Glycyrrhizin protects human melanocytes from H2O2‑induced oxidative damage via the Nrf2‑dependent induction of HO‑1

Mou, Kuanhou; Pan, Wenjie; Han, Dan; Wen, Xin; Cao, Fang; Miao, Yi; Li, Pan

doi:10.3892/ijmm.2019.4200

Cited by 29 publications

(26 citation statements)

References 42 publications

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“…Bcl-2/Bax ratio has been reported to be an important determinant factor of apoptosis [40]. Ample pieces of evidence have also proven that H 2 O 2 treatment could increase cell apoptosis [32,41]. However, pre-treatment with curcumin inhibited cell apoptosis, which was demonstrated by declined apoptosis rate, reduced protein expression of cleaved-caspase 3 and increased expression level of Bcl-2/Bax ratio.…”

Section: Discussionmentioning

confidence: 99%

Curcumin Protects Human Trophoblast HTR8/SVneo Cells from H2O2-Induced Oxidative Stress by Activating Nrf2 Signaling Pathway

Jiang

Zhang

et al. 2020

Antioxidants

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Pregnancy complications are associated with oxidative stress induced by accumulation of trophoblastic ROS in the placenta. We employed the human trophoblast HTR8/SVneo cell line to determine the effect of curcumin pre-treatment on H2O2-induced oxidative damage in HTR8/Sveo cells. Cells were pretreated with 2.5 or 5 μM curcumin for 24 h, and then incubated with 400 μM H2O2 for another 24 h. The results showed that H2O2 decreased the cell viability and induced excessive accumulation of reactive oxygen species (ROS) in HTR8/Sveo cells. Curcumin pre-treatment effectively protected HTR8/SVneo cells against oxidative stress-induced apoptosis via increasing Bcl-2/Bax ratio and decreasing the protein expression level of cleaved-caspase 3. Moreover, curcumin pre-treatment alleviated the excessive oxidative stress by enhancing the activity of antioxidative enzymes. The antioxidant effect of curcumin was achieved by activating Nrf2 and its downstream antioxidant proteins. In addition, knockdown of Nrf2 by Nrf2-siRNA transfection abolished the protective effects of curcumin on HTR8/SVneo cells against oxidative damage. Taken together, our results show that curcumin could protect HTR8/SVneo cells from H2O2-induced oxidative stress by activating Nrf2 signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Curcumin Protects Human Trophoblast HTR8/SVneo Cells from H2O2-Induced Oxidative Stress by Activating Nrf2 Signaling Pathway

Jiang

Zhang

et al. 2020

Antioxidants

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“…Quercetin also possesses anti‐inflammatory and antioxidative capacity, albeit not studied in vitiligo treatment 96 . Apart from polyphenols, an array of organics, such as glycyrrhizin, afzelin, 6‐Shogaol, simvastatin, aspirin, also demonstrated their antioxidative ability by targeting Nrf2 pathways 97–101 . Vitamin D can ameliorate ROS in melanocytes and restore their viability in oxidative stress through activating Wnt/β‐catenin pathway 102 .…”

Section: Oxidative Stressmentioning

confidence: 99%

Mechanisms of melanocyte death in vitiligo

Chen

2020

Medicinal Research Reviews

161

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Vitiligo is an autoimmune depigment disease results from extensive melanocytes destruction. The destruction of melanocyte is thought to be of multifactorial causation. Genome‐wide associated studies have identified single‐nucleotide polymorphisms in a panel of susceptible loci as risk factors in melanocyte death. But vitiligo onset can't be solely attributed to a susceptive genetic background. Oxidative stress triggered by elevated levels of reactive oxygen species accounts for melanocytic molecular and organelle dysfunction, a minority of melanocyte demise, and melanocyte‐specific antigens exposure. Of note, the self‐responsive immune function directly contributes to the bulk of melanocyte deaths in vitiligo. The aberrantly heightened innate immunity, type‐1‐skewed T helper, and incompetent regulatory T cells tip the balance toward autoreaction and CD8+ cytotoxic T lymphocytes finally execute the killing of melanocytes, possibly alarmed by resident memory T cells. In addition to the well‐established apoptosis and necrosis, we discuss several death modalities like oxeiptosis, ferroptosis, and necroptosis that are probably employed in melanocyte destruction. This review focuses on the various mechanisms of melanocytic death in vitiligo pathogenesis to demonstrate a panorama of that. We hope to provide new insights into vitiligo pathogenesis and treatment strategies by the review.

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“…The name derives from the Greek word 'glykosrhiza' or sweet root. GLR is commonly isolated from Glycyrrhizae Radix in the form of a monoammonium salt (C 42 H 61 O 16 _NH 4 ), but occasionally studies are performed with the magnesium salt of 18β-glycyrrhizic acid (magnesium isoglycyrrhizinate) (Cao et al, 2019;Tan et al, 2018;Zou et al, 2018). Specific methods have been developed to monitor the extraction process and for GLR dosages in biological media (Wang, Shan, & Lü, 2020;Yu et al, 2017;Zeng et al, 2020).…”

Section: Glycyrrhizin (Glr) a Natural Productmentioning

confidence: 99%

Glycyrrhizin: An alternative drug for the treatment of COVID-19 infection and the associated respiratory syndrome?

Bailly

Vergoten

2020

Pharmacology & Therapeutics

252

205

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Safe and efficient drugs to combat the current COVID-19 pandemic are urgently needed. In this context, we have analyzed the anti-coronavirus potential of the natural product glycyrrhizic acid (GLR), a drug used to treat liver diseases (including viral hepatitis) and specific cutaneous inflammation (such as atopic dermatitis) in some countries. The properties of GLR and its primary active metabolite glycyrrhetinic acid are presented and discussed. GLR has shown activities against different viruses, including SARS-associated Human and animal coronaviruses. GLR is a non-hemolytic saponin and a potent immuno-active anti-inflammatory agent which displays both cytoplasmic and membrane effects. At the membrane level, GLR induces cholesterol-dependent disorganization of lipid rafts which are important for the entry of coronavirus into cells. At the intracellular and circulating levels, GLR can trap the high mobility group box 1 protein and thus blocks the alarmin functions of HMGB1. We used molecular docking to characterize further and discuss both the cholesterol-and HMG boxbinding functions of GLR. The membrane and cytoplasmic effects of GLR, coupled with its long-established medical use as a relatively safe drug, make GLR a good candidate to be tested against the SARS-CoV-2 coronavirus, alone and in combination with other drugs. The rational supporting combinations with (hydroxy)chloroquine and tenofovir (two drugs active against SARS-CoV-2) is also discussed. Based on this analysis, we conclude that GLR should be further considered and rapidly evaluated for the treatment of patients with COVID-19.

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Glycyrrhizin protects human melanocytes from H2O2‑induced oxidative damage via the Nrf2‑dependent induction of HO‑1

Cited by 29 publications

References 42 publications

Curcumin Protects Human Trophoblast HTR8/SVneo Cells from H2O2-Induced Oxidative Stress by Activating Nrf2 Signaling Pathway

Curcumin Protects Human Trophoblast HTR8/SVneo Cells from H2O2-Induced Oxidative Stress by Activating Nrf2 Signaling Pathway

Mechanisms of melanocyte death in vitiligo

Glycyrrhizin: An alternative drug for the treatment of COVID-19 infection and the associated respiratory syndrome?

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