Glycoxidized low-density lipoprotein enhances monocyte chemoattractant protein-1 mRNA expression in human umbilical vein endothelial cells: Relation to lysophosphatidylcholine contents and inhibition by nitric oxide donor

Sonoki, Kazuo; Yoshinari, Mototaka; Iwase, Masanori; Iino, Kenzo; Ichikawa, Kojiro; Ohdo, Shigehiro; Higuchi, Shun; Iida, Mitsuo

doi:10.1053/meta.2002.34703

Cited by 34 publications

(23 citation statements)

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“…Moreover, the previous studies suggest that NF-kB activation is an essential step in the overproduction of MCP-1 induced by oxidized LDL or glycated and oxidized LDL in vascular cells (Sonoki et al 2002, Renier et al 2003. Thus, we next determined whether PEDF reduces HOG-LDL-induced MCP-1 secretion through the inhibition of NF-kB activation.…”

Section: Hog-ldl Induces Mcp-1 Secretion In Retinal Pericytes and Pementioning

confidence: 99%

“…NF-kB activation in retinal pericytes was induced by oxidized LDL, and the effect was inhibited by PEDF NF-kB activation is an important transcriptional factor regulating the expression of a variety of pro-inflammatory cytokines and adhesion molecules, including MCP-1 (Sonoki et al 2002, Renier et al 2003. Moreover, the previous studies suggest that NF-kB activation is an essential step in the overproduction of MCP-1 induced by oxidized LDL or glycated and oxidized LDL in vascular cells (Sonoki et al 2002, Renier et al 2003.…”

Section: Hog-ldl Induces Mcp-1 Secretion In Retinal Pericytes and Pementioning

confidence: 99%

“…MCP-1 belongs to the CC chemokine family and is a potent chemoattractant for monocytes. The critical role of MCP-1 in diabetic vascular complications has been intensively studied and established in cardiovascular (macrovascular) diseases in diabetes (Sonoki et al 2002, Renier et al 2003. In diabetic retinopathy, MCP-1 levels in the vitreous are significantly elevated in patients with proliferative diabetic retinopathy (Abu El-Asrar et al 1997, Mitamura et al 2001, Hernández et al 2005.…”

Section: Introductionmentioning

confidence: 99%

“…Oxidized low-density lipoprotein (LDL)-mediated inflammation and vascular damage are recognized as central to the pathogenesis of atherosclerosis and to the accelerated atherosclerosis of diabetes (Sonoki et al 2002, Renier et al 2003). An important mechanism for the deleterious effect of oxidized LDL on vascular function is to induce MCP-1 production and monocyte/macrophage recruitment (Sonoki et al 2002, Renier et al 2003.…”

Section: Introductionmentioning

confidence: 99%

“…An important mechanism for the deleterious effect of oxidized LDL on vascular function is to induce MCP-1 production and monocyte/macrophage recruitment (Sonoki et al 2002, Renier et al 2003. In diabetic retina, the disrupted blood-retinal barrier and increased vascular permeability result in extravasation and accumulation of LDL in the perivascular space, where it undergoes additional glycation and extensive oxidation.…”

Section: Introductionmentioning

confidence: 99%

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Pigment epithelium-derived factor mitigates inflammation and oxidative stress in retinal pericytes exposed to oxidized low-density lipoprotein

Zhang¹,

Wang²,

Dashti³

et al. 2008

Journal of Molecular Endocrinology

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Oxidized and/or glycated low-density lipoprotein (LDL) may mediate capillary injury in diabetic retinopathy. The mechanisms may involve pro-inflammatory and pro-oxidant effects on retinal capillary pericytes. In this study, these effects, and the protective effects of pigment epithelium-derived factor (PEDF), were defined in a primary human pericyte model. Human retinal pericytes were exposed to 100 mg/ml native LDL (N-LDL) or heavily oxidized glycated LDL (HOG-LDL) with or without PEDF at 10-160 nM for 24 h. To assess pro-inflammatory effects, monocyte chemoattractant protein-1 (MCP-1) secretion was measured by ELISA, and nuclear factor-kB (NF-kB) activation was detected by immunocytochemistry. Oxidative stress was determined by measuring intracellular reactive oxygen species (ROS), peroxynitrite (ONOO K ) formation, inducible nitric oxide synthase (iNOS) expression, and nitric oxide (NO) production. The results showed that MCP-1 was significantly increased by HOG-LDL, and the effect was attenuated by PEDF in a dose-dependent manner. PEDF also attenuated the HOG-LDL-induced NF-kB activation, suggesting that the inhibitory effect of PEDF on MCP-1 was at least partially through the blockade of NF-kB activation. Further studies demonstrated that HOG-LDL, but not N-LDL, significantly increased ONOO K formation, NO production, and iNOS expression. These changes were also alleviated by PEDF. Moreover, PEDF significantly ameliorated HOG-LDL-induced ROS generation through up-regulation of superoxide dismutase 1 expression. Taken together, these results demonstrate proinflammatory and pro-oxidant effects of HOG-LDL on retinal pericytes, which were effectively ameliorated by PEDF. Suppressing MCP-1 production and thus inhibiting macrophage recruitment may represent a new mechanism for the salutary effect of PEDF in diabetic retinopathy and warrants more studies in future.

