Glycosylation of hemocyanin in Litopenaeus vannamei is an antibacterial response feature

Zhang, Zehui; Wang, Fan; Chen, Chuandao; Zheng, Zhou; Aweya, Jude Juventus; Zhang, Yueling

doi:10.1016/j.imlet.2017.10.008

Cited by 36 publications

(23 citation statements)

References 34 publications

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“…B). Furthermore, our previous study showed that the agglutinative activity of hemocyanin toward Vibrio fluvialis and Vibrio alginolyticus was reduced by about fourfold and eightfold, respectively, after deglycosylation, suggesting that O‐glycosylation of L. vannamei hemocyanin closely relate to its immune function .…”

Section: Discussionmentioning

confidence: 97%

“…Purification of hemocyanin was performed by affinity chromatography as previously described with some modifications . Briefly, L. vannamei sera (2 mL) were loaded onto an affinity chromatography column with a ligand of rabbit anti‐shrimp small subunit hemocyanin antibody, followed by washing the column with PBS (0.01 m , pH 7.4) until the absorbance at 280 nm reached baseline.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, our previous studies revealed that the diversity in the glycosylation of L. vannamei hemocyanin was related to its agglutinative activity against bacteria, as hemocyanin with five different glycans had different agglutinative activity against E. coli, S. aureus, and erythrocytes [28]. Moreover, the agglutinative activity of hemocyanin was significantly reduced after a reduction in the glycan level [28,29]. However, prior to this study, little was known about the glycosylation types and sites on L. vannamei hemocyanin as well as how this relates to its immune-related activities.…”

Section: Litopenaeus Vannameimentioning

confidence: 99%

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Identification and characterization of glycosylation sites on Litopenaeus vannamei hemocyanin

Zhang

Aweya

et al. 2019

FEBS Letters

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The respiratory glycoprotein hemocyanin has been implicated in immune‐related functions. Using lectin blotting, we show that the binding of shrimp (Litopenaeus vannamei) hemocyanin to concanavalin A decreases markedly with O‐glycosidase treatment but not with PNGase F. Twelve O‐glycosylation sites, three on the large hemocyanin subunit and nine on the small hemocyanin subunit (HMCs), were identified by LC‐MS/MS. Importantly, when the glycosylation sites at Thr‐537, Ser‐539, and Thr‐542 on the C terminus of HMCs were replaced with alanine, the resultant mutant hemocyanin had reduced carbohydrate content, coupled with a fourfold reduction in bacterial agglutination and 0.2‐fold reduction in antibacterial activities toward Vibrio parahaemolyticus and Staphylococcus aureus. These results suggest that the glycosylation sites on shrimp hemocyanin are closely related to its immunological functions.

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Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Litopenaeus Vannameimentioning

confidence: 99%

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Identification and characterization of glycosylation sites on Litopenaeus vannamei hemocyanin

Zhang

Aweya

et al. 2019

FEBS Letters

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“…Numerous studies have shown that shrimp haemocyanin has many immune‐related functions, including antiviral (Zhang et al, 2004; Chongsatja et al, 2007; Lei et al, 2008), phenoloxidase (Coates et al, 2013; Bris et al, 2016), agglutination (Fang et al, 2011), antibacterial (Zhang et al, 2006; Fang et al, 2011; Zhang et al, 2017), haemolytic (Zhang et al, 2009; Yan et al, 2011), anti‐tumour (Zheng et al, 2016; Liu et al, 2017) and many other immunological functions, which could be one of the reasons for its high abundance in shrimp haemolymph (Depledge & Bjerregaard, 1989; Mullaivanam Ramasamy et al, 2017). Thus, it is believed that when shrimp are invaded by pathogens under high ammonia stress, some haemocyanin degrades into antimicrobial peptides by proteases such as trypsin (Destoumieux‐Garzon et al, 2001; Li et al, 2018a) to protect shrimp from the pathogens.…”

