Glycyrrhizin production by in vitro cultured Glycyrrhiza glabra elicited by methyl Jasmonate and salicylic acid

Shabani, Leila; Ehsanpour, Ali Akbar; Asghari, Gholamreza; Emami, Jaber

doi:10.1134/s1021443709050069

Cited by 134 publications

(52 citation statements)

References 34 publications

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“…Many reports emphasizes the importance of MJ in eliciting secondary metabolite production in cell and tissue cultures (Kittipongpatana et al 2002;Choi et al 2005;Gandzovska et al 2007;Lystvan et al 2009;Ana Coste et al 2011;Junge Qu et al 2011). MJ treatment has enhanced the accumulation of triterpenoid saponins in cell and tissue cultures of various plants, ginsenoside content in cultured cells (Lu et al 2001) and hairy roots (Yu et al 2002;Ali et al 2006;Kim et al 2009aKim et al , 2009b of P. ginseng, saikosaponin in roots fragments of Bupleurum falcatum (Aoyagi et al 2001;Kim et al 2011), soyasaponin biosynthesis in cultured cells of Glycyrrhiza glabra (Hayashi et al 2003;Lee et al 2004;Shabani et al 2009), asiaticoside production in whole plants (Mangas et al 2006) and hairy roots (Kim et al 2007) of Centella asiatica.…”

Section: Discussionmentioning

confidence: 99%

Elicitor-enhanced production of gymnemic acid in cell suspension cultures of Gymnema sylvestre R. Br.

Veerashree

Anuradha

Kumar

2011

Plant Cell Tiss Organ Cult

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Cell suspension cultures of Gymnema sylvestre treated with four different elicitors, methyl jasmonate (MJ), yeast extract, chitin and pectin were studied for the production of gymnemic acid as gymnemagenin equivalent, that was analyzed by high performance liquid chromatography (HPLC). All the four tested elicitors induced gymnemic acid production in cell suspension cultures. Highest gymnemic acid content was achieved following treatment with yeast extract (100.47 ± 0.28 mg/l), this was followed by MJ (70.43 ± 0.26 mg/l), pectin (64.19 ± 0.23 mg/l) and chitin (62.72 ± 0.13 mg/l). The addition of elicitors has shown a significant influence on cell growth that affected cell growth compared to respective controls. The highest gymnemic acid production was obtained after 20 days of elicitation in cultures treated with 0.5 g l -l yeast extract, it was 5.25-folds greater than in control. These results suggest that the addition of an elicitor to Gymnema sylvestre cell suspension cultures could stimulate and enhance gymnemic acid production. In our present study we could able to overproduce gymnemic acid up to 51.97 ± 0.26 mg l -l (dry weight basis) in yeast extract treated cell suspension cultures.

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Section: Discussionmentioning

confidence: 99%

Elicitor-enhanced production of gymnemic acid in cell suspension cultures of Gymnema sylvestre R. Br.

Veerashree

Anuradha

Kumar

2011

Plant Cell Tiss Organ Cult

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“…The method of elicitation to improve glycyrrhizic acid productiveness was applied in the roots of 65-day old plants of G. glabra. Treatment of plantlets with 0.1-2 mM of methyl jasmonate or with 0.1 and 1 mM salicylic acid enhanced the production of glycyrrhizic acid, as compared to the controls [44]. There are the other ways for increasing the amount of glycyrrhizic acid.…”

mentioning

confidence: 93%

The growth and saponin production of Platycodon grandiflorum (Jacq.) A. DC. (Chinese bellflower) hairy roots cultures maintained in shake flasks and mist bioreactor

