Glycosyl hydrolases from <i>Bifidobacterium adolescentis </i>DSM20083. An overview

Broek, L.A.M. van den; Hinz, S.W.A.; Beldman, G.; Doeswijk-Voragen, Chantal H. L.; Vincken, Jean‐Paul; Voragen, A.G.J.

doi:10.1051/lait:2005004

Cited by 11 publications

(11 citation statements)

References 36 publications

(40 reference statements)

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“…Similar results were found for the heterologously produced enzyme [123,125,126]. In contrast to the preferred formation of 1 6 linkages during the transfer reaction, Leder et al [122] showed that 1 3 linkages are hydrolyzed at a higher rate than 1 6 linkages.…”

Section: A-galacto-oligosaccharides and Galactomannansupporting

confidence: 62%

“…In most cases, the for-mer is more predominant than the latter. For Bifidobacterium a-galactosidase we have found that, under the right conditions 69% of the cleaved galactose units are used in the transglycosylation reaction with melibiose as substrate [126]. Usually, the balance between hydrolysis and transglycosylation is much less favorable.…”

Section: Strategies For Efficient Prebiotic Oligosaccharide Productionmentioning

confidence: 99%

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Bifidobacterium carbohydrases‐their role in breakdown and synthesis of (potential) prebiotics

Broek

Hinz

Beldman

et al. 2008

Molecular Nutrition Food Res

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145

109

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There is an increasing interest to positively influence the human intestinal microbiota through the diet by the use of prebiotics and/or probiotics. It is anticipated that this will balance the microbial composition in the gastrointestinal tract in favor of health promoting genera such as Bifidobacterium and Lactobacillus. Carbohydrates like non-digestible oligosaccharides are potential prebiotics. To understand how these bacteria can grow on these carbon sources, knowledge of the carbohydrate-modifying enzymes is needed. Little is known about the carbohydrate-modifying enzymes of bifidobacteria. The genome sequence of Bifidobacterium adolescentis and Bifidobacterium longum biotype longum has been completed and it was observed that for B. longum biotype longum more than 8% of the annotated genes were involved in carbohydrate metabolism. In addition more sequence data of individual carbohydrases from other Bifidobacterium spp. became available. Besides the degradation of (potential) prebiotics by bifidobacterial glycoside hydrolases, we will focus in this review on the possibilities to produce new classes of non-digestible oligosaccharides by showing the presence and (transglycosylation) activity of the most important carbohydrate modifying enzymes in bifidobacteria. Approaches to use and improve carbohydrate-modifying enzymes in prebiotic design will be discussed.

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Section: A-galacto-oligosaccharides and Galactomannansupporting

confidence: 62%

Section: Strategies For Efficient Prebiotic Oligosaccharide Productionmentioning

confidence: 99%

Bifidobacterium carbohydrases‐their role in breakdown and synthesis of (potential) prebiotics

Broek

Hinz

Beldman

et al. 2008

Molecular Nutrition Food Res

Self Cite

145

109

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show abstract

“…These enzymes are essential for bifidobacteria to be able to grow in milk or milk-components like lactose and lactose-derived transgalacto-oligosaccharides (TOS). Arabinofuranosyl-containing oligosaccharides derived from plant cell-wall constituents, such as arabinan and arabinoxylan, can be fermented by bifidobacteria through arabinoxylan- and arabinofurano-hydrolases [ 93 ]. However, the rate of degradation of these compounds is rather low and it has been assumed that other bacteria (e.g.…”

Section: Identification Of Existing and Novel Biochemical Routesmentioning

confidence: 99%

Genome Analysis of Food Grade Lactic Acid-Producing Bacteria: From Basics to Applications

Mayo

Sinderen²,

Ventura³

2008

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Whole-genome sequencing has revolutionized and accelerated scientific research that aims to study the genetics, biochemistry and molecular biology of bacteria. Lactic acid-producing bacteria, which include lactic acid bacteria (LAB) and bifidobacteria, are typically Gram-positive, catalase-negative organisms, which occupy a wide range of natural plant-and animal-associated environments. LAB species are frequently involved in the transformation of perishable raw materials into more stable, pleasant, palatable and safe fermented food products. LAB and bifidobacteria are also found among the resident microbiota of the gastrointestinal and/or genitourinary tracts of vertebrates, where they are believed to exert health-promoting effects. At present, the genomes of more than 20 LAB and bifidobacterial species have been completely sequenced. Their genome content reflects its specific metabolism, physiology, biosynthetic capabilities, and adaptability to varying conditions and environments. The typical LAB/bifidobacterial genome is relatively small (from 1.7 to 3.3 Mb) and thus harbors a limited assortment of genes (from around 1,600 to over 3,000). These small genomes code for a broad array of transporters for efficient carbon and nitrogen assimilation from the nutritionally-rich niches they usually inhabit, and specify a rather limited range of biosynthetic and degrading capabilities. The variation in the number of genes suggests that the genome evolution of each of these bacterial groups involved the processes of extensive gene loss from their particular ancestor, diversification of certain common biological activities through gene duplication, and acquisition of key functions via horizontal gene transfer. The availability of genome sequences is expected to revolutionize the exploitation of the metabolic potential of LAB and bifidobacteria, improving their use in bioprocessing and their utilization in biotechnological and health-related applications.

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“…There are relatively few publications that review characterized carbohydrate hydrolases of Bifidobacterium sp. [141,142,145]. Therefore, this review will focus on the currently available knowledge on bifidobacterial carbohydrate metabolism, covering the utilization of monosaccharides, disaccharides, oligosaccharides and polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

Carbohydrate metabolism in Bifidobacteria

2011

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Members of the genus Bifidobacterium can be found as components of the gastrointestinal microbiota, and are believed to play an important role in maintaining and promoting human health by eliciting a number of beneficial properties. Bifidobacteria can utilize a diverse range of dietary carbohydrates that escape degradation in the upper parts of the intestine, many of which are plantderived oligo-and polysaccharides. The gene content of a bifidobacterial genome reflects this apparent metabolic adaptation to a complex carbohydrate-rich gastrointestinal tract environment as it encodes a large number of predicted carbohydrate-modifying enzymes. Different bifidobacterial strains may possess different carbohydrate utilizing abilities, as established by a number of studies reviewed here. Carbohydrate-degrading activities described for bifidobacteria and their relevance to the deliberate enhancement of number and/or activity of bifidobacteria in the gut are also discussed in this review.

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Glycosyl hydrolases from Bifidobacterium adolescentis DSM20083. An overview

Cited by 11 publications

References 36 publications

Bifidobacterium carbohydrases‐their role in breakdown and synthesis of (potential) prebiotics

Bifidobacterium carbohydrases‐their role in breakdown and synthesis of (potential) prebiotics

Genome Analysis of Food Grade Lactic Acid-Producing Bacteria: From Basics to Applications

Carbohydrate metabolism in Bifidobacteria

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