Glycyrrhiza glabra (Linn.) and Lavandula officinalis (L.) cell suspension cultures-based biotransformation of β-artemether

Abstract

The biotransformation of β-artemether (1) by cell suspension cultures of Glycyrrhiza glabra and Lavandula officinalis is reported here for the first time. The major biotransformed product appeared as a grayish-blue color spot on thin-layer chromatography (TLC) with transparent crystal-like texture. Based on its infrared (IR) and (1)H nuclear magnetic resonance (NMR) spectra, the product was characterized as a tetrahydrofuran (THF)-acetate derivative (2). The highest conversion efficiencies of 57 and 60% were obtained when 8-9-day-old cell suspensions of G. glabra and L. officinalis were respectively fed with 4-7 mg of compound 1 in 40 ml of medium per culture and the cells were harvested after 2-5 days of incubation. The addition of compound 1 at the beginning of the culture cycle caused severe growth depression in a dose-dependent manner, resulting in poor bioconversion efficiency of ~25% at 2-5 mg/culture dose only.

In vitro antimalarial studies of novel artemisinin biotransformed products and its derivatives

“…In the continuation of our earlier biotransformation studies on bioactive compounds (Patel et al, 2010(Patel et al, , 2011 http://dx.doi.org/10.1016/j.phytochem.2014.08.004 0031-9422/Ó 2014 Elsevier Ltd. All rights reserved.…”

Biodiversity of Lianas

“…The major biotransformation product was characterized as a tetrahydrofuran (THF) -acetate derivative. This was an effort to synthesize more effi cient artemisinin derivatives/analogs with improved clinical effi ciency (Patel et al 2011 ). Other report on Glycyrrhiza showed that extracts of its cell culture possessed a similar anti-infl ammatory effect to those of fi eld-cultivated equivalent, through the enhancement of the superoxide dismutase activity of plasma and liver tissues.…”