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Section: Hog-ldl Induces Mcp-1 Secretion In Retinal Pericytes and Pementioning

confidence: 99%

Section: Hog-ldl Induces Mcp-1 Secretion In Retinal Pericytes and Pementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Pigment epithelium-derived factor mitigates inflammation and oxidative stress in retinal pericytes exposed to oxidized low-density lipoprotein

Zhang¹,

Wang²,

Dashti³

et al. 2008

Journal of Molecular Endocrinology

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Analysis of in vitro oxidized human LDL phospholipids by solid‐phase extraction and micellar electrokinetic capillary chromatography

Yang

Chong

Tsai

et al. 2011

Biomedical Chromatography

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Phospholipids of in vitro oxidized human low-density lipoproteins (LDL) were separated by two different solid-phase extraction (SPE) methods. One of the two methods was designed to test the effects of gradient elution. This SPE method isolated more phospholipids from in vitro oxidized LDL than the other one according to the results of liquid chromatography and electrospray ionization mass spectrometry (LC ESI-MS) analysis. A micellar electrokinetic capillary chromatography (MEKC) method was also used to analyze phospholipids separated by SPE. The results of MEKC and LC ESI-MS were consistent for the major phospholipid classes, including PC, lysoPC, PE, PI and PS. The MEKC profiles showed significant differences for native and oxidized LDL phospholipids. Therefore, the unique combination of SPE and MEKC methods showed dramatic distinctions between native and in vitro oxidized human LDL phospholipids. The combination also shows great potential for rapid analysis of in vivo oxidized human LDL phospholipids in the future.

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Separation of total lipids on human lipoproteins using surfactant‐coated multiwalled carbon nanotubes as pseudostationary phase in capillary electrophoresis

Chen

Yang

et al. 2013

Electrophoresis

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Surfactant-coated multiwalled carbon nanotubes (MWNTs) were used as pseudostationary phase (PSP) in CE to investigate the total lipids of high-density lipoproteins and low-density lipoproteins. To optimize the CE conditions, several experimental factors including carbon nanotube concentration, bile salt concentration, sodium phosphate (PB) concentration, organic modifier concentration and buffer pH value have been examined. In addition, the CE capillary temperature and applied voltage have also been examined. The optimal separation buffer selected was a mixture of 3.2 mg/L MWNT, 50 mM bile salt, 10 mM PB, 20% 1-propanol, pH 9.5. The optimal capillary temperature and applied voltage selected were 50°C and 20 kV, respectively. Phosphatidyl choline (PC) has been used as a model analyte and investigated by the optimal CE method. The linear range for PC was 0.1-3 mg/mL with a correlation coefficient of 0.9934, and the concentration LOD was 0.055 mg/mL. The optimal CE method has been used to characterize the total lipids of high-density lipoprotein and low-density lipoprotein. At absorbance 200 nm, one major peak and two or three minor peaks showed for the total lipids of lipoproteins within 13 minutes. Resolutions of the total lipids were enhanced using surfactant-coated MWNTs as PSPs in the CE separation buffer. However, resolutions of the total lipids were not enhanced using surfactant-coated single-walled carbon nanotubes as PSPs in the CE separation buffer.

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Glycoxidized low-density lipoprotein enhances monocyte chemoattractant protein-1 mRNA expression in human umbilical vein endothelial cells: Relation to lysophosphatidylcholine contents and inhibition by nitric oxide donor

Cited by 34 publications

References 0 publications

Pigment epithelium-derived factor mitigates inflammation and oxidative stress in retinal pericytes exposed to oxidized low-density lipoprotein

Pigment epithelium-derived factor mitigates inflammation and oxidative stress in retinal pericytes exposed to oxidized low-density lipoprotein

Analysis of in vitro oxidized human LDL phospholipids by solid‐phase extraction and micellar electrokinetic capillary chromatography

Separation of total lipids on human lipoproteins using surfactant‐coated multiwalled carbon nanotubes as pseudostationary phase in capillary electrophoresis

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