Glycosaminoglycans of the Vertebral Body Growth Plate in Patients with Idiopathic Scoliosis

Abstract

The concentrations of keratan sulfates and unmodified keratan sulfates increased in the vertebral body growth plate in patients with idiopathic scoliosis. Sulfation and acetylation of total glycosaminoglycans decreased by 50 and 30%, respectively. These changes reflect the decrease in biological activity of molecules that modulate function of the growth plate.

Glycosaminoglycans of the trabecular meshwork of the eye in primary juvenile glaucoma

“…Incubation was carried out for 72 h at 4 o C, after which the solution was dialyzed against 50 mM Na acetate buffer (pH 5.8) for 18 h at 4 o C. Proteins were precipitated by adding 100% trichloroacetic acid to a final concentration of 5% to samples, centrifuged, and the solution was dialyzed against 50 mM Na acetate buffer (pH 5.8) for 18 h at 4 o C. PG was precipitated with 3 volumes of 96% ethanol with 4% potassium acetate with subsequent centrifugation; the precipitate was dissolved in deionized water [2].…”

Adolescent idiopathic scoliosis: evidence for intrinsic factors driving aetiology and progression

“…However, histopathological studies have provided evidence for the disorganisation of columns of chondrocytes in the convex zone of the growth plate from a patient with AIS [14]. Furthermore, several studies have provided evidence for intervertebral disc matrix degeneration in AIS, with reduced proteoglycan content [50], decreased sulphation and acetylation of proteoglycans within the cartilaginous end plate and nucleus pulposus [52]. In addition, in the annulus fibrosus of AIS patients, vacuolation [41] and abnormal localisation of collagen fibres have been reported [7].…”

Morphofunctional Laws Regulating Chondrogenesis in Vertebral and Iliac Bone Growth Plates

“…Биохимическими методами исследовали качественное и количественное содержание гликозаминогликанов (ГАГ) в тканях ПР тел позвонков и подвздошного хряща. Для выделения ГАГ ткань хряща обрабатывали раствором папаина по общепринятой методике [2]. В растворе аналитическими методами определяли содержание химических компонентов ГАГ -сульфатированных ГАГ (СГАГ), гексуроновых кислот и гексоз.…”