mediated gene transfection on rabbit OA were evaluated. In vitro, the transfected genes were expressed in cell supernatant of human OA chondrocytes. AdbFGF group significantly promoted chondrocyte proliferation, and increased GAG and type II collagen synthesis than in the OA group. As two or three genes were transfected in different combinations, there was significant enhancement on the GAG content, type II collagen synthesis, and TIMP-1 levels, while ADAMTS-5, MMP-13, and MMP-3 levels were reduced. In vivo, the transfected genes were expressed in synovial fluid of rabbits. Intraarticular delivery of bFGF enhanced the expression of type II collagen in cartilage and decreased cartilage Mankin score compared with the OA control group (P = 0.047; P ＜ 0.01, respectively). Multiple-gene transfection in different combinations showed better results than bFGF transfection alone. This study suggests that bFGF gene transfection is effective in treating experimental OA. Multiple gene transfection has better biologic effects on OA.

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Section: Discussionmentioning

confidence: 51%

Effects of adenovirus-mediated bFGF, IL-1Ra and IGF-1 gene transfer on human osteoarthritic chondrocytes and osteoarthritis in rabbits

et al. 2010

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“…1993), Glycosaminoglycans (GAGs) (keratan sulfate and chondroitin sulfate) are part of the proteoglycan molecule in the articular cartilage matrix. Increased levels of these substances have been found in the synovial fluid of horses with naturally occurring OA (Alwan et al 1990(Alwan et al , 1991bLittle eI al. 1990).…”

Section: Diagnosismentioning

confidence: 99%

Equine osteoarthritis – A review of pathogenesis, diagnosis and treatment

Jansson¹

1996

PHK

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“…Total glycosaminoglycan concentration was evaluated by Farndale' method, modified by Alwan [13,14] . Concentrations of diclofenac in synovial fluid and plasma samples were measured by an HPLC method with UV detection described by Krishna & Surja Kumar (1991).…”

Section: Measurements and Analytical Methodsmentioning

confidence: 99%

Inefficacy of Topical Diclofenac in Arthritic Horses

Villarino¹,

Vispo²,

Marcos³

et al. 2006

American J. of Animal and Veterinary Sciences

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Abstract:The objective of the present study was test the anti-inflammatory efficacy of diclofenac dietilamine, applied epicutaneously at a dose rate of 0.44 mg kg 1 , in a model of acute arthritis in horses. Four clinical end-points, as well as two biochemical markers, were used as surrogate markers of the required clinical response (analgesia, anti-inflammatory). Low diclofenac concentrations were measured in blood (C max 0.04±0.03 ug mL 1 ) and synovial fluid (C max 0.08 ±0.08 ug mL 1 ) from the first to the last sampling time. The statistical comparison of the clinical end-points and biochemical markers between placebo and dicilofenac treated group indicated a lack of pharmacological effect of this compound after epicutaneous administration.

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Glycosaminoglycans in horses with osteoarthritis

Cited by 50 publications

References 13 publications

Effects of adenovirus-mediated bFGF, IL-1Ra and IGF-1 gene transfer on human osteoarthritic chondrocytes and osteoarthritis in rabbits

Effects of adenovirus-mediated bFGF, IL-1Ra and IGF-1 gene transfer on human osteoarthritic chondrocytes and osteoarthritis in rabbits

Equine osteoarthritis – A review of pathogenesis, diagnosis and treatment

Inefficacy of Topical Diclofenac in Arthritic Horses

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