Glycosyltransferase-catalyzed synthesis of bioactive oligosaccharides

Expanding the Nucleotide and Sugar 1-Phosphate Promiscuity of Nucleotidyltransferase RmlA via Directed Evolution

“…The availability of diverse sugar nucleotides is key to this process, and given the challenges associated with their chemical synthesis (13)(14)(15), the enzymatic synthesis of sugar nucleotides is an attractive alternative (16,17). Within this context, certain sugar-1-phosphate nucleotidyltransferases display some flexibility toward non-native sugar 1-phosphates and, in some cases, even non-native NTPs (18 -25), the promiscuity of which can be further expanded via protein engineering (26 -28).…”

Molecular mechanisms and biological role ofCampylobacter jejuniattachment to host cells

“…Remarkably, preliminary experiments did show inhibitory effect of fucosyl-oligosaccharides on Campylobacter attachment and colonisation using a mice model of infection [73]. The synthesis and usage of specific oligosaccharides as inhibitors of bacterial adhesion is a highly promising research direction [74]. How-ever, for most putative adhesins the receptors still remain unknown.…”

Carbohydrate drugs: current status and development prospect

“…On the other hand, enzymatic approaches with high efficiency and selectivity are usually limited by the sources of the enzymes desired. Consequently, chemoenzymatic synthesis which combines the flexibility of the chemical method and the efficiency and selectivity of the enzymatic method is considered to be one of the most efficient approaches for complex oligosaccharide preparation (82,83). A disialyl tetrasaccharide epitope has been regioselectively synthesized based on a chemical manipulation strategy mediated by a bacterial sialyltransferase Pd2,6ST (84).…”