Section: Shrimp Haemocyanin and Ammonia Stress In Immune Responsementioning

confidence: 99%

Effects of ammonia on shrimp physiology and immunity: a review

Zhao

Yao

et al. 2020

Reviews in Aquaculture

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103

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The rapid expansion in shrimp farming comes with problems such as inadequate culture technology, disease outbreak, water pollution and other environmental degradation‐related problems. Prominent among these is the level of ammonia, which has been rising steadily in the last couple of years, mainly due to farming practices and other anthropogenic activities, and therefore has had consequent effects on marine life, especially shrimp. While optimal ammonia levels are a good source of nitrogen for marine phytoplankton that increases the level of dissolved oxygen in water as well serves as food for shrimp, high levels of ammonia are harmful to shrimp. When shrimp pond ammonia levels go beyond the tolerance limits, it inhibits chitinase expression, moulting, growth, phenoloxidase and haemolymph antimicrobial activity, thereby attenuating shrimp innate immune response. In this review, we bring together recent information on the effects of ammonia stress on the growth, physiology, biochemistry, ammonia‐metabolizing enzymes and immunity of shrimp. We also propose areas of research that can be explored in the breeding of new ammonia‐tolerant and disease‐resistant shrimp with robust immune and/or physiological systems that could withstand environmental stress and pathogens.

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“…Glycans also play an important role in cardiovascular disease, microbial and viral pathogenesis, development of tumors, tissue repair, strengthening of the immune system, and others [ 2 , 3 , 4 ]. As demonstrated by various studies [ 5 , 6 ], hemocyanins have gained a large amount scientific interest due to their interesting glycoprotein structures.…”

Section: Introductionmentioning

confidence: 99%

De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs

et al. 2020

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A number of studies have shown that glycosylation of proteins plays diverse functions in the lives of organisms, has crucial biological and physiological roles in pathogen–host interactions, and is involved in a large number of biological events in the immune system, and in virus and bacteria recognition. The large amount of scientific interest in glycoproteins of molluscan hemocyanins is due not only to their complex quaternary structures, but also to the great diversity of their oligosaccharide structures with a high carbohydrate content (2–9%). This great variety is due to their specific monosaccharide composition and different side chain composition. The determination of glycans and glycopeptides was performed with the most commonly used methods for the analysis of biomolecules, including peptides and proteins, including Matrix Assisted Laser Desorption/Ionisation–Time of Flight (MALDI-TOF-TOF), Liquid Chromatography - Electrospray Ionization-Mass Spectrometry (LC/ESI-MS), Liquid Chromatography (LC-Q-trap-MS/MS) or Nano- Electrospray Ionization-Mass Spectrometry (nano-ESI-MS) and others. The molluscan hemocyanins have complex carbohydrate structures with predominant N-linked glycans. Of interest are identified structures with methylated hexoses and xyloses arranged at different positions in the carbohydrate moieties of molluscan hemocyanins. Novel acidic glycan structures with specific glycosylation positions, e.g., hemocyanins that enable a deeper insight into the glycosylation process, were observed in Rapana venosa, Helix lucorum, and Haliotis tuberculata. Recent studies demonstrate that glycosylation plays a crucial physiological role in the immunostimulatory and therapeutic effect of glycoproteins. The remarkable diversity of hemocyanin glycan content is an important feature of their immune function and provides a new concept in the antibody–antigen interaction through clustered carbohydrate epitopes.

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Glycosylation of hemocyanin in Litopenaeus vannamei is an antibacterial response feature

Cited by 36 publications

References 34 publications

Identification and characterization of glycosylation sites on Litopenaeus vannamei hemocyanin

Identification and characterization of glycosylation sites on Litopenaeus vannamei hemocyanin

Effects of ammonia on shrimp physiology and immunity: a review

De Novo Structural Determination of the Oligosaccharide Structure of Hemocyanins from Molluscs

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