et al. 2014

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IntroductionHairy, or transgenic roots are characterized by a rapid and stable growth, which importantly can be initiated and maintained in a hormone-free medium. They often synthetize approximately the same and in some cases even greater amounts of secondary metabolites compared to their mother plants [1,2] and the rate of production remains stable. This makes hairy roots of medicinal plants a good source of valuable compounds and obviously their large-scale culture in bioreactors holds immense potential for the pharmaceutical industry [3].Platycodon grandiflorum (Jacq.) A. DC. (Campanulaceae) is an ornamental plant growing wild in northern Asia, in China, Korea, Japan and east Siberia. For culture it needs light, medium moisture, organically rich, well-drained loams and full sun to part shade.Platycodi radix, the root of P. grandiflorum, has been used as food and in traditional oriental medicine to treat bronchitis, asthma and other pulmonary diseases. Triterpene saponins, called platycosides, are responsible for the medicinal value of this plant material. They have demonstrated a broad spectrum of therapeutic effects, such as antitumor [4][5][6][7], anti-inflammatory [8,9], hepatoprotective [10], antiatherosclerotic [11] and antinociceptive [12]. Additionally, these compounds were found to be a potent adjuvant of specific cellular and humoral immune responses, with the potential use in vaccine production [13][14][15]. Finally, saponins from Platycodi radix stimulated osteoblast differentiation via RUNX2, a principal osteogenic master gene for bone formation [16].Although the extensive pharmacological studies of these valuable compounds were undertaken, to date the sum of saponins, not the chosen, in hairy root cultures of P. grandiflorum was determined only by our research team [17]. Besides, this is the first report of the growth of P. grandiflorum transgenic roots cultured in a mist bioreactor and their yield of saponins. The method of Platycodi radix saponins determination published in "Chinese pharmacopeia" [18] exhibited as no suitable for quantitative analysis of saponins from P. grandiflorum transgenic roots. Therefore, the authors elaborated their own method [17].The compounds mentioned above are the main chemicals found in Platycodi radix. They are typical composed of oleaene backbones with two side chains: one is a 3-O-glucose linked by glycosidic bond, and the other is a 28-O-arabinoserhamnose-xylose-apiose linked by an ester bond [19]. To date, more than 55 triterpenoid saponins have been isolated from Platycodi radix [20]. Examples are platycodins A-I and polygalacins D and D2. According to WHO monographs AbstractThe growth and saponin accumulation were measured in two lines of transgenic hairy roots of Platycodon grandiflorum, Pl 6 and Pl 17, cultured for 8 weeks in 250-ml shake flasks containing 50 ml of hormone-free woody plant medium supplemented with 40 g/l sucrose and in the Pl 17 line cultured for 12 weeks in a 5-l mist bioreactor containing 1.5 l of the same medium. With both methods, the...

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“…These results are in contrast with MeJA effects on other secondary metabolites which often increase upon MeJA treatment [9]. This demonstrated that Maesa saponins are produced in a stable fashion and not affected by MeJA treatment.…”

Section: Saponin Production In In Vitro Culturesmentioning

confidence: 71%

Studies on saponin production in tropical medicinal plants Maesa argentea and Maesa lanceolata

Faizal

Geelen

2015

AIP Conference Proceedings

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Abstract. The continuous need for new compounds with important medicinal activities has lead to the identification and characterization of various plant-derived natural products. As a part of this program, we studied the saponin production from two tropical medicinal plants Maesa argentea and M. lanceolata and evaluated several treatments to enhance their saponin production. In this experiment, we present the analyses of saponin production from greenhouse grown plants by means of TLC and HPLC-MS. We observed that the content of saponin from these plants varied depending on organ and physiological age of the plants. In addition, the impact of elicitors on saponin accumulation on in vitro grown plants was analyzed using TLC. The production of saponin was very stable and not affected by treatment with methyl jasmonate, and salicylic acid. In conclusion, Maesa saponins are constitutively produced in plants and the level of these compounds in plants is mainly affected by the developmental or physiological stage.

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Glycyrrhizin production by in vitro cultured Glycyrrhiza glabra elicited by methyl Jasmonate and salicylic acid

Cited by 134 publications

References 34 publications

Elicitor-enhanced production of gymnemic acid in cell suspension cultures of Gymnema sylvestre R. Br.

Elicitor-enhanced production of gymnemic acid in cell suspension cultures of Gymnema sylvestre R. Br.

The growth and saponin production of Platycodon grandiflorum (Jacq.) A. DC. (Chinese bellflower) hairy roots cultures maintained in shake flasks and mist bioreactor

Studies on saponin production in tropical medicinal plants Maesa argentea and Maesa lanceolata

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