Glycosylated Cathepsin V Serves as a Prognostic Marker in Lung Cancer

Qi, Zhang; Ruan, Wenquan; Qiu, Yeqing; Yao, Wei; Liao, Yifeng

doi:10.3389/fonc.2022.876245

Cited by 15 publications

(11 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Researches indicate that abnormal proteolytic activity may trigger the manifestation of diseased conditions [18]. CTSV had been proved to be a proteolytic enzyme that is overexpressed in metastasis lung cancer patients and predicted poorer outcome [19], here we speculated that CTSV interact with certain substrates and remodel ECM environment. To this end, we performed protein mass spectrometry of both NCI-H1975 and A549 cells and identi ed that CTSV interacts with bronectin, E-cadherin and Ncadherin, which are essential molecules for metastasis (Supplementary information, Figure S2a).…”

Section: Ctsv Mediated Fragmentation Of Bronectin E-cadherin and N-ca...mentioning

confidence: 83%

Cathepsin V plays a crucial role in the metastasis and growth of lung cancer

Wang

Xiao

Shi

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Cathepsin V (CTSV) is a cysteine cathepsin protease located in lysosome and plays crucial role in extracellular matrix degradation. It is reported that CTSVis correlated with poor prognosis with multiple cancers, but the underlying mechanism remains unclear. In this study, we aimed to clarify the molecular mechanism of how CTSV functions in lung cancer. Here, we identified that CTSV is upregulated in high metastatic property lung cancer cells. Depletion of CTSV inhibited the metastasis and growth of lung cancer. Mechanistic studies showed that CTSV functions by cleaving fibronectin, E-cadherin and N-cadherin and remodeling the extracellular matrix (ECM) of lung cancer. Our data showed that CTSV is an essential component of lysosome, reduction of CTSV increased pH value of lysosome and led to lysosome dysfunction. Together, our findings reveal a molecular mechanism of how CTSV drive metastasis and growth of lung cancer, suggesting that CTSV-targeting approach is a promising strategy for lung cancer.

show abstract

Section: Ctsv Mediated Fragmentation Of Bronectin E-cadherin and N-ca...mentioning

confidence: 83%

Cathepsin V plays a crucial role in the metastasis and growth of lung cancer

Wang

Xiao

Shi

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Despite its involvement in various cancers, the specific role of CTSV in cSCC remains unclear. Notably, high CTSV expression has been linked to unfavourable outcomes in breast ductal carcinoma, 33 promotes proliferation through the NF‐κB pathway in bladder cancer, 34 and drives lung cancer metastasis 35 . Conversely, CTSV is reported to be decreased in melanoma samples 36 .…”

Section: Discussionmentioning

confidence: 99%

“…Notably, high CTSV expression has been linked to unfavourable outcomes in breast ductal carcinoma, 33 promotes proliferation through the NF-κB pathway in bladder cancer, 34 and drives lung cancer metastasis. 35 Conversely, CTSV is reported to be decreased in melanoma samples. 36 To fully comprehend the role of CTSV in cSCC development, further investigation is warranted.…”

Section: Proteomic Analysis Identifies Non-invasive Protein Signature...mentioning

confidence: 99%

Non‐invasive diagnosis of early cutaneous squamous cell carcinoma

Azimi,

Jabbour,

Patrick

et al. 2023

Experimental Dermatology

View full text Add to dashboard Cite

Early cutaneous squamous cell carcinoma (cSCC) can be challenging to diagnose using clinical criteria as it could present similar to actinic keratosis (AK) or Bowen's disease (BD), precursors of cSCC. Currently, histopathological assessment of an invasive biopsy is the gold standard for diagnosis. A non‐invasive diagnostic approach would reduce patient and health system burden. Therefore, this study used non‐invasive sampling by tape‐stripping coupled with data‐independent acquisition mass spectrometry (DIA‐MS) proteomics to profile the proteome of histopathologically diagnosed AK, BD and cSCC, as well as matched normal samples. Proteomic data were analysed to identify proteins and biological functions that are significantly different between lesions. Additionally, a support vector machine (SVM) machine learning algorithm was used to assess the usefulness of proteomic data for the early diagnosis of cSCC. A total of 696 proteins were identified across the samples studied. A machine learning model constructed using the proteomic data classified premalignant (AK + BD) and malignant (cSCC) lesions at 77.5% accuracy. Differential abundance analysis identified 144 and 21 protein groups that were significantly changed in the cSCC, and BD samples compared to the normal skin, respectively (adj. p < 0.05). Changes in pivotal carcinogenic pathways such as LXR/RXR activation, production of reactive oxygen species, and Hippo signalling were observed that may explain the progression of cSCC from premalignant lesions. In summary, this study demonstrates that DIA‐MS analysis of tape‐stripped samples can identify non‐invasive protein biomarkers with the potential to be developed into a complementary diagnostic tool for early cSCC.

show abstract

“…The advent of high-resolution MS has led to the discovery of novel, specifically expressed protein biomarkers that may be involved in the pathophysiology of diseases that are difficult to identify by immunohistochemistry and histologic morphology alone [ 66 ]. The importance of MS in identifying the target proteins involved in virtually any disease process makes it an invaluable discovery tool since MS can provide a catalog of novel target proteins implicated in health and disease.…”

Section: Discussionmentioning

confidence: 99%

Identification of Target Proteins Involved in Cochlear Hair Cell Progenitor Cytotoxicity following Gentamicin Exposure

Davies

Mittal

et al. 2022

JCM

View full text Add to dashboard Cite

Given the non-labile, terminal differentiation of inner-ear sensory cells, preserving their function is critical since sensory cell damage results in irreversible hearing loss. Gentamicin-induced cytotoxicity is one of the major causes of sensory cell damage and consequent sensorineural hearing loss. However, the precise molecular mechanisms and target proteins involved in ototoxicity are still unknown. The objective of the present study was to identify target proteins involved in gentamicin-induced cytotoxicity to better characterize the molecular pathways involved in sensory cell damage following ototoxic drug administration using House Ear Institute-Organ of Corti 1 (HEI-OC1) cells and high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). We identified several unique proteins involved in gentamicin-induced cytotoxicity, expression of which were further confirmed using confocal microscopy. Further investigation of these pathways can inform the design and discovery of novel treatment modalities to prevent sensory cell damage and preserve their function.

show abstract

Glycosylated Cathepsin V Serves as a Prognostic Marker in Lung Cancer

Cited by 15 publications

References 42 publications

Cathepsin V plays a crucial role in the metastasis and growth of lung cancer

Cathepsin V plays a crucial role in the metastasis and growth of lung cancer

Non‐invasive diagnosis of early cutaneous squamous cell carcinoma

Identification of Target Proteins Involved in Cochlear Hair Cell Progenitor Cytotoxicity following Gentamicin Exposure

Contact Info

Product

Resources

About