Glycyrrhiza uralensis Fisch. alleviates dextran sulfate sodium-induced colitis in mice through inhibiting of NF-κB signaling pathways and modulating intestinal microbiota

Shi, Guoping; Kong, Jinrong; Wang, Yunlai; Xuan, Zihua; Xu, Fan

doi:10.1016/j.jep.2022.115640

Cited by 24 publications

(12 citation statements)

References 47 publications

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“…In previous studies, we investigated the effects of licorice on DSS‐induced colitis in mice by inhibiting the nuclear facter‐kappa B signaling pathway and modulating intestinal microbiota 18 . Levels of DSS‐induced UC were observed in licorice‐treated mice, validating the ameliorative effects of licorice on UC.…”

Section: Discussionmentioning

confidence: 74%

“…Eluate was monitored by a diode array detector at a wavelength of 254 nm (20 µl, 1.0 ml/m, 30°C). HPLC condition reference Shi et al 18 …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…After 7 days, the mice were randomly divided into 3% DSS group, mesalazine group (200 mg/kg/day), licorice low, middle and high group (4, 8, 16 g/kg/day), the drug was given orally continuously for 7 days (normal group was given saline solution). UC model reference Shi et al 18 After 7 days of 3% DSS for UC modeling, licorice and mesalazine were gavaged for 7 days, and the body weight of the mice was recorded daily. On the 15th day, after the mice had been killed by taking blood under anesthesia, the spleens were removed and weighed for recording.…”

Section: Establishment Of Ucmentioning

confidence: 99%

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Licorice protects against ulcerative colitis via the Nrf2/PINK1‐mediated mitochondrial autophagy

Kong

Xiang

Shi

et al. 2022

Immunity Inflam & Disease

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Purpose Study of the effects and mechanisms of licorice in the treatment of ulcerative colitis (UC) from the perspective of mitochondrial autophagy. Methods BALB/C mice were induced with 3% dextran sodium sulfate to build an animal model of UC. After 7 days of modeling, different doses of licorice were administered for 7 days. Hematoxylin and eosin staining is used to detect pathological changes in the colon. Mitochondrial membrane potentials and reactive oxygen species (ROS) contents were detected by flow cytometry, and autophagy of mitochondria was observed by transmission electron microscopy. Determination of inflammatory cytokines by enzyme‐linked immunosorbent assay. The oxidizing factors are detected by the kits. Western blot analysis was used to detect expressions for nuclear factor called erythropoietin (Nrf2), pten‐induced protein kinase 1 (PINK1), Parkin, HO‐1, P62, and LC3. Results Licorice improved the pathological condition of UC mice, increasing the mitochondrial membrane potential and decreasing the ROS content. Promotes the emergence of autophagosomes and autophagosomes. The contents of interleukin (IL)‐1β, IL‐6, IL‐17, and tumor necrosis factor‐alpha were downregulated, the contents of superoxide dismutase and glutathione peroxidase were upregulated and the contents of malondialdehyde were downregulated. In addition, licorice promotes the expression of Nrf2, PINK1, Parkin, HO‐1, P62, and LC3. Conclusion Licorice was shown to reduce levels of inflammatory factors and oxidative stress in mice with UC, possibly by promoting mitochondrial autophagy through the activation of the Nrf2/PINK1 pathway.

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Section: Discussionmentioning

confidence: 74%

“…Eluate was monitored by a diode array detector at a wavelength of 254 nm (20 µl, 1.0 ml/m, 30°C). HPLC condition reference Shi et al 18 …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Establishment Of Ucmentioning

confidence: 99%

See 2 more Smart Citations

Licorice protects against ulcerative colitis via the Nrf2/PINK1‐mediated mitochondrial autophagy

Kong

Xiang

Shi

et al. 2022

Immunity Inflam & Disease

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“…Glycyrrhiza uralensis Fisch. (GC) is a herbaceous perennial legume copiously cultivated in many places worldwide, more commonly used for detoxification and anti‐inflammatory 11–13 . GC contains a variety of bioactive components, mainly triterpenoids and flavonoids 14,15 .…”

Section: Introductionmentioning

confidence: 99%

Exploring the pharmacological mechanism of Glycyrrhiza uralensis against KOA through integrating network pharmacology and experimental assessment

