Glycosylation of ascites-derived exosomal CD133: a potential prognostic biomarker in patients with advanced pancreatic cancer

Sakaue, Takahiko; Koga, Hironori; Iwamoto, Hideki; Nakamura, Tõru; Ikezono, Yu; Abe, Mitsuhiko; Wada, Fumitaka; Masuda, Atsutaka; Tanaka, Toshimitsu; Fukahori, Masaru; Mihara, Yuutarou; Naitou, Yoshiki; Okabe, Yoshinobu; Kakuma, Tatsuyuki; Ohta, Keisuke; Nakamura, Kei‐ichiro; Torimura, Takuji

doi:10.1007/s00795-019-00218-5

Cited by 44 publications

(32 citation statements)

References 26 publications

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“…CD133 is encoded by PROM1 gene composed of 8 N-linked glycosylation sites (93), and the glycosylation plays important roles in maintaining CSC properties (94,95). In fact, glycosylation of CD133 has been suggested as a secondary indicator of CSCs (96)(97)(98). In particular, sialylation of CD133 N-glycan terminal via a2,3-site was found in the CSC of glioma (54), which was augmented in hypoxic conditions, correlating with the migration and survival of brain CSCs (99).…”

Section: Glycosylation Of Csc Markersmentioning

confidence: 99%

Abnormal Glycosylation of Cancer Stem Cells and Targeting Strategies

Khan

Cabral

2021

Front. Oncol.

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Cancer stem cell (CSCs) are deemed as one of the main reasons of tumor relapse due to their resistance to standard therapies. Numerous intracellular signaling pathways along with extracellular features are crucial in regulating CSCs properties, such as heterogeneity, plasticity and differentiation. Aberrant glycosylation of these cellular signaling pathways and markers of CSCs have been directly correlated with maintaining survival, self-renewal and extravasation properties. In this review, we highlight the importance of glycosylation in promoting stemness character of CSCs, and present strategies for targeting abnormal glycosylation to eliminate the resistant CSC population.

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Section: Glycosylation Of Csc Markersmentioning

confidence: 99%

Abnormal Glycosylation of Cancer Stem Cells and Targeting Strategies

Khan

Cabral

2021

Front. Oncol.

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“…translate to better prognosis. 6 As mentioned above, urine is undoubtedly an ideal source of exosomes for assessing exosomal glycosylation, as urine is easily accessible. Such analysis can be used for detecting various kinds of dysfunction in diverse organs and for diagnoses.…”

Section: F I G U R Ementioning

confidence: 99%

“…Pancreatic cancer‐related exosomes also are found in ascites. Sakaue et al discovered that exosomes derived from pancreatic patients express a glycoprotein (CD133) biomarker for pancreatic cancer 6 . Importantly, they demonstrated that the degree CD133 glycosylation is associated with patient prognosis, such that higher degrees of glycosylation (eg, sialic acids, GlcNAc, mannose, etc.)…”

Section: Glycosylation Of Exosomes Can Serve As Biomarkers Of Diseasementioning

confidence: 99%

“…Importantly, they demonstrated that the degree CD133 glycosylation is associated with patient prognosis, such that higher degrees of glycosylation (eg, sialic acids, GlcNAc, mannose, etc.) translate to better prognosis 6 …”

Section: Glycosylation Of Exosomes Can Serve As Biomarkers Of Diseasementioning

confidence: 99%

“…Under the electron microscope, it is apparent that exosomes have a disc-shaped 3D structure, rather than a spherical structure one might imagine. Exosomes can be found in various body fluids, for example, blood, urine, ascites, and saliva, [3][4][5][6][7] or in conditioned culture medium of in vitro of cultured cell lines. 5,8 They contain nucleic acids, lipids, and proteins, all of which may serve as important exosome-specific biomarkers or specific glycanrelated disease biomarkers.…”

mentioning

confidence: 99%

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The “sugar‐coated bullets” of cancer: Tumor‐derived exosome surface glycosylation from basic knowledge to applications

Lin

Zhou

Tan

2020

Clinical & Translational Med

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Scientific interest in exosomes has exploded in recent decades. In 1990 only three articles were published on exosomes, while over 1,700 have already been published halfway into 2020. 1 While researchers have shown much interest in exosomes since being discovered in 1981, an appreciation of the potential role of glycans in exosome structure and function has emerged only recently. Glycosylation is one of the most common post-translational modification, which functions in many physiological and pathological aspects of cellular function. Many components of exosomes are heavily glycosylated including proteins, lipids, among others. Thus, glycosylation undoubtedly has a great impact on exosome biosynthesis and function. Despite the importance of glycosylation in exosomes and the recent recognition of them as biomarkers for not only malignancies but also other system dysfunction and disease, the characterization of exosome glycans remains understudied. In this review, we discuss glycosylation patterns of exosomes derived from various tissues, their biological features, and potential for various clinical applications. We highlight state-of-the-art knowledge about the fine structure of exosomes, which will allow researchers to reconstruct them by surface modification. These efforts will likely lead to novel disease-related biomarker discovery, purification tagging, and targeted drug transfer for clinical applications in the future.

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Bioaffinity Recognition of Extracellular Vesicle Glycosylations

Islam,

Leivo

2024

Biology of Extracellular Matrix

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Glycosylation of ascites-derived exosomal CD133: a potential prognostic biomarker in patients with advanced pancreatic cancer

Cited by 44 publications

References 26 publications

Abnormal Glycosylation of Cancer Stem Cells and Targeting Strategies

Abnormal Glycosylation of Cancer Stem Cells and Targeting Strategies

The “sugar‐coated bullets” of cancer: Tumor‐derived exosome surface glycosylation from basic knowledge to applications

Bioaffinity Recognition of Extracellular Vesicle Glycosylations

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