Glycycoumarin from Glycyrrhizae Radix acts as a potent antispasmodic through inhibition of phosphodiesterase 3

Sato, Yuji; Akao, Teruaki; He, Juan; Nishimura, Hiroshi; Kuraishi, Yasushi; Morota, Takashi; Asano, Takayuki; Tani, Tohru

doi:10.1016/j.jep.2005.11.017

Cited by 56 publications

(46 citation statements)

References 15 publications

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“…3). Moreover, isoliquiritigenin increased neither the content of cAMP nor cGMP in mouse jejunum (data not shown), wheraeas glycycoumarin increased the content of cAMP 6) to an extent almost equivalent to that obtained with papaverine in guinea pig ileum. 16) Although isoliquiritigenin and glycycoumarin are potent inhibitors of PDEs, 13) the former (IC 50 180 mM) is much weaker, by two orders of magnitude, than the latter (IC 50 7 mM).…”

Section: Discussionmentioning

confidence: 82%

Isoliquiritigenin, One of the Antispasmodic Principles of Glycyrrhiza Ularensis Roots, Acts in the Lower Part of Intestine

Sato

Nagai

et al. 2007

Biological & Pharmaceutical Bulletin

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Section: Discussionmentioning

confidence: 82%

Isoliquiritigenin, One of the Antispasmodic Principles of Glycyrrhiza Ularensis Roots, Acts in the Lower Part of Intestine

Sato

Nagai

et al. 2007

Biological & Pharmaceutical Bulletin

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“…For example, three metabolites were derived from glycycoumarin, which was present in LWE in a noticeable amount. Glycycoumarin had been reported to show potent antispasmodic activities on carbamylcholine-induced mouse jejunum contraction (Sato et al, 2006). A famous TCM formula, Shaoyao-Gancao decoction (composed of licorice and peony root), is widely used to treat spasm and abdominal pain (Katsura, 1995).…”

Section: Discussionmentioning

confidence: 99%

From Single Compounds to Herbal Extract: A Strategy to Systematically Characterize the Metabolites of Licorice in Rats

Cheng¹,

Qiao²,

Wang³

et al. 2011

Drug Metab Dispos

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ABSTRACT:Because of the complicated chemical composition of traditional Chinese medicines (TCMs), their metabolic study has been a great challenge, especially when they are used in the traditional way, i.e., clinical oral dose of water decoction. Poor understanding of metabolic pathways and too low metabolite concentrations to be detected in biosamples are the major hurdles. In the present work, a three-step strategy was proposed to systematically characterize in vivo metabolites of TCMs at a normal clinical dosage. Licorice, one of the most popular TCMs, was studied as a model. First, 10 representative compounds of licorice were administered to rats separately. A total of 68 metabolites were characterized by highperformance liquid chromatography coupled with diode-array detection and electrospray ionization tandem mass spectrometry and liquid chromatography (LC)/quadrupole time-of-flight-mass spectrometry (MS) analyses, together with enzyme hydrolysis. Among these, 13 compounds were confirmed by comparison with reference standards, including the 10 administered licorice compounds. Second, a high dose (equivalent to 20-fold clinical dosage) of licorice water extract was administered, and 22 more metabolites were characterized. Finally, these metabolites (including constituents of licorice) were determined by a highly sensitive and selective LC/selected reaction monitoring-MS method when the licorice water decoction was orally administered to rats at a clinical dosage (0.9 g crude drug/kg). A total of 42 metabolites in plasma and 62 metabolites in urine were detected. This is the first attempt to fully profile the in vivo metabolites of licorice at a normal clinical dosage.

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“…12) Recently, glycycoumarin has been characterized as the antispasmodic ingredient of licorice through experiments with carbachol-induced contracted mice jejunum. 7) Based on these data, the ex vivo method of using isolated jejunum to screen for bioactive properties of extracts appears to be of utility in defining the active ingredients of licorice.…”

Section: Discussionmentioning

confidence: 99%

“…Evaluation of carbachol-induced contractile response of isolated mice jejunum was performed as previously described. 7) Briefly, a 1 cm segment of jejunum was isolated from male ICR mice (7-10 weeks old). After maximal contraction was evoked by acetylcholine (1 mM), the segments were washed and allowed to equilibrate for another 45 min, and then were exposed to carbachol (1 mM).…”

Section: )mentioning

confidence: 99%

“…1) has been isolated as an antispasmodic ingredient of licorice, as demonstrated by its ability to relax carbamylcholine (carbachol)-induced contraction of isolated mice jejunum. 7) Thus, the goal of the present study was to clarify the bio-equivalency between the cultivated G. uralensis roots and concurrent licorice by assessing glycycoumarin contents and the relaxant effects on isolated mice jejunum of each preparation.…”

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Pharmaceutical Evaluation of Cultivated Glycyrrhiza uralensis Roots in Comparison of Their Antispasmodic Activity and Glycycoumarin Contents with Those of Licorice

Nagai

Yamamoto

Sato

et al. 2006

Biological & Pharmaceutical Bulletin

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In China, the collection of wild Glycyrrhiza uralensis, one of the raw materials of Chinese licorice, has been restricted to prevent desertification. To compensate for the reduced supply of wild Glycyrrhiza plants, cultivation programs of G. uralensis have been initiated in eastern Inner Mongolia. The goal of the present study was to compare the chemical and pharmacological properties of cultivated G. uralensis roots to those of licorice prepared from wild Glycyrrhiza plants. The antispasmodic effect of boiled water extract of 4-year-old cultivated G. uralensis roots and licorice on carbachol-induced contraction in mice jejunum was similar (ED 50 : 134؎21 m mg/ml vs. 134؎16 m mg/ml). In addition, glycycoumarin content, which is an antispasmodic and species-specific ingredient of G. uralensis, was similar when comparing the boiled water extracts of 4-year-old cultivated roots and licorice (0.10؎0.02% vs. 0.10؎0.06%). These data suggest that cultivated G. uralensis roots may be an adequate replacement for the generation of licorice in the context of the restriction of wild Glycyrrhiza plant collection.

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Glycycoumarin from Glycyrrhizae Radix acts as a potent antispasmodic through inhibition of phosphodiesterase 3

Cited by 56 publications

References 15 publications

Isoliquiritigenin, One of the Antispasmodic Principles of Glycyrrhiza Ularensis Roots, Acts in the Lower Part of Intestine

Isoliquiritigenin, One of the Antispasmodic Principles of Glycyrrhiza Ularensis Roots, Acts in the Lower Part of Intestine

From Single Compounds to Herbal Extract: A Strategy to Systematically Characterize the Metabolites of Licorice in Rats

Pharmaceutical Evaluation of Cultivated Glycyrrhiza uralensis Roots in Comparison of Their Antispasmodic Activity and Glycycoumarin Contents with Those of Licorice

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