Xu,

Sun,

Qiu

et al. 2024

J Cellular Molecular Medi

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Knee osteoarthritis (KOA), a major health and economic problem facing older adults worldwide, is a degenerative joint disease. Glycyrrhiza uralensis Fisch. (GC) plays an integral role in many classic Chinese medicine prescriptions for treating knee osteoarthritis. Still, the role of GC in treating KOA is unclear. To explore the pharmacological mechanism of GC against KOA, UPLC‐Q‐TOF/MS was conducted to detect the main compounds in GC. The therapeutic effect of GC on DMM‐induced osteoarthritic mice was assessed by histomorphology, μCT, behavioural tests, and immunohistochemical staining. Network pharmacology and molecular docking were used to predict the potential targets of GC against KOA. The predicted results were verified by immunohistochemical staining Animal experiments showed that GC had a protective effect on DMM‐induced KOA, mainly in the improvement of movement disorders, subchondral bone sclerosis and cartilage damage. A variety of flavonoids and triterpenoids were detected in GC via UPLC‐Q‐TOF/MS, such as Naringenin. Seven core targets (JUN, MAPK3, MAPK1, AKT1, TP53, RELA and STAT3) and three main pathways (IL‐17, NF‐κB and TNF signalling pathways) were discovered through network pharmacology analysis that closely related to inflammatory response. Interestingly, molecular docking results showed that the active ingredient Naringenin had a good binding effect on anti‐inflammatory‐related proteins. In the verification experiment, after the intervention of GC, the expression levels of pp65 and F4/80 inflammatory indicators in the knee joint of KOA model mice were significantly downregulated. GC could improve the inflammatory environment in DMM‐induced osteoarthritic mice thus alleviating the physiological structure and dysfunction of the knee joint. GC might play an important role in the treatment of knee osteoarthritis.

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Mitigating impact of Glycyrrhiza glabra on virulent Newcastle disease virus challenge in chickens: clinical studies, histopathological alterations and molecular docking

Haleem,

Gaballa,

El-Far

et al. 2024

Vet Res Commun

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Background Newcastle disease (ND) is widely regarded as one of the most virulent and destructive viral infections that create chaos in the poultry industry and cause widespread epidemics and consequentially debilitating economic losses on a global scale in terms of chicken products. The current experiment evaluates the protective effect of Glycyrrhiza glabra ( G. glabra) against the Newcastle disease virus (NDV) in chickens. Ninety (90) 1-day-old SPF chicks were treated according to ethical approval (BUFVTM 05-02-22) as follows (1) non-treated non-challenged control group; (2) NDV group: Challenged with genotype VII ND virus; and (3) LE/NDV group: Challenged with the virus and intermittently treated with powdered extract of G. glabra roots (LE) in drinking water (0.5 g/L) before and after viral challenge. Result The water medication of NDV-challenged chicks has resulted in a significant decrease in the severity of clinical symptoms, morbidity, and mortality rates, as well as the quantity of virus shed, compared with the NDV group. Treatment with LE has led to a significant reduction in serum ALT and AST activities, blood glucose level, urea, and creatinine, and significant restoration of serum proteins. In addition, the treatment has resulted in a decrease in MDA and NO levels, as well as an increase in T-SOD and catalase activities compared with untreated challenged chicks. LE decreased IFN-γ and TLR-3 gene expression in comparison with the NDV group. The treated challenged birds had fewer macroscopically detectable lesions in their respiratory, digestive, and lymphoid organs than the untreated challenged birds. Microscopically, the LE/NDV group exhibited mild to moderate pathological changes in the respiratory and digestive systems as well as lymphoid tissues, in contrast to the NDV group, which exhibited severe pathological changes. Furthermore, molecular docking assessment proved the efficacy of G. glabra against viral proliferation and invasion. Conclusion We concluded that Glycyrrhiza glabra powdered extract at a dose of 0.5 g/L drinking water can effectively mitigate the debilitating effects of Newcastle disease in chickens.

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Glycyrrhiza uralensis Fisch. alleviates dextran sulfate sodium-induced colitis in mice through inhibiting of NF-κB signaling pathways and modulating intestinal microbiota

Cited by 24 publications

References 47 publications

Licorice protects against ulcerative colitis via the Nrf2/PINK1‐mediated mitochondrial autophagy

Licorice protects against ulcerative colitis via the Nrf2/PINK1‐mediated mitochondrial autophagy

Exploring the pharmacological mechanism of Glycyrrhiza uralensis against KOA through integrating network pharmacology and experimental assessment

Mitigating impact of Glycyrrhiza glabra on virulent Newcastle disease virus challenge in chickens: clinical studies, histopathological alterations and molecular docking

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