Glycosylinositol phosphoceramide-specific phospholipase D activity catalyzes transphosphatidylation

Hasi, Rumana Yesmin; Miyagi, Makoto; Morito, Katsuya; Ishikawa, Toshiki; Kawai‐Yamada, Maki; Imai, Hideki; Fukuta, Tatsuya; Kogure, Kentaro; Kanemaru, Kaori; Hayashi, Junji; Kawakami, Retsuo; Tanaka, Tamotsu

doi:10.1093/jb/mvz056

Cited by 9 publications

(10 citation statements)

References 24 publications

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“…6D). These results are comparable to those of our previous studies on the activity of partially purified GIPC‐PLD activity from cabbage [20].…”

Section: Resultssupporting

confidence: 91%

“…Here, we identify that the enzyme responsible for the GIPC‐PLD activity in radish is nonspecific phospholipase C3 (NPC3). The recombinant NPC3 protein of radish, produced by a heterologous expression system with Escherichia coli , showed essentially the same enzymatic nature as the previously characterized GIPC‐PLD in cabbage, radish and Arabidopsis thaliana [18–20].…”

Section: Figmentioning

confidence: 78%

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Nonspecific phospholipase C3 of radish has phospholipase D activity towards glycosylinositol phosphoceramide

et al. 2022

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Glycosylinositol phosphoceramide (GIPC) is a major sphingolipid in the plasma membranes of plants. Previously, we found an enzyme activity that produces phytoceramide 1-phosphate (PC1P) by hydrolysis of the D position of GIPC in cabbage and named this activity as GIPC-phospholipase D (PLD). Here, we purified GIPC-PLD by sequential chromatography from radish roots. Peptide mass fingerprinting analysis revealed that the potential candidate for GIPC-PLD protein was nonspecific phospholipase C3 (NPC3), which has not been characterized as a PLD. The recombinant NPC3 protein obtained by heterologous expression system in Escherichia coli produced PC1P from GIPC and showed essentially the same enzymatic properties as those we characterized as GIPC-PLD in cabbage, radish and Arabidopsis thaliana. From these results, we conclude that NPC3 is one of the enzymes that degrade GIPC.

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“…6D). These results are comparable to those of our previous studies on the activity of partially purified GIPC‐PLD activity from cabbage [20].…”

Section: Resultssupporting

confidence: 91%

Section: Figmentioning

confidence: 78%

Nonspecific phospholipase C3 of radish has phospholipase D activity towards glycosylinositol phosphoceramide

et al. 2022

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“…A phospholipase D activity has been detected in cabbage and in A. thaliana 21 . This GIPC-PLD prefers GIPC containing two sugars 22 which is consistent with the fact that Man-GlcA-Ino is the most highly produced in our conditions. However, we cannot rule out another scenario that would imply a phospholipase C activity and a subsequent phosphorylase.…”

Section: Introductionsupporting

confidence: 90%

Inositolphosphate glycans and a fucosylated xyloglucan oligosaccharide are accumulated upon Arabidopsis thaliana/ Botrytis cinerea infection

Voxeur

Sechet

Vernhettes

2021

Preprint

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In mammals, insulin is involved in controlling blood glucose levels and its role in modulating immunity is being more and more documented. This hormone promotes the release of inositolphosphate glycans (IPG) which act as mediators. In plants, one IG has already been identified in plant culture cells (Smith and Fry, 1999; Smith et al., 1999) but, to our knowledge, no IPG have been yet identified. Here, we discovered 7 IPG that are accumulated upon Arabidopsis thaliana-Botrytis cinerea interaction, concomitantly with oligogalacturonides and a fucosylated xyloglucan oligosaccharide. Further structural characterization showed that they come from the hydrolysis of polar heads of Serie A to H glycosyl inositol phosphoryl ceramides presumably via a phospholipase C activity. Taken together with the emerging role of insulin as immune regulator, these results question the role of IPG as damage associated molecular pattern both in animal and plant kingdoms.

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“…In cabbage leaves, a GIPC-specific phospholipase D (GIPC-PLD) was found to cleave at the D position of the ester linkage between inositol and phosphate to produce phytoceramide 1-phosphate (PC1P) ( Hasi et al., 2019 ). The enzyme exhibits a substrate preference for GIPCs that contain two sugars ( Hasi et al., 2019 ). In addition, a nonspecific phospholipase C4 (NPC4) was recently reported to hydrolyze GIPCs during phosphate deficiency ( Yang et al., 2021 ).…”

Section: Sphingolipid Metabolismmentioning

confidence: 99%

Sphingolipid metabolism, transport, and functions in plants: Recent progress and future perspectives

Liu

Hou

Bao

et al. 2021

Plant Communications

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Sphingolipids, which comprise membrane systems together with other lipids, are ubiquitous in cellular organisms. They show a high degree of diversity across plant species and vary in their structures, properties, and functions. Benefiting from the development of lipidomic techniques, over 300 plant sphingolipids have been identified. Generally divided into free long-chain bases (LCBs), ceramides, glycosylceramides (GlcCers) and glycosyl inositol phosphoceramides (GIPCs), plant sphingolipids exhibit organized aggregation within lipid membranes to form raft domains with sterols. Accumulating evidence has revealed that sphingolipids obey certain trafficking and distribution rules and confer unique properties to membranes. Functional studies using sphingolipid biosynthetic mutants demonstrate that sphingolipids participate in plant developmental regulation, stimulus sensing, and stress responses. Here, we present an updated metabolism/degradation map and summarize the structures of plant sphingolipids, review recent progress in understanding the functions of sphingolipids in plant development and stress responses, and review sphingolipid distribution and trafficking in plant cells. We also highlight some important challenges and issues that we may face during the process of studying sphingolipids.

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Glycosylinositol phosphoceramide-specific phospholipase D activity catalyzes transphosphatidylation

Cited by 9 publications

References 24 publications

Nonspecific phospholipase C3 of radish has phospholipase D activity towards glycosylinositol phosphoceramide

Nonspecific phospholipase C3 of radish has phospholipase D activity towards glycosylinositol phosphoceramide

Inositolphosphate glycans and a fucosylated xyloglucan oligosaccharide are accumulated upon Arabidopsis thaliana/ Botrytis cinerea infection

Sphingolipid metabolism, transport, and functions in plants: Recent progress and future perspectives

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