Glycosylation alterations of cells in late phase apoptosis from colon carcinomas

Abstract

Comparisons of carbohydrate profiles between control and apoptotic colon carcinoma cells were performed by flow cytometry using a set of lectins and anti-carbohydrate antibodies. The six cell lines analyzed presented distinct carbohydrate profiles before induction of apoptosis. PHA-L and MAA binding decreased after induction of apoptosis by UV-treatment. In contrast an increase of PNA binding was observed after induction of apoptosis, except on SW-48 cells for which a decrease occurred. A decrease of SNA binding was observed after induction of apoptosis from strongly positive control cell lines, whereas it increased on weakly positive ones. All the blood group related antigens A, H, Lewis a, Lewis x, Lewis b, and Lewis y, had their expression strongly diminished on apoptotic cells. These changes occurred irrespective of the mode of apoptosis induction since similar results were obtained after UV, TNFalpha, or anti-Fas treatment. Fucosyltransferases activities were also decreased after apoptosis induction, except for alpha1,3fucosyltransferase in anti-Fas treated HT-29 cells, where it was strongly augmented. This could be attributed to the IFNgamma preteatment required to induce Fas expression on these cells. Fucosidase activity decreased after induction of apoptosis suggesting that it was not responsible for the loss of fucosylated structures. In the rat PRO cell line, H blood group antigens are mainly carried by a high molecular weight variant of CD44. It could be shown that the loss of H antigen after induction of apoptosis correlated with a loss of the carrier glycoprotein.

Decrease of sialic acid residues as aneat-mesignal on the surface of apoptotic lymphocytes

“…Changes in the glycomic profile of apoptotic cells have previously been studied by various groups, who have analyzed cells of different origin (Azuma et al, 2002;Hart et al, 2000;Rapoport and Pendu, 1999). In these previous studies, both an increase and a decrease of membrane-bound Sias have been reported, depending on the cell type under investigation.…”

Increased Expression of Lewis X and Y Antigens on the Cell Surface and FUT 4 mRNA during Granzyme B-Induced Jurkat Cell Apoptosis

“…In contrast, IFNg alone up-regulates FUT4 mRNA in HT-29 cells, and expression of all blood group-related antigens, including Lewis X and Y antigens, are suppressed in late apoptosis. 29) IFNg and some chemotherapeutic drugs also up-regulate Lewis Y antigens on HT-29 cells.…”

Structural Basis of the Catalytic Reaction Mechanism of Novel 1,2-α-L-Fucosidase from Bifidobacterium bifidum

“…Fucosecontaining carbohydrates play important roles in diverse cellular and physiologic processes, including inflammatory responses (16 -18) and antigenic determination (19). Furthermore, altered expression of ␣-L-fucosidases and corresponding changes in fucosylation levels are seen in many carcinomas (20,21). Accordingly, the presence of human ␣-L-fucosidase in the serum can be used as an early detection marker for several carcinomas (22)(23)(